gene-enzyme 中文意思是什麼

Two primers, designed according to the conserved regions of ban gene in arabidopsis thaliana, were used to amplify the ban homologous fragments from the genomic dna of brassica napus, b. chinensisl, b. juncea, a. thaliana and other cultural plants of cruciferae. the very similar pcr fragments were obtained from all the amplifications, which implicated that ban may be a conserved gene existing widely in the genomes of cruciferae. pcr fragments were cloned and confirmed by restriction enzyme digestion

參照擬南芥（ arabidopsisthaliana ） ban基因與cdna設計引物，對甘藍型、白菜型、芥菜型油菜，擬南芥及其它十字花科栽培品種的基因組dna進行pcr擴增，均擴增出與擬南芥ban基因擴增片段大小極其相似的dna片段，提示ban可能廣泛存在於十字花科植物中。

A double - strand cdna fragment of dh ( diapause hormone ) gene was obtained from kt - pcr amplification. the fragment was digested by two restriction enzyme nde 1 / ecor 1 and then was joined with pet - 30a ( + ) plasmid which was digested by nde 1 / ecor 1 too. then the outcome was transformed into escherichia coli bl21

Pcr產物經nde / ecor雙酶切后，與同樣雙酶切的pet - 30a （ + ）質粒連接並轉化至大腸桿菌（ escherichiacoli ） bl21中，經檢測篩選，成功得到陽性克隆。

Enhance the expression of b. subtillis fibrinolysis enzyme by degq gene

豆豉纖溶酶的研究進展

Relationships between angiotensin - converting enzyme gene polymorphism and lacunar infarction in early stage of essential hypertension

血管緊張素轉換酶基因多態性與高血壓病早期腔隙性腦梗死的關系

Sub - - clone of s, . / hbsag fusion gene : pbuescripts, . / hbsag and ppiczaa were digested separately by xhoi and xbai enzyme, and were linked under t4 dna ligase, ppiczaa s, / hbsag was constructed and transformed to e. coli

Hbsag質粒與ppiczaa載體分別經xhol和xbaln切，再在t4dna連接酶作用下進行連接，獲得工程菌表達型ppiczaas ； hbsag質粒，轉化大腸桿菌t0p10細胞，經xhol和xbal與sacll和xbal酶切電泳，證實s ； 。

Now transgenic cell lines that express the protein encoded by ugt gene were broadly used instead of liver microsomes. the enzyme expressed in cell lines helps to realize the function of a specific gene and will provide direct information related to the isozyme interested

『為解決上述問題，國際上人們己經廣泛採用建立各種ugt轉基因細胞系的方式試驗，在明確基因構成的倩況下直接獲得由特定基因所表達的ugt同工酶， fi什對性地研究該酶對藥物的代謝作用。

Quinic acid, used shikimate pathway in e. coli, it is necessary to extend metabolic pathway by introduction of a heterogenous gene qutb into the host cell. double specific enzyme genes arog, qutb or three ones arog, qutb, arob were co - expressed in a single plasmid pbv220 to improve the enzymes " rate - limiting reactions. modifications of e. coli chromosome by both disruption of the arod gene and directed - site insertion of the arob gene resulted in the change of carbon flow redirected into the quinic acid biosynthesis branch

利用大腸桿菌莽草酸途徑合成新的代謝物奎尼酸，須在宿主細胞引入異源酶基因擴展代謝途徑；串聯表達酶基因，同時適量增加不同種屬的多個關鍵酶酶量，改善限速反應；利用同源重組進行基因整合和基因破壞，改造染色體結構定向改變微生物代謝途徑；目的是將碳代謝流最大程度的引向奎尼酸生成的方向。

Pcr and enzyme acitivity check on chitin agar showed that the chitinase gene fragment existed and expressed in the wildtype strains. antagonistic activity test in vitro suggest that the transformants remained the ability to produce antibiotics. the recombinant strains showed an increased biocontrol efficacy against wheat take - all and rhizoctonia sheath blight in greenhouse

對小麥全蝕病和紋枯病的盆栽試驗結果表明工程菌株p25113一9的防病作用強於野生菌株，尤其是對小麥全蝕病的防治作用，其防治效果同野生菌株ap113和ap25相比，分別增長了22

Many legume plants may produce the beneficial isoflavones, which are vital to the formation of nitrogen - fixing root - nodules and the disease resistance response of plants. white clover ( trifolium repens ), a high quality legume forage, however, has little isoflavones. to improve the isoflavone contents of such forage, an available strategy is to introduce gene ( s ) encoding the key enzyme ( s ) in the biosynthesis pathway of insoflavones

許多豆科植物中都含有異黃酮類化合物，異黃酮對植物根瘤的形成和植物的防禦反應至關重要，但是在牧草白三葉中缺乏這種有益的化合物，因此通過轉基因實驗將異黃酮合成酶基因導入白三葉中，提高其異黃酮的含量，增加白三葉的營養價值。

The other amylase activity is 40 times of native amylase. the non - amylase activity gene has 9 base changes, resulting in 5 amino acid changes from the native enzyme

其中，無酶功能基因突變位點最多，導致全長編碼區內5個氨基酸突變，低酶活和高酶活基因各兩個突變位點。

It was suggested that the gene t13m11. 8 could be the ast candidate gene. this gene was 1432bp long with 6 exons and 5 introns. the putative protein of t13m11. 8 gene was highly homologous to dihydroflavonol 4 - reductase ( dfr ), which was an important enzyme in the anthocyanin biosynthesis pathway

該基因的dna序列長1432bp ，含有6個外顯子和5個內含子，編碼的蛋白與花青苷生物合成途徑中的二氫黃酮醇4 -還原酶有較高的同源性。

To target this mitochondrial enzyme into chloroplast, the cdna sequence of mnsod was fused to a chloroplast transit peptide from a pea rubisco small subunit gene, whereas expression of the chimeric gene was driven by the camv 35s promoter

Pchlsod質粒含有煙草mnsod基因的cdna序列，與豌豆核糖體小亞基葉綠體引物肽（ tp ）的編碼基因序列構成融合基因，由35s啟動子調控。 npt基因為選擇標記基因， pgv2260為輔助質粒。

They contained an antisense constructed for the spruce gene encoding ccr ( cinnamoyl alcohol dehydrogenase ), an enzyme of monolignol synthesis. the antisense rna method is a technique for reducing the expression of a resident target gene. the transgenic sublines were produced by particle bombardment at the dept of forest genetics, swedish university of agricultural sciences

本項目研究的目的就是採用瑞典農業大學構建的反義ccr基因轉導的挪威雲杉細胞愈傷組織，通過誘導產生再生植株，並檢測證實該基因是否表達及其它相關基因的表達狀況，為培育出低木質素的轉基因挪威雲杉新品種奠定物質和理論基礎。

The recombinants were constructed by transforming ppic9 a - xynb into p. pastoris gs115. the assay results revealed that the xylanase gene xynb was overexpressed and secreted effectually in p. pastoris. in 3l fermentor the expression level of xylanase xynba exceeded 1200iu / ml and the expressed xylanase had normal bioactivity. the molecule weight of xynba was determined as about 31kd which is higher than 23kd of original enzyme xynb from streptomyces olivaceoviridis a1. xynbb was gotten by deglycasylation of xynba, whose molecule weight returned to 23kd. we comparised the enzymatic properties of xynba expressed in p. pastoris, xynbb deglycasylated from xynba and xynb produced from streptomyces olivaceoviridis al : there was little difference among the three enzymes on optimal ph, the optimal ph of xynb and xynba were both 5. 2, the optimal ph of xynbb was 5. 0 ; the optimal temperature of xynb and xynba were both 60 c, while the optimal temperature of xynbb was 50 ? ; because of glycosylation the thermal stability of xynba was better than xynb and xynbb ; the specific activity of xynba and xynbb were 883. 88iu / mg and 832. 5hu / mg respectively, which were both lower than 2814. 45iu / mg of xynb ; the km values of xynb and xynba were similar to each other which were 21. 56 ( g / kg ) and 20. 87 ( g / kg ), while the km value of xynbb was 27. 10 ( g / kg ) ; the fmax of xynba and xynbb were 4568umol / mg. min and 5329umol / mg. min respectively which were lower than 27623 umol / mg. min of xynb ; additionally all of the three enzymes did not display cellulase activity. they all had well resistance to pepsion and trypsin, and were not sensitive to metal iron, surface active agent and chelating agent. the analysis of different xylans enzymatic hydrolysate revealed : by xynba, that the main constitutions of enzymatic hydrolysate of birch wood xylans were xylotriose and xyloquaiose, which account for 68. 43 % and 16. 50 % respectively, additionally there was 11. 79 % of xylobiose ; the main constitutions of enzymatic hydrolysate of corncobs xylans were xylobiose and xylotriose, which account for 81. 78 % and 11. 55 %. the result indicated that this xylanase was a kind of 1, 4 - b - d - xylanohydrolase and was fit to used in industrial procession of xylooligosacc harides

進一步對xynba進行了脫糖基化處理得到xynbb ，其分子量恢復到23kd ，證明xynba是糖基化蛋白。通過對畢赤酵母重組表達的木聚糖酶xynba 、脫糖基化的木聚糖酶xynbb以及橄欖綠鏈黴菌a1所產原酶xynb之間酶學性質的比較發現：三種酶的最適ph差異不大， xynb和xynba均為5 . 2 ， xynbb為5 . 0 ； xynb和xynba的最適溫度均為60 ， xynbb降為50 ：在耐熱性上， xynba由於糖基化作用熱穩定性明顯高於未糖基化的xynb和xynbb ； xynba和xynbb的比活性分別為883 . 88iu mg和832 . 51iu mg ，明顯低於原酶的比活2814 . 45iu mg ； xynb和xynba的km值相當，分別為21 . 56 （ g kg ）和20 . 87 （ g kg ） ，而xynbb的km值較大為27 . 10 （ g kg ） ； xynba和xynbb的vmax相差不大，分別為4568 mol mg ? min和5329 mol mg ? min ，明顯低於xynb的27623 mol mg ? min此外三種酶均無纖維素酶活性，對胃蛋白酶和胰蛋白酶有很好的抗性，且對作用環境中的各種離子、表面活性劑、螯合劑不敏感。通過對不同木聚糖的酶解產物的糖份分析發現：以樺木木聚糖為底物時，酶解產物主要為木三糖和木四糖，含量分別為68 . 43和16 . 50 ，另外還含有11 . 79的木二糖；以玉米芯木聚糖為底物時，酶解產物主要為木二糖和木三糖，含量分別為81 . 78和11 . 55 。

Rinat b, hubert c, corvol p, et al. pcr detection of the insertion / deletion polymorphism of the human angiotensin converting enzyme gene j. nucl acids res, 1990, 20 : 1433

和姬苓，王永福，楊國安，等.血管緊張素受體? 1基因多態性與腦血管病的關系j .臨床神經病學雜志， 2007 ， 20 : 12

In thl1 gene, there are some famililar enzyme digest sites including bamh i, hind, sac i and sal i. the sequence of thl1l from brassica oleracea l. shows 99 % identity to the sequence of thll from brassica napus l. there are some phosphorylation sites and an active site of thoiredoxin h members consisting of cppc in thll protein

甘藍和油菜thl1基因序列有5個堿基的差異，而氨基酸僅有2個氨基酸的變異，同源性達99 。在thl1蛋白的氨基酸序列中發現了一個硫氧還蛋白家族的活性位點（ cppc ）和多個磷酸化位點。

Secondly, analysis of peroxidase isoenzyme with polyacrylamidedel electrophoresis for was performed in order to investigate the changes of gene expression under sound stimulation. it could be seen from electrophoresis gel that each group had 6 enzyme bands. new enzyme band in pod electrophoretogram was n ' t detected for stressed groups

此外，在部分實驗組的培養基中加入不同濃度的蛋白質合成抑制劑環己亞胺酮（ chm ）后發現， pod和cat的活性有所降低，暗示著聲波處理使保護酶活性升高的原因可能是聲波處理促進了細胞內酶的合成。

Plants can obtain high doses glyphosate tolerance if the enzyme of this gene is over produced and accumulated or herbicide - insensitive enzymes produced due to some amino acid mutation in active sites

植物中epsp合成酶的過量表達或某些活性位點氨基酸的突變對高劑量的草甘膦有較強的耐受性。

Cloning of the glyphosate n - acetyltransferase gene from soil total dna and its characterization of enzyme activity

乙酰轉移酶基因的克隆及酶學特性分析

Abstract : the polymorphism of angiotensinogen gene at position 174 was studied in 90 cases of essential hypertension patients and 109 controls by pcr, restriction enzyme analysis and electrophoresis methods. the results showed the distribution of genetypes in hypertension group was significantly different from that of controls group. this suggested there is a correlation between the variant of agt174 and hypertension

摘要本文採用pcr 、限制性酶切和電泳分型等方法，分別對90例原發性高血壓患者和109例正常人血管緊張素原基因多態位點agt174進行了檢測，結果表明，高血壓組中三種基因型的分佈與對照組顯著不同，提高該位點變異與原發性高血壓的發生相關。