histone gene 中文意思是什麼
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5, histone acetyltransferase gene ygcns and histone deacetylase gene yrpd3 were cloned and expressed, which has laid down a foundation for studying chromosome remodeling in vitro
5 ，我們克隆並純化了酵母中的組蛋白乙酞基轉移酶基因gcns和去乙酞化酶基因rpd3 。 -
Epigenetic modification of the genome ensures proper gene activation during development and involves : genome methylation changes ; the assembly of histon and histone variants into nucleosomes and remodeling of other chromatin - associated proteins such as linker histones, polycomb group, nuclear scaffold protein, and transcription factors
這些染色質的再程序化包括dna的甲基化和核小體組蛋白的乙酰化等。組蛋白乙酰化在染色質結構調節、 dna復制、基因轉錄、細胞分化及癌細胞發育都有研究，但在克隆動物中卻是一個有待深入探索的領域。 -
However, it is difficult to relate the histone acetylation level with the gene expression level. we indicate, although histone acetylation acts to enhance the access of transcription - associated protein to dna, the activation of gene expression only need containing some acetylation level, but the hyperacetylation and underacetylation would effect the gene expression, so as to the abnormal development
而在克隆牛中組蛋白乙酰化水平雖然有所降低，但如果這種降低仍然為基因的表達提供了足夠的基礎，這就對基因的正常表達造不成很大影響；但如果組蛋白乙酰化水平過高或過低，可能要影響到其他因子的作用，從而影響了基因表達的調節，最終導致生物個體發育異常。 -
Human elongator complex serves a role in chromatin remodeling through modifying the lysine residues of histone h3 and h4 during transcription elongation in higher cells. the main results and conclusions are as followings. 1 using depletion strain of yeast as material, we have established a system for complementation test and gene expression analysis of human elongator complex
4 、利用點突變和plasmidshuffle等技術構建了含h3k14一r 、 h4ks ~ r單突變和h3k14一側h4ks一r雙突變的e1p3乙菌株，通過功能互補實驗分析少凡p3對突變株的補償功能，證實酵母elongator可對h314k / h4sk進行乙酞化修飾。 -
Combined with the results of histone deactylase inhibitor, it is suggested that histone acetylation plays an important role in hsp gene transcription regulation
結合以上去乙酞化酶抑制劑的實驗結果，我們認為組蛋白乙酞化修飾參與了熱休克基因的表達調控。 -
We suggest : ( 1 ) histone must maintain some acetylation level whether the associating - gene is activated or inactivated ; ( 2 ) acetylation and deacetylation are global in these genes " chromosomal domains ; ( 3 ) but uneven ; ( 4 ) and the level will change according to the development stage
通過我們的實驗，我們認為無論基因表達與否，都首先在其基因組上維持了一定的組蛋白乙酰化水平，這種乙酰化水平同時也是一個整體的概念，而且在不同的基因區域上，組蛋白乙酰化水平是有一定的波動性，另外這種乙酰化水平可以隨著發育階段的不同根據基因表達的要求發生變化。 -
Arginine rich human histone h3 is a kind of basic nucleosome protein. in the medium of physiological condition, by electrostatic interaction, histone h3 which arginine impart to it a positive charge binds to dna which phosphate groups impart to it a negative charge. in this paper, in order to establish a new technology of transfection, the functional domains la and ii gene segments of pea were lined with histone h3 gene segment, then a recombinant fusion protein was constructed which serves as c arrier for transfer of dna via receptor - mediated endocytosis
本實驗在已成功獲得的pea功能區a ，功能區基因片段與人組蛋白h3基因片段的克隆菌株的基礎上，構建原核表達載體，將克隆質粒pmd - 18t - pea經nco ， mlu雙酶切，回收酶切產物；將克隆質粒pmd - 18t - h3經mlu ， xho雙酶切，回收酶切產物。 -
4, compared the influence of two histone deacetylase inhibitors on the hsp70 gene transcription, after changing the time and the concentration of the inhibitors, we found the mechanism of the two histone deaceylase inhibitors is different
4 ，通過比較改變餵食時間和餵食濃度后兩種去乙酞化酶抑制劑與熱休克基因表達水平之間的關系，我們發現tsa與丁酸鹽抑制去乙酞化酶的作用機制可能是不同的。 -
2, the hsp gene transcription was quantitatively determined by rt - pcr. based on this result, it is concluded that the change of acetylation level at the loci of hsp, mediated by histone deacetylase inhibitors, exerts important functions in hsp gene transcription. 3, after immunolabeling with anti acelated - lysine monoclonal antibody on the polytene chromosomes of heat shocked flies, fluorescence signals were detected at the hsp loci
2 ，利用rt - pcr技術，對去乙酰化酶抑制劑處理后的果蠅的熱休克基因的表達水平進行檢測，結果表明經組蛋白去乙酰化酶抑制劑處理后的果蠅幼蟲，其熱休克基因的表達高於基礎水平，也就是說去乙酰化酶抑制劑誘導了熱休克基因的表達。
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