gene sequence 中文意思是什麼

gene sequence 解釋
基因序列
  • gene : n. 【生物學】基因。 dominant gene顯性基因。
  • sequence : n 1 繼續；接續；連續。2 順序；程序；次第；關系；關聯。3 後果；結果；接著發生的事；後事；後文。4 ...
  1. Recently, we know the number of muntjac deer chromosomes varies from 6 to 48, and there are karyotypic polymorphism intra - species relationships. these animals are extremely useful for phylogenetic studies. in order to discuss the phylogenetic relationships of muntiacinae, we have studied the nuclear gene sequence of muntiacus muntjak, muntiacus crinifrons, muntiacus reevesi, and elaphodus cephalophus

    為了探討麂亞科動物間的親緣關系，本論文以赤麂（ muntiacusmuntjak ） 、黑麂（ muntiacuscrinifrons ） 、小麂（ muntiacusreevesi ）和毛冠鹿（ elaphoduscephalophus ）這4種麂亞科動物為材料，以核基因序列為研究對象，作進一步的研究。
  2. The result showed that the homology rate of pila gene among the 5 avian pathogenic e. coli strains tested and one human e. coli were from 89. 8 % to 91. 1 %, and the homology rate of amino acid were from 88. 5 % to 91. 8 %. the homology rate of pila gene sequence among 5 avian pathogenic e. coli strains tested and avian pathogenic e. coli reported ( serotype o1, o2, o78 ) were from 87. 8 % to 90. 2 %, and the homology rate of amino acid were from 84. 6 % to 91. 2 %. there had homology in avian pathogenic e. coli. there had some common antigen side in type 1 pili of avian pathogenic e. coli

    結果表明：運用msha法檢測1型菌毛的檢出率為80 （ 36 45 ） ， pcr法的檢出率為95 . 5 （ 43 45 ） ， pcr方法用於1型菌毛的檢測比msha更加敏感、快速、特異性強；選擇5株優勢血清型雞源致病性大腸桿菌代表株（ o _ （ 89 ） ， o _ （ 119 ） ， o _ （ 141 ） ， o _ （ 127 ） ）的1型菌毛pila基因的pcr擴增片段經純化后，分別定向克隆到puc18質粒的多克隆位點，構建了含有目的基因片段的克隆質粒，並轉化到dh5株大腸桿菌載體菌中，篩選獲得陽性克隆菌株。
  3. Deduced amino acid sequence of s1, s2, pvin were also highly homologous each other ( 98 %, 99 % in each case ). the stilbene synthase genes were excised from the plasmids by bamh i and sac i digestion and intergrated into a binary vector, pbi121 and pev2, from which the p - glucuronidase ( gus ) gene sequence had been removed by the same digestion to prepare a 35s promoter - stilbene synthase 2 - nopaline synthase polyadenylation site construct and a tfp2 promoter - stilbene synthase 1 - nopaline synthase polyadenylation site construct. the recombinant plasmids were called pbs2, pev2s 1. respectively

    用bamhi和saci同時酶切ps2 （ s2表示來自雷司令的芪合酶基因） 、 ps1 （ s1表示來自粉紅玫瑰的芪合酶基因）以及pbi121 、 pev2 ，使得s2 、 s1分別插入替代pbi121 、 pev2中的gus基因，構建成植物表達載體pbs2 、 pev2s1 ， pbs2中含camv35s組成型啟動子，使s2基因能在番茄植株的各個部位表達； pev2s1則含有果實特異性啟動子tfp2 ，使s1基因只在番茄果實中表達。
  4. Gene sequence analysis showed that the fragment we have obtained has 94 % and 90 % homology with common buckwheat storage protein and legumins, respectively. meanwhile, by analyzing the amino acid sequence, it shares 93 % - 83 % homology with legumins from common buckwheat and sword bean

    採用上述方法獲得的tb22kda核苷酸序列與甜蕎過敏性貯藏蛋白及豆球類蛋白的同源性分別達到94和90 ；氨基酸序列與來自甜蕎中的球蛋白，刀豆蛋白有93 - 83同源性。
  5. This is the first report worldwide of the allergenic protein gene sequence from tartary buckwheat

    來源於苦蕎中的過敏蛋白基因序列在國內外尚屬首次報道。
  6. The virus was purified by ultracentrifuge. according to the ns gene sequence published by genbank, one primer t - 1 was designed to amplify the cdna by reverse transcript. the other primers nsl - u / ns1 - 1 and ns2 - u / ns2 - 1. hns2 - u were designed to amplify the ns1, ns2 and hns2 gene

    本實驗用接種雞胚的病毒增殖方法獲得了a / chicken / mudanjiang / 0823 / 00 （ h9n2 ）分離株的大量增殖，增殖病毒經差速離心進行純化，經超迷離心濃縮。
  7. We designed one primer pairs ctb - 1, ctb - 2 to amplify about 580bp of cyt b gene sequence as a molecular marker to analyze phylogenetic relationship of 14 species of oedipodidae in china. dna sequences were aligned using clustal x, followed by refinement by eye based on the corresponding deduced amino acid sequences. after cutting off 5 " and 3 " termini unaligned sequences, we get 462 bp segment

    本研究從分子生物學角度入手，採用cytb作為分子標記，採用自行設計的一對cytb基因特異引物ctb - 1 、 ctb - 2 ，通過pcr技術，共獲得斑翅蝗科4個亞科14個種的代表個體以及癩蝗科1個種的代表個體的580bp左右的cytb部分序列。
  8. Analysis of mtdna cyt b gene sequence polymorphism in qinchuan cattle

    基因序列多態性分析
  9. Reconstruction of evolutionary tree based on information about short - range correlation of nucleotides in gene sequence

    以核苷酸短程關聯為基礎的進化樹重建
  10. Glp - 1 is based on the proglucagon gene sequence. it is secreted mainly as " truncated " glp - l ( 7 - 36 ) amide with more activities

    Glp - 1盡管和胰高血糖素有48的同源性，它們的作用卻不同。
  11. Three chloroplast transformation vectors including pds16s - cat, ptn1269 - bar and psp72 - n5 - bar - n3 were constructed, using ! 6s rrna or chln gene sequence as a homologous segment and cat or bar as a selective marker gene, respectively. foreign genes were introduced to the cells of d. salina by microprojectile bombardment method and a pilot chloroplast tran

    3 .杜氏鹽藻葉綠體165出na基因的克隆和轉化載體的構建根據杜氏鹽藻的近緣藻類的葉綠體基因組序列資料，克隆了杜氏鹽藻葉綠體16srrna基因部分序列1100bp ，並利用克隆的16srrna鄭州大學2003年博士學位論文
  12. When you look at the gene sequencing that the university has done, the experience with influenza virus is that for efficient human to human spread which will then give rise to pandemic, it usually incorporates human influenza gene viruses and the analysis of gene sequence of the two cases, particularly of the brother, did not show the sequence

    雞只關在街市裡面一、兩個星期、四個星期，現時每天有數十萬雞只在街市，難免有一、兩只會感染禽流感，若這些雞只無法賣出，就有機會把病菌傳播給其他雞只。
  13. Patent law has formed the mechanism that balances the different interest of the society, such as the patent right ca n ' t be abused etc. in fact, gene sequence patent extend industrial circles " monopoly right to bring them more interest

    就一國內部來講，傳統專利法在調整利益關繫上的實踐中已形成了一種衡平機制，如對基礎理論不能壟斷、獲得的專利壟斷權不能超出其做出的貢獻、不能濫用專利權、對技術的進一步的開發、研究不能壟斷等。
  14. According to the gene sequence and secondary structure of hcv ns5b, we design the sirnas targeting ns5b gene following with the requirement for sirnas design from tuschl et. al and synthesize it from dharmacon company ; hepg2 cell stably expressing ns5b - egfp protein was trasfected by synthesized sirnas with electroportion, the non - transfected cell and non - specific sirnas transfected cell are c onsidered as control group ; inhibitory effect of sirnas was investigated by fluorescence microscope with dapi dyeing and by semi - quantitative rt - pcr

    然後根據dsrna設計原則，結合nssb基因的序列特徵，藉助生物信息學軟體設計了針對nssb基因的sirnas ，並交由公司化學合成；電穿孔法轉染上述穩定轉染的細胞克隆，同時分別以非特異的sirnas轉染組和空白轉染組為對照， dapi染色后通過熒光顯微鏡和內標化rtpcr檢測，初步證實了化學合成的sirnas可以特異阻斷nssb基因的表達。
  15. A pair of primers were designed and synthesized based on the published ge gene sequence of prv - rice strain for amplifying ge gene of prv min - a, yielding a 1. 7kb band. the segment was linked to puc19 plasma dna by means of t4 dna ligase, transformed into e. coli jm109 permissive cells, and incubated on lb fray containg amp, x - gal and iptg. small amount of plasma was extracted by base cleavaging for enzyme digest analysis and pcr, resulting in recombinant plasma puge dna containing prv ge

    用t _ 4dna連接酶使ge基因與經bamhi 、 kpni同樣雙酶切的puc19質粒dna連接；用連接產物轉化大腸桿菌jml09感受態細胞，置含amp 、 x - gal和iptg的lb平板上培養12 20小時；挑取白色菌落於選擇性培養基擴大培養，堿裂解法小量提取質粒dna ，並進行酶切分析鑒定，結果獲得整合有prvge基因的重組質粒pugedna ，並與其它prv分離株進行ge基因序列同源性分析。
  16. Compared with a reported cmv - cp gene sequence, the homology of nucleotide sequences were 100 %. the sequencing result also demonstrated the recombinant vector pet - 22b - cp has a proper orf encoding 218 amino acids. the recombinant vector was transformed into bl21 ( de3 ) cells. transformants were grown and induced by the addition of isothiopropylgalactoside ( iptg ) to a concentration of imm with continued shaking at 37 ?

    酶切鑒定及序列測定表明，重組表達質粒pet - 22b - cp連接區域符合設計要求，具有正確的開放閱讀框架，插入片段含有218個氨基酸的完整編碼區，其核苷酸序列與報道的cmv - cp基因的同源性為100 。
  17. The mrna and protein expression were assayed by rt - pcr and sds - page. the results were found that the specific 740bp dna bases of il - 6 was detected by rt - pcr in the recombinant bacteria and a new protein band was found in sds - page with molecular mass of about 49 kda which is consisted of a 23 kda protein deduced from the il - 6 gene sequence and gst ( 26 kda )

    以iptg誘導融合蛋白表達，經rt - pcr檢測發現740bp的特異性il - 6條帶；通過sds - page分析，在49kd處出現gst ? pil6融合蛋白條帶，其表達量占總細菌蛋白量的30 ，證明豬il - 6基因得到了正確轉錄和表達。
  18. According to published chil - 15 gene sequence, a pair of primers were designed and synthesized to clone chil - 15 cdna from cona - activated chicken splenocytes by rt - pcr. recombinant plasmid puc19chil - 15 carrying the chil - 15 gene was constructed

    雞il - 15 （ chil - 15 ）是一種新發現的且與il - 2活性相似的細胞因子，作為疫苗佐劑研究的重要候選細胞因子有巨大潛力。
  19. Part two : studies ; l. the sox gene of dinodon refozonatum was amplified by using a pair of primers which can amplify the conservative motif ( hmg - box ) of human sry gene. the amplification band was observed in both male and female dinodon refozonatum, whose length was consistent with that of human sry gene, which about 220bp. the result of sscp analysis showed that there were many differences in the sox gene sequence between dinodon refozonatum and human, and there was a few differences between male and female dinodon refozonatum. 2. using a pair of degenerate primers based on the conservative region, hmg - box, of human sry gene, six different fragments were amplified from either female or male dinodon refozonatum, then cloned by using pmd18 - t vector and sequenced

    結果顯示： （ 1 ）赤鏈蛇基因的擴增片段與人sry基因擴增片斷大小相同，為220bp左右； （ 2 ）雌、雄赤鏈蛇sox基因的擴增片段大小雖然與人的相同，但其單鏈遷移率與人的有較大差異，而且雌雄個體間有明顯差異，預示該基因的dna序列在雌雄個體中可能有差異； 2 、參照人sry基因hmg - box保守區的序列，又設計一對兼并引物，擴增了赤鏈蛇的sox基因，並對擴增產物進行了克隆和測序。
  20. Now, the structure gene sequence and 3 ' - end gene sequence have been logged on genbank. the accession number is ay044918

    目前，此過敏蛋白tb22kda的結構基因與3 』端基因序列已在genbank登錄，登錄號為ay044918 。
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