hela cells 中文意思是什麼
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The effectiveness of the nuclear localization signal selected was clearly tested in initial experiments. based on this, the co - transfection systems used in the study was established. we first observed the multinucleate cells and chromatin bridges in cultured hela cells when the cells are marked with gfp and hochest33342, after co - transfection with the gfp expression plasmids and rnai plasmids rhe or rhc
當分別共轉染附加kozac序列和核定位信號的gfp與人集縮素smc亞基hcap一e特異的rnai質粒rhe和人集縮素smc亞基hca屍一c的f { nai質粒rhc時，都觀察到gfp和hochest33342標記的轉染后hela細胞表現多核和染色質橋現象。 -
The influence of solanum lyratum thunb extract on apoptosis and the expression of fas fasl genes in hela cells
川芎嗪對成體大鼠局灶性腦缺血后皮質和紋狀體半暗帶細胞增殖的作用 -
Experimental study of trichosanthin ' s effect on hela cells proliferation
細胞增殖的實驗研究 -
The results from rt - pcr indicated the knockdown of mrna level of hcap - e and hcap - c in the co - transfeted hela cells
進行rt一pcr檢測表明轉染后細胞內hcap一e和hcap一c在袱na水平被下調。 -
Methods : 1 ) 12h after irradiation, the cell cycle of nih3t3 cells was determined by flow of cytometry and the ratio of alternations in p16 gene exon - 2 was evaluated through pcr - sscp. 2 ) the content of mda, the activities of the sod and gsh - px in the supernatant of nih3t3 cells and the cells were measured by detecting kits immediately after irradiation. 3 ) the level of matrix metalloproteinase - 2 ( mmp - 2 ) in hela cells was detected by western - blotting and dot - blotting 2h after irradiation
具體方法為： （ 1 ）照射后12h ，收集nih3t3細胞，用流式細胞儀檢測各組細胞的細胞周期， pcr - sscp檢測抑癌基因p16的變化； （ 2 ） nih3t3細胞照射后立即收集細胞和細胞上清，用試劑盒測量mda含量和sod 、 gsh - px的活性並觀察其變化； （ 3 ） western免疫印跡和點雜交法檢測照射2h后的各組hela細胞中基質金屬蛋白酶- 2 （ mmp - 2 ）的表達變化。 -
After transfecting the shrna based on telomerase htert into hela cells with calcium phosphate co - precipitation procedures, we detected hela cells viability by trpan blue staining method
以磷酸鈣共沉澱轉染法將shrna轉染hela細胞。于不同的時間用臺盼藍染色法檢測細胞生存能力。 -
The protein level of cyclin e increased lightly after 2gy, but it increased from 1 to 24 h after logy, level of cyclin e associated with cdk2 increased in response to logy, at the same time, there was no significant changes in the level of cdk2, while cdk2 levels remained constant in hela cells. our results indicated that the kinetics of cyclin e / cdk2 kinase activity reflected the increase in cyclin e expression levels
Cycline蛋白表達水平在zgy照射后略有增加， 1ogy照射后lh開始增加，持續到24h ，但ir后cdkz蛋白水平未發生變化，通過免疫共沉澱檢測了10gy照射后edkz與cydine結合，發現隨著照射時間的延長， cycline與cdkz結合量增多，這說明cycline蛋白水平的增加能反映cycline / cdkz的激酶活性。 -
By using gfp - cam fusion protein, we have observed the detailed dynamic redistribution of cam in hela cells during cytokinesis. in tri - polar cell model, we have studied the relationship between the distribution pattern of cam and the formation of the cleavage furrow. furthermore, we have made the study on the regulation mechanism of cam during the whole process of cytokinesis by inhibiting its activity at different cytokinesis stages
我們採用綠色熒光蛋白（ gfp ）標記技術，重點觀察了胞質分裂期hela細胞中gfp - cam的動態分佈；應用三極細胞模型研究了cam的分佈與分裂溝形成之間的關系；還通過在胞質分裂不同階段抑制cam的活性研究了cam在整個胞質分裂過程中的作用機制。 -
In this dissertation, the plasmids containing 5s promoter were transfected into hela cells, the transcription sites of rna polymerase iii and its transcripts were detected by fluorescence in situ hybridization ( fish ) to dna, rna and dna - rna, respectively
本實驗以人的hela細胞為材料，運用電擊轉染、熒光原位雜交並結合激光共聚焦顯微鏡，從dna 、 rna和dna - rna三個水平對rna聚合酶的轉錄位點及其轉錄子的分佈進行研究。 -
Effects of hpot1 overexpression on cell cycle and the apoptosis of hela cells
細胞細胞周期和凋亡的影響 -
After marking with gfp and hochest33342, we also observed the multinuclei and chromatin bridges in hela cells that were transfected with the c - terminal expressing plasmids corresponding to the human smc subunits hcap - e and hcap - c, respectively
進行rt一pcr檢測表明人集縮素smc亞基hcap一e和hcap一c的c一末端真核表達質粒在hela細胞內得到過表達。 -
Induction of apoptosis by harringtonine in hela cells
細胞凋亡的研究 -
( 3 ) establishing a new method which can quantitatively determinate telomerase activity. 1. human telomerase rna component ( htr ) gene was cloned from hela cells using gene engineering technology. the htr gene was then reverse inverted into retrovirus vector plncx and constructed an antisense recombinant plasmid plncx - atr
將htr基因插入逆轉錄病毒載體plncx中，構建了htr基因的反義重組質粒plncx - atr和正義重組質粒plncx - tr ，測序結果表明符合預期要求，為下一步實驗奠定了基礎。 -
Cloning of human oviductin cdna and its expression in hela cells
克隆及真核細胞中的表達 -
Lygdi expression in hela cells increased its sensitivity to radiation - induced apoptosis
細胞中的表達與輻射增敏研究 -
Therefore hela cells provide a good model for the study the mechbosm of llp - hsdl gene expression by
將克隆的含gre樣結構的0叫基因啟動子1 -
Using the superfect reagent, in vitro cultured hela cells were introduced with external plasmids
利用脂質體superfect介導的轉染方法將外源質粒導入體外培養的hela細胞。 -
In this dissertation, jurkat cells, hela cells and mouse spleen t lymphocytes were chosen as experimental materials to answer the question. with the aid of various techniques such as elisa, immuno - co - precipitation, indirect immunofluoresent co - localization, double - labeling immunoelectron microscopy and so on, the relationships of baf complex with nf1 / ctf and rna polymerase ii were careful observed and analyzed
本文以jurkat細胞、 hela細胞和小鼠脾t淋巴細胞為研究材料，通過酶聯免疫吸附實驗（ elisa ） 、免疫共沉澱、免疫熒光共定位和免疫電鏡雙標記等實驗，觀察和分析了baf復合物與轉錄因子nf1 ctf和rna聚合酶在基因轉錄活動中的相互聯系。 -
In hela cells, by using anti - brgl antibodies, anti - rna polymerase ii antibodies and anti - nfl / ctf antibodies, the core subunit brg1 of baf complex and rna polymerase large subunit were found well immunofluorescently co - localized, while nf1 / ctf and rna polymerase large subunit were poorly co - localized. brg1 and nf1 / ctf were also well co - localized. in order to further reveal the relationships of baf complex with nf1 / ctf and rna polymerase ii large subunit at the ultra - microscopic level, we performed the double - labeling immunoelectron microscopy experiment with hela cells
以hela細胞為材料，分別用抗brg1抗體、抗rna聚合酶抗體和抗nf1 ctf抗體進行免疫熒光共定位分析，發現baf復合物的核心亞基brg1和rna聚合酶的大亞基存在很好的熒光共定位現象， nf1 ctf和rna聚合酶的大亞基之間的共定位現象不明顯， brg1和nf1 ctf也有很好的共定位現象。 -
To investigate the silence effect of hela cells " telomerase gene expression after shrna based on human telomerase htert transfected into cells. methods : we constructed a partial double - strand dna with t7 promoter as dna template and synthesized small hairpinrna in - vitro using t7 rna polymerase
方法：根據端粒酶htert基因1573 ? 1591位的核酸序列，構建帶t7啟動子的部分雙鏈dna模板，用t7rna聚合酶體外合成短鏈shrna 。
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