immunoblotting 中文意思是什麼
immunoblotting
解釋
免疫墨點-
Linearized the expression vector ppic9k - p including the truncated mutant pokeweed antiviral protein ( pap ) gene by restriction endonuclease sal i and transformed it electrically into pichia pastoris gs115. mut + recombinants were selected by pcr and high yield mut + recombinant was picked out by double film immunoblotting
本研究將含有n末端信號肽和c末端毒性區缺失的pap基因的表達載體ppic9k - p用sali酶切線性化后，通過電擊轉化整合p . pastorisgs115菌株細胞中。 -
Westen immunoblotting with dl antibody and an approach detecting thylakiod phosphorylated proteins were used to check dl proteins of different forms, quantitive scranner to count abundances of proteins and fluorometry to detect the maxmin fluorescence ( fm ) of chlorophylls and influorescence of proteins
實驗結果顯示： （ 1 ）光抑制處理使葉綠素a和葉綠素b發射熒光明顯下降； （ 2 ）光抑制處理時類囊體膜蛋白內源熒光並不發生明顯變化。 -
The mutants were transfected into pc 12 cells respectively to probed the ability respondence to gdnf stimulation and the interaction with ret by immunoprecipitation, c - ret phosphorylation and immunoblotting assays. the main results are as follows : 1. expression and purification of recombinant rat gfral protein to obtain recombinant gfral and study its biological activity, the cdna encoding the mature rat gfral was isolated using rt - pcr with total rna extracted from newborn s
純化和復性后的重組gdnf蛋白，可顯著增強pc12一gfral一ret工程細胞的存活和分化;對pc12一ret工程細胞沒有任何作用;對pc12一gfral的存活和分化作用11中英文摘要顯著強于對pc12一ret的作用，但也顯著低於對pc12一gfral一ret細胞的作用。
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