immunocytochemistry 中文意思是什麼

P - liquid scintillation counting was used for analysis of erk and immunocytochemistry was used for the analysis of c - jun. all the data were analyzed with statistical treatment. the results were showed as follows : 1

分別做p液閃計數觀察erk的活性表達，做westernblot觀察jnk的表達量變化，免疫組化觀察cjun陽性信號的表達，並進行統計學處理，結果如下： 1

Nogo - 66 receptor, ngr, cloned in 2001, is a leucine - rich - repeat glycophosphatidylinositol - anchored membrane protein which mediates nogo - 66 inhibition of axonal outgrowth. both the long acidic amino - terminal domain and the nogo - 66 fragment have strong neurite growth inhibitory activity suggest that nogo - a has at least two inhibitory domains. northern blot, in situ hybridization, western blot and immunocytochemistry analyses show that in addition to oligodendrocytes, nogo - a mrna and nogo - a protein are also expressed in neurons in developing and adult brain and spinal cord, nogo - a is also found in peripheral organs such as heart and testis

Northernblot 、原位雜交、 westernblot和免疫組化結果證明： nogo amrna和nogo蛋白除了在cns的寡突膠質細胞中表達，還表達于發育階段和成年的腦、脊髓和外周神經節的某些神經元中，在外周組織如睪丸和心臟也有表達； nogoe在cns和pns以及多種外周組織中有廣泛分佈； nogo （除表達于腦和心臟外，在骨骼肌中有較高表達。

Mdr1 express product p - glycoprotein was detected by immunocytochemical method and flowcytometry. the cytotoxicity and multidrug resistance reversion effect of tea polyphenol was examined by mtt assay in mcf - 7 and mcf - 7 adr carcinoma cell lines, and compared with pgp inhibitor quinidine. the pgp expression of mcf - 7 adr was strongly positive, the positive rate was 15 % ; the pgp expression of mcf - 7 was negative, the positive rate was 1. 8 %. ic50 of tea polyphenol to mcf - 7 and mcf - 7 adr is 115. 2g ml and 207. 6g ml respectively. ic50 of quinidine to mcf - 7 and mcf - 7 adr is 129. 8mol l 42. 1g ml and 94. 1mol l 30. 5g ml respectively. tea polyphenol and quinidine changed little toxicity of adriamycin to mcf - 7, but tea polyphenol and quinidine improved the sensitivity of mcf - 7adr to adriamycin significantly. immunocytochemistry and flow cytometry can detect p - glycoprotein expression level qualitatively and quantitatively. tea polyphenol is not only an anti - tumor agent, but also a multidrug resistant modulator similar as quinidine. tea polyphenol is advantageous for its little toxicity in tumor treatment

用免疫組化法和流式細胞儀對腫瘤細胞系mcf - 7和mcf - 7 adr的p -糖蛋白表達水平進行定性定量研究。用噻唑藍比色法mtt研究茶多酚的細胞毒性及其對耐藥性的逆轉作用，並與pgp抑制劑奎尼定進行了比較。免疫組化法檢測p -糖蛋白表達水平， mcf - 7 adr呈強陽性，而mcf - 7呈陰性流式細胞儀定量檢測結果mcf - 7 adr細胞系細胞陽性率為15 % ， mcf - 7細胞系細胞陽性率為1 . 8 % 。

Injected group, 0. 1 % saccharin ( 1. 5 ~ 2ml / rat, in 5min ) intraoral infused group and cta group. the expression of endogenous leucin - enkephalin ( lek ) in the rat brain was observed and 5 parts of the thalamus including laterodorsal thalamic nucleus ( ld ), lateral part of mediodorsal thalamic nucleus ( mdl ), ventroposterolateral thalamic nucleus ( vpl ), ventroposteromedial thalamic nucleus ( vpm ) and reticular thalamic nucleus ( rt ) were comparatively researched before and after the acquisition of cta applying lek - immunocytochemistry. in behavioral experiment, 18 adult male sd rats were divided into normal cta group ( control ) and 2 naloxone i. p

為探討cta形成過程中enk的作用，本實驗用成年雄性sd大鼠35隻，分為空白對照組、生理鹽水（ 2體重）腹腔注射組、 0 . 15mlicl溶液（ 2體重）腹腔注射組、 0 . 1糖精溶液口腔灌流組（ 1 . 5 - 2ml只， 5min ）和cta建立組，採用免疫細胞化學方法，觀察了亮腦啡肽（ lek ）陽性神經元在大鼠腦內的分佈情況，並比較了各組大鼠丘腦外側背核（ ld ） 、丘腦內側背核外側部（ mdl ） 、丘腦腹后外側核（ vpl ） 、丘腦腹后內側核（ vpm ）以及丘腦網狀核（ rt ）等5個腦區內lek表達水平的差異；另外將成年雄性sd大鼠18隻，分為正常cta建立組以及在cta建立前或cta建立后阿片受體拮抗劑納洛酮（ 2mg kg體重）腹腔注射組，對內源性阿片樣物質對于cta建立和保持的影響進行了行為學研究。

After the recombinant plasmid pcdna3. 1 / ts87 was identified by digestion of hindlll and bamh i, it transformed into cos7 by lipofectamine. expression product was identified by immunohistochemical method, sds - page and western - blot. the immunocytochemistry result has shown that specific brown - staining grains were found in the cytoplasm of cells transformed by recombinant plasmid versus not seen in cells transformed by pcdna3. 1 or normal cells ; the sds - page result has revealed that a band about 3 8kb was found in cell lysis transformed by recombinant plasmid versus not in cells transformed by pcdnas. l or normal cells ; the western - blot result has showed that only the band about 38kd was recognized by sera from rabbit infected by t. s artificially and sera from rabbit immunized with soluble antigen of t. s and with protein expressed by ts87 gene and by a monoclonal antibody of t. s

通過細胞的免疫組化，細胞裂解物的sds - page電泳， westem - blot分析檢測目的基因的表達情況。免疫組化結果顯示：重組質粒轉染的細胞質中有棕褐色顆粒，而空載體轉染細胞及正常細胞無此現象；細胞裂解物sds - page電泳結果顯示：只有重組質粒轉染的細胞在約38kd處有明顯的蛋白帶，這與理論計算的ts87基因表達蛋白的分子量為38kd基本一致； western - blot分析結果顯示：約38kd的蛋白帶能夠分別被旋毛蟲感染兔血清，成蟲蟲體可溶性抗原免疫兔血清， ts87基因原核表達蛋白免疫兔血清（ ts87血清）以及一株具保護性的旋毛蟲單抗特異識別。

The present results indicated that the paraventricular nucleus of the hypothalamus and the supraoptic nucleus might have important roles in neuroimmunomodulation. 2. following lps or seb was administered intraperitoneally, the expression of pcna of splenic cells and il - 1 receptor type i in pvn and son were observed by using immunocytochemistry in the mice. double fluorescent labeling technique was used to determine the relationship of il - 1 receptor type i co - expressions with arginine vasopressin or oxytocin

二、小鼠腹腔內給予細菌內毒素lps或腸毒素seb ，用免疫組織化學方法觀察了脾臟核增殖抗體及下丘腦室旁核和視上核中1型il 1受體的表達，並採用雙標記技術觀察了1型il刁受體陽性神經元和加壓素及催產素表達的關系。

Day 4 embryoid body ( 4deb ) cells were derived from es cells and then induced with mbmec - cm into hematopoietic precursor cells. immunocytochemistry staining and flow cytometry were adopted to observe the antigen expressions. rt - pcr method was used to detect the expressions of hematopoietic genes

在誘導小鼠es細胞向造血細胞分化的實驗中，我們採用先將小鼠胚胎幹細胞形成4天擬胚體（ day4embryoidbodies ， 4debs ） ，再用骨髓內皮細胞條件培養液誘導4天擬胚體細胞生成造血干祖細胞。

In the laboratory experiment part, human peripheral blood, cultured cells and icr mice were study objects. the changes of mitotic chromosome numbers were measured by human metaphase chromosome counts and statistic analyzed used x2 - test. the changes of meiotic chromosome numbers were measured by mice one - cell zygote chromosome counts and statistic analyzed usedx2 - test. the effects of low dose ionizing radiation on the expression of topoisomerase ii were measured by immunocytochemistry, western blot and rt - pcr

流行病學結果顯示長期小劑量輻射接觸與染色體不分離呈正相關，為進一步在細胞遺傳學和分子生物學方面研究小劑量電離輻射與染色體不分離關系及其機制，本課題第二部分以外周血、培養細胞、 icr小鼠為研究對象，用外周血染色體計數和單細胞受精卵染色體計數的方法研究小劑量輻射和拓撲異構酶復旦大學2000級博士生學位論文11a抑制劑及其二者的協同效應對有絲分裂和減數分裂染色體不分離的影響，用免疫細胞化學染色、 westernblot 、 rt pcr等方法研究了電離輻射引起拓撲異構酶a表達變化。

M ethods we successfully expanded human embryonic brain - derived nsc into spheres with mitogens. the nsc were identified by immunocytochemistry method, brdu labeling and cell cloning were used to observe the proliferation ability of nsc. pdgf x t3 were separately used to induce the differetiation of nsc

方法：本實驗以胎齡為10 - 12周的人大腦皮質為材料，在體外成功誘導擴增nsc ，用免疫細胞化學方法鑒定nsc ，用brdu標記和細胞克隆分析觀察了nsc的增殖能力。

Result the result of the first part : 1. immunocytochemistry reveals that breast cancer cells of t47d and mda - mb - 231 have positive reaction. positive particles position cell membrance and cell plasma respectively

結果：第一部分結果：天津醫科大學碩士學位論文中文摘要1 .免疫組化顯示: t47d陽性，陽性反應物定位於胞膜和胞漿。

From the point of morphology and immunocytochemistry, it is proved that it is reasonable to analysis the follicular steroidogenesis of the rana quadranus by using the two - cell type model

從形態學和免疫細胞化學的角度證明，用兩類細胞模型解釋卵巢類固醇激素的合成與分泌是合理的。

We used immunocytochemistry to explore the expression of il - 2r

免疫細胞化學反應：檢測ilzr的表達； 5

The fetal liver stem cells were isolated by collagenase digestion, gravity sedimentation and density gradient centrifugation, identified by immunocytochemistry and evaluated by flow cytometry for their proliferation condition

採用膠原酶消化、重力沉降及密度梯度離心方法分離人胎肝幹細胞，通過免疫細胞化學方法對其進行初步鑒定，以及應用流式細胞儀等對其生長狀況進行評估。

We detected the transcription and translation change of different thrs genes by semiquantitative reverse transcription - polymerse chain reaction ( rt - pcr ) analysisjelisa respectively. at same time the differentiated cell were identified by immunocytochemistry method. results ( l ) nsc induced by mitogens were nestin - positive and incorporated by brdu

以pdgf 、 t _ 3對nsc進行誘導分化，並分別用rt - pcr半定量法、 elisa法檢測nsc在不同誘導因子作用下分化前後thr各亞型在轉錄與翻譯水平上的表達變化，用免疫細胞化學方法鑒定分化后的細胞類型。

In the second trial, this modified discontinuous percoll gradient centrifugation method was introduced to isolate spermatids from the semen of fifteen male infertile patients. then the effect was identified by wright - giemsa stain, flow cytometry analysis, immunocytochemistry and fluorescence in situ hybridization ( fish ). similary, the 22 % percoll fraction contained mostly haploid cells [ ( 91. 85 ? 5. 18 ) % ] ( p < 0. 005 ) and the mean density in this fraction was ( 1. 010 ? 0. 786 ) x 105 / ml

C法，對15例各種類型不育患者的精液細胞進行分離，並利用瑞姬染色法、流式細胞術、免疫細胞化學和熒光原位雜交oisffi等方法，從細胞形態特徵、 dna倍體、細胞表面標i己與分化抗原，以及原位雜交信號的數目和位置結合細胞核特有的形態等方面加以鑒定。

Two hours later, according to the protocol of the sabc kit of boster company, an immunocytochemistry was done to examine whether there is neuron - specific proteins in cytoplasm

5小時後用含2 dmso的誘導液處理5個小時。取誘導10小時后的細胞，參照博士德公司的sabc試劑盒操作方法進行免疫細胞化學實驗。

Using the double - labeled methods of visceral nociceptive stimulation induced by formalin combined with immunocytochemistry, it was observed that the cb positive neurons expressing fos protein distributed in the related nuclei of brainstem in the rat. under microscope it was showed that there were a various numbers of fos / cb double - labeled neurons, besides fos and cb single - labeled neurons, in nts, vlm, lc, lpb and vlpag

第四軍醫大學碩士學位論文給予內臟傷害性刺激並結合免疫組化雙重標記染色，觀察了大鼠腦干相關核團內含cb並表達fos的神經元分佈狀況，結果顯示：在nts 、 vlm 、 lc 、 pb和vipag等核團內除下。

There were significant difference between psd group and the two control groups ( p < 0. 05 ), no significant difference between the two control groups. 4. immunocytochemistry : the c - jun positive cell nuclei appeared brownish granules

正常對照組和快眼動睡眠剝奪對照組海馬各區均未見明顯的c jun陽性信號表達。

Two days later ( that is, these cells had been cultured for ten days in vitro from isolation ) these cells were collected and immunocytochemistry was performed by anti - nestin to identify nscs and by anti - brdu to evaluate nscs " proliferating capability

3 . 4ap25一35和ap25一35 +雌激素作用后細胞增殖能力檢測對照組神經幹細胞brdu陽性細胞率約為50 % ;雌激素保護組及ap毒性組brdu幾乎未見陽性細胞。