gene walking 中文意思是什麼

gene walking 解釋
基因巡查法
  • gene : n. 【生物學】基因。 dominant gene顯性基因。
  • walking : n. 1. 步行;步法,步態。2. 道路狀態。adj. 1. 步行(用)的。2. (能)行走的。3. 解僱的,被免職的。
  1. The c - phycocyanin ( cpc ) operon of blue - green alga ( or cyanobacteria ) arthrospira platensis fachb341 was cloned, sequenced and characterized by using chromoseme walking method. the sequence includes cpcb gene ( 519 bp ), cpca gene ( 489 bp ), cpch gene ( 357 bp ), and upstream sequence of cpcb ( 427 bp ) and upstream sequence of cpch genes ( 184 bp ), 111 bp of phycocyanin intergenetic spacer ( pc - igs ). upstream sequence of cpcb gene was ligated into promoter - probe vector pegfp - 1 and transformed into three systems : e. coli, synechocystis pcc 6803 and a. platensis fachb341 by supersonic and electrophoresis methods

    根據genbank中報道的節旋藻藻藍蛋白基因序列設計引物,首先克隆了鈍頂節旋藻( arthrospiraplatensisfachb341 )藻藍蛋白操縱子中亞基基因、亞基基因部分序列及二者之間的間隔區序列( pc - igs )並進行序列測定,然後根據此測序結果設計引物,通過染色體步移法克隆得到藻藍蛋白操縱子長度為2086bp的基因片段,其中包括藻藍蛋白亞基基因( cpcb , 519bp ) ,亞基基因( cpca , 489bp ) ,連接蛋白h基因( cpch , 357bp ) ,亞基基因上游啟動子序列( 427bp )以及各基因之間的間隔區( pc - igs , 111bp ; cpch與cpca間隔區, 184bp ) 。
  2. Using random primed gene walking pcr ( rpgw - pcr ), ctsox2 gene was cloned and sequenced from the digested genomic dna of trionyx sinensis. compared with the mouse and chicken sox2, ctsox2 shared 86 % and 91 % nucleotide homology respectively, and 93 % and 97 % amino acid identity respectively

    天i消化過的中華鱉( trio矛移優sinensis )基因組dna中克隆了c眨治xz基因( 72obp ) ,它與鼠、鳥的及戲2基因分別有85 %和91 %的核昔酸同源性, 93 %和97 %的氨基酸同源性。
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