gene stock 中文意思是什麼
gene stock
解釋
基因材料-
In order to get the soluble recombinant eo protein and inspect the protein expression status convinently, the egfp and eo gene were ligated into baculovirus transfer vector. with the co - transfecting sf9 cells of baculovirus recombinant transfer vector and linearized viral dna, and plaque purification in the posttransfection procedure, the pure recombinant baculovirus were harvested, which infected the sf9 cells for amplifying to generate a p - l stock. in the meantime, the fluorescence microscopy detection indicated expressed egfp protein to confirm the heterogenous protein expression of recombinant baculovirus. the pi - stock from a pure plaque was used to generate a high liter p - 2 stock, which was determined in liter as 1. 14 107pfu / ml by performing a plaque assay. when a volume of p - 2 stock infected the sf9 cells with moi 5 - 10 for expression, the strong fluorescence was obeserved on the day 3 of postinfection
此外，為了得到可溶性重組eo蛋白並便於觀察重組蛋白的表達情況，我們將egfp基因與eo基因相連插入昆蟲桿狀病毒轉移載體中，與線性桿狀病毒dna共轉染sf9細胞后通過噬斑純化得到純的重組桿狀病毒，將其感染sf9細胞制備p1種子液，同時用熒光顯微鏡觀察綠色熒光蛋白的表達情況剔除表達效果差的重組桿狀病毒。再用p1種子液感染sf9細胞制備高效價的p2種子液。通過病毒液的梯度稀釋和噬斑測定，確定p2種子液的病毒滴度達1 . 14 10 ~ 7pfu ml 。 -
The subject focus on the issues about how to optimize the usage of material for manufacturing, especially on wood plate, the core arithmetic of the system is algorism genetic algorithms using the computer technology, the system is developed to realize the algorithms, which can optimize layout of two - dimensional cutting stock automatically the genetic algorithms overcome shortcomings of some traditional optimization, which can only get the local optimal solutions. the algorithms step out from local optimization to get the global optimization through the gene mutation of chromosome
本課題對目前製造業生產工藝中存在的下料問題，尤其是板式傢具的優化排料問題，進行了深入地分析和研究，將實數型遺傳演算法作為核心演算法，引入到系統設計中，提出了解決板材下料的一套實用演算法，結合計算機編程，實現了二維板材傢具優化下料的自動優化排料系統。 -
The build and construction of ecology include the build and construction of green space overall arrangement, the build and construction of the index, the build and construction of seedling security system, the build and construction of bio - diversity system and the build and construction of the technological economic policy security system etc. these classified build and construction present the logic relation with each other : the build and construct of overall arrangement control the green skeleton of the city ; the index plan ensure the green skeleton can exist forever ; the build and constructi on of seedling security system guarantee the index of city green space can put in practice in the light of plan ; the build and construction of bio - diversity system can afford the abundant gene pool for nursery stock system and can ensure the ecological stability of the city ; and the build of the technological economic policy security system can guarantee the implementation of a serious of build and construction upwards successfully
為了符合生態園林城市的定位，綠地系統需緊緊抓住生態建構和景觀風貌建構兩個方向。其中生態建構包括各類綠地布局建構、指標建構、苗木保障體系建構、生物多樣性體系建構及技術經濟政策保障體系的建構等。這些分類建構互呈邏輯關系：布局建構控制了城市的綠色脈絡，指標規劃保障這個綠色脈絡得以生生不息，苗木保障體系的建構則保證城市綠地指標得以科學實施，生物多樣性體系保障則是為苗木體系提供豐富的基因庫，技術經濟政策保障體系的建立可以保障以上一系列建構的順暢實施。
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