gene targeting 中文意思是什麼

gene targeting 解釋
基因打靶
  • gene : n. 【生物學】基因。 dominant gene顯性基因。
  • targeting : 導向目標
  1. The fusion protein was bactericidal active against staphylococcus aureus. in present study, we will truncate the none channel - forming do main, then attach the agrd to the pore - forming region ( k544 - i626 ) to construct a new engineered multidqmain protein machine - compact engineered peptide targeting staphylococcus aureus. such engineered peptide was constructed by linking the gene of staphylococcal agrd pheromone with the gene of c - terminal ( 1626 ) of colicin la pore - forming region ( k544 - i626 ) with site - directed mutation

    利用點突變方法將金黃色葡萄球菌信息素agrd （ i型， ystcdfim ）的基因引入到大腸菌素fa梭基端1626基因上，並將限制性內切酶sacl酶切位點基因分別引入到大腸菌素fa的p4和k544上，通過酶切、膠回收、連接獲得含大腸菌素ia水性孔道結構域和金黃色葡萄球菌信息素agrd基因的重組質粒。
  2. Here we studied the relationship of various factors and the quality of protoplasts. which maybe could be the basic of moss gene targeting. results showed : inoculated the spores onto diferrent kinds of media, such as ms, benecke and knop, we found that there was no difference when the spores germinated and differentiated into cauliform soon

    通過對立碗蘚的無菌培養和原生質體操作發現： （ 1 ）立碗蘚孢朔接種在無菌ms 、 benecke 、 knop培養基上，均可萌發產生原絲體，但不久便分化為莖葉體，很難長期保持其原絲體狀態，不同培養基條件下原絲體狀態有所不同。
  3. 4. engineering dhqase ( arod ) - deficient e. coli mutant with a second copy of the arob gene gene targeting technique was used to disrupt the arod gene in e. coli chromosome. the mutant 31bk was engineered, in which homologous recombination of the arobkanr gene cassette into the arod locus ( arod : : arobkanr ) of the e. coli strain atcc31884 genome utilized the helper plasmid pkd46 with red system. the host cell 31bk lacked catalytic activity of dhqase ( arod ) and had a second copy of the arob gene, so it improved carbon flow into the quinic acid biosynthesis direction

    構建宿主菌基因精確定位突變株31bk （ arod : : arobkan ~ r ）為了改變代謝途徑脫氫奎尼酸（ dhq ）分支點上的代謝流量，使之充分流向目的產物奎尼酸合成方向，利用基因打靶技術構建了31884宿主菌arod基因精確定位插入突變體，使dhq脫水酶（ dhqase ）失活，阻斷了碳代謝流流向芳香氨基酸生成的方向，同時用同源重組的方法將arob基因定位整合入染色體上，解除了限速酶對碳代謝流通過共同途徑到達dhq的阻遏影響，並減輕代謝負擔。
  4. Effect of sirna targeting leptin gene on biological behaviors of rat hepatic stellate cells

    對大鼠肝星狀細胞生物學特性的影響
  5. This sequence emergences fourteen times from 1000 ests library indicts that it is a middle affluently gene in cdna library. the cdna of 634 basepairs contains an open reading frame of 339 nucleotides encoding a novel nonspecific lipid transfer protein. the first 23 amino acids constitute the putative signal peptide, characteristic for targeting to the secretory pathway

    測得th - nsltp序列全長為634bp ，含有一個非特異性脂轉移蛋白與植物耐逆性的相關性研究編碼112個氨基酸的閱讀框架， n端的23個氨基酸組成一段信號肽序列，表明它可能和分泌有關。
  6. The moss physcomitrella patens ( funariales bryophyta ) is being developed as a model system for plant biological studies. its gene targeting efficiency can be compared with that observed in saccharomyces cerevisiae

    小立碗蘚是最近被開發出來的用於研究基因打靶的模式植物，其基因打靶的效率可以與酵母相比。
  7. The development of gene targeting and animal cloning thechnology makes the site - specific integration of foreign gene become possible

    隨著基因打靶技術和動物克隆技術技術的發展，外源基因在高等哺乳動物基因組中的定點整合成為了可能。
  8. I have used low copy pbin19 and single copy pmw755i5j binary vectors as backbone plasmids, to create a gene targeting insertion vector designated gfp tnos. after agro - infiltration into transgenic nicotiana benthamiana 16c, progeny were analyzed genetically for phenotypic changes, sirna accumulation, and dna methylation

    採用農桿菌浸潤法（ agro - infiltration ）感染轉基因本生煙16c ，並對同源基因瞬時表達所引起的植物表型變化、小分子rna的產生、 dna甲基化程度、以及相關性狀在後代中的遺傳情況進行了檢查。
  9. Researchers at the university of pennsylvania ' s school of medicine have found by targeting the function of a single gene that it is possible to inhibit bone decay while simultaneously stimulating bone formation

    濱西凡尼亞州大學醫學院研究員發現通過單個基因的靶向作用可抑制骨衰退而同時刺激骨形成。
  10. According to the gene sequence and secondary structure of hcv ns5b, we design the sirnas targeting ns5b gene following with the requirement for sirnas design from tuschl et. al and synthesize it from dharmacon company ; hepg2 cell stably expressing ns5b - egfp protein was trasfected by synthesized sirnas with electroportion, the non - transfected cell and non - specific sirnas transfected cell are c onsidered as control group ; inhibitory effect of sirnas was investigated by fluorescence microscope with dapi dyeing and by semi - quantitative rt - pcr

    然後根據dsrna設計原則，結合nssb基因的序列特徵，藉助生物信息學軟體設計了針對nssb基因的sirnas ，並交由公司化學合成；電穿孔法轉染上述穩定轉染的細胞克隆，同時分別以非特異的sirnas轉染組和空白轉染組為對照， dapi染色后通過熒光顯微鏡和內標化rtpcr檢測，初步證實了化學合成的sirnas可以特異阻斷nssb基因的表達。
  11. Research of multiple locus gene targeting in vivo of siniperca

    多位點基因打靶技術的鱖魚體內實驗研究
  12. Advance in research on targeting of gene therapy

    腫瘤基因治療的靶向性研究進展
  13. Then some necessary elements such as gfp gene, neo gene were inserted between the loxp site and the loxs 11 site and obtained a new gene targeting vector pioxgfp. on the other hand, the chicken v - ifn cdna was cloned into the vector plox between the loxp site and the lox511 site and got another gene targeting vector ploxifn

    合成loxp和lox511位點，將其同方向地克隆到載體pegfp - 1的多克隆位點之內，構建成含有lox位點的通用載體? ? plox ，然後將報告基因gfp及neo基因片段克隆到載體plox上的loxp和lox511序列之間，構建成一基因打靶載體? ploxgfp 。
  14. The gene targeting vector was planning to be constructed by using thrombopoietin gene ( tpo ) gene as purpose gene, as 1 - casein gene as targeting gene, the 5 " sequence of - casein gene as promotor

    以綿羊-酪蛋白基因的5調控區為啟動子，人血小板生成素基因為目的基因，奶牛s1 -酪蛋白基因為靶向基因。
  15. At present, there are several approaches to targeted gene disruption ( or gene targeting, as it ' s usually called ) that can be customised to attack anywhere in the genome, but they ' re all highly error - prone, disrupting other locations a great deal

    目前，有幾種方法可用於打靶的基因破壞（或稱基因打靶，實際名稱也是這樣） ，它可以定製，用來攻擊基因組的任何地方，但它們全都容易出錯，在非常多的地方破壞其他位點。
  16. But gene targeting was mainly applied to such field as bacterium, yeast and some epiphyte at present, seldom used in higher plants because of the lower frequency of homologous recombination

    但基因打靶技術目前主要應用於細菌，酵母和一些絲狀真菌，在高等植物中應用極少。原因是高等真核生物中同源重組的發生頻率極低。
  17. Cloning of the genomic fragments of bovine 1, 3 - galactosyltransferase gene and construction of a promotorless targeting vector

    半乳糖轉移酶基因部分片段的克隆及無啟動子打靶載體的構建
  18. Thus, the present study was undertaken to explore the possibility of constructing thrombopoietin ( tpo ) gene targeting vector specially expressed in mammary gland

    為此，本研究對人血小板生成素乳腺表達打靶載體的構建進行了研究。
  19. It led to a new technology - - gene targeting

    他導致了新科學的誕生基因目標。
  20. It led to a new technology, gene targeting

    這也引出一項新的技術? ?基因靶向技術。
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