hepes 中文意思是什麼

hepes 解釋
兩性離子緩沖劑
  1. After breast feeding, their descendents were fed with the same water, then killed them on the 1st, 8th, 15th, 22nd, 30th day after birth respectively and took out of the brains, stored at - 70. ( 2 ) measurement of the activity of pkc in brains : in cytoplasma ; took out the samples, cut the hippocampi into pieces and added buffer ( glucose 0. 32mol / l, hepes ph 7. 5 ), then shattered with ultrasound, centri - fuged at 27000g for ih at 4ti, moved the supernant into a new tube

    本研究以細胞信號轉導途徑為線索,分別探討了pkc 、 erk 、 nos1等信號分子及即早基因( c - fos , c - jun )在慢性鉛暴露小鼠腦細胞中的活性、蛋白表達及mrna表達的改變,以及鉛暴露小鼠記憶行為的變化,並將細胞中信號分子的改變與記憶功能改變結合進行分析以尋找一些內在規律。
  2. Anti - p21 mouse monoclonal antibody from beijing zhongshan biotechnology anti - mouse or anti - rabbit igg secondary antibody from santa cruz biotechnology ly294002 from sigma biotechnology tritonx - 100 from boehringer mannhein gmbh fluorescein isothiocyanate ( fitc ) conjugated anti - mouse igg antibody was purchased from beijing zhongshan biotechnology hepes from e. metck darmstadt methods superovulation and collection of eggs for superovulation, female kunming mice 4 - 5 week old were injected with pregnant mare serum gonadotropin ( pmsg ), and after 46 - 48 hours with human chorionic ginadotropin. ( hcg ). one - cell fertilized eggs were collected on the next day from oviduct of females

    取4一5周齡成熟雌性昆明系小白鼠,腹腔注射pmsg (孕馬血清促性腺激素) 10iu , 46一48小時后腹腔注射hcg (人絨毛膜促性腺激素) 1oiu ,將注射hcg后的雌鼠與8周以上的成熟雄鼠合籠交配,次日檢察陰栓,將查到陰栓的雌鼠處死,取輸卵管于mz培養液中,解剖鏡下撕開壺腹,釋放細胞團,然後用300林歲nil透明質酸酶消化去除顆粒細胞,口控吸管將卵細胞在m :中反復清洗,然後置於孵箱中,根據時間點收集g2期細胞。
  3. The eg cell culture media consisting of dmem medium supplemented with fbs, chicken serum, beta - mercaptoethanol, l - glutamine. hepes, chicken embryonic extract and cytokines etc. after 24 hours culture, the isolated pgcs were selectively attached on the gonadal stromall cells in the plates

    加入新鮮的eg細胞培養液(含dmem 、胎牛血清、雞血清、 -巰基乙醇、 l -谷氨酰胺、 hepes 、雞胚浸出液以及細胞因子等成分)培養24小時以後, pgcs開始部分貼附於共培養的生殖原基細胞上。
  4. In the first part, both behavioral responses and fos expression in dorsal horn induced by peripherally injected formalin were observed. animals were divided into three groups ( n = 5 ) : one of them was control with injection of vehicle ( hepes ) into the plantar aspect of the left hindpaw ; and two of them were experimental groups with injection of selective mglur, and mglur5 antagonists, cpccoet or mpep, respectively

    實驗組動物山西醫科大學的一側后腳掌分別注入20仍級碩士學位論文mg1ur 、 、 mglur 、的拮抗劑cpccoet ( 1oon二01 ) / m屍ep ( 50nmol ) ,對照組則在一側腳掌注入緩沖液hpeps (兩種拮抗劑均用hpeps溶解) 。
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