immunoprecipitation 中文意思是什麼
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However, it is necessary to acquire the antibody or the antiserum, which could specially react with the expression protein of die objective gene transferred into the transgenic plant according to the characteristics of high homology and immune cross - reaction among plant ferritin, using the special immune serum of pea ferritin, the content of plant ferritin could be detected for studing the ferritin expression of transgenic plant by the technique of immunoassay such as immunoprecipitation, eljsa and western blotting
利用免疫檢測技術進行植物轉基因的表達檢測是一種簡單、靈敏、快速、可靠的方法，但其前提條件是要有與轉基因植物目的基因表達的蛋白質發生特異性免疫反應的抗體或抗血清。根據植物鐵蛋白之間有高度同源性和交叉免疫反應的特性，利用特異性的豌豆鐵蛋白抗血清，就可通過免疫沉澱、 elisa或western雜交等免疫檢測方法進行植物鐵蛋白含量等的檢測，從而更好地進行轉基因方面的研究。 -
This study demonstrated that the arabidopsis f - box protein coil associated with atcul1, atrbxl and skpl - like proteins askl and ask2 to assemble scfcoil ubiquitin ligase complexes. also, we found that the atcull component of scfcoil complexes contained two species including atcull and modified atcull. ( 2 ) we found that coil assembled to two separate scfcoil complexes with either askl or ask2 through immunoprecipitation analysis with plant expressing myc - tagged version of ask2
用表達融合蛋白myc - ask2的擬南芥為材料，以- myc抗體進行免疫共沉澱分析發現， myc - ask2蛋白可以與coi1蛋白一起免疫共沉澱，但是不能與ask1蛋白免疫共沉澱，表明coi1蛋白與ask2蛋白，但是不能同時與ask1結合形成scf ~ （ coi1 ）復合體。 -
The protein product of meq gene was highly expressed in the nuclei of recombinant baculovirus infected sf9 cells when using an anti - meq monoclonal antibody ( mcab ) 23b46 to run the immunofluorescence assay ( fa ) ; the expression quantity and if staining patterns differed with different times post - infection ( pi ). the results of western blotting and immunoprecipitation test showed there were two specific bands around 60 kd. the results of the study demonstrated that the baculovirus / insect cell system is effective to be used to express nuclear protein of virus
結果發現:本表達系統產生的meq蛋白可被重組痘病毒表達的meq制備的單抗23b46所識別;在感染細胞中， meq蛋白僅局限於細胞核內，而且隨著感染后（ pi ）時間的增加，具有從核質向核仁和核膜轉移的趨向; w已stemblot和免疫沉澱試驗均證實重組桿狀病毒感染細胞裂解物中出現有兩條大小約為60kd的特異帶。 -
Herein, we used cloning, sequencing, northern blotting, in situ hybridization, in vitro translation and co - immunoprecipitation, aimed to investigate the expression of wnk genes and the function of wnk4 kinase
本實驗應用克隆、測序、 northern印跡雜交、原位雜交、體外翻譯和免疫共沉澱技術，旨在研究wnk基因的表達情況及wnk4激酶的功能。 -
And also for the establishment of the technique, we were interested in examining whether rad18 plays a role in this ho - induced dsb repair in s. cerevisiae. to measure the association of radls with dsb, we used the chromatin immunoprecipitation ( chip )
但是具體這一修復復合物的泛素化底物蛋白是什麼，它們又具體結合於什麼樣性質的dna損傷部分，目前並不清楚，而這兩點是研究泛素系統在dna修復中的作用的關鍵。 -
Sheshberadaran h, et al. antigenic relationship between hantaan viruses analysed by immunoprecipitation. j gen virol, 1988, 69 : 2645
梁米芳，等.漢坦病毒s基因在大腸桿菌中的表達及其初步應用.中華實驗與臨床病毒學雜志， 1993 ， 7 : 225 -
The mutants were transfected into pc 12 cells respectively to probed the ability respondence to gdnf stimulation and the interaction with ret by immunoprecipitation, c - ret phosphorylation and immunoblotting assays. the main results are as follows : 1. expression and purification of recombinant rat gfral protein to obtain recombinant gfral and study its biological activity, the cdna encoding the mature rat gfral was isolated using rt - pcr with total rna extracted from newborn s
純化和復性后的重組gdnf蛋白，可顯著增強pc12一gfral一ret工程細胞的存活和分化;對pc12一ret工程細胞沒有任何作用;對pc12一gfral的存活和分化作用11中英文摘要顯著強于對pc12一ret的作用，但也顯著低於對pc12一gfral一ret細胞的作用。 -
Co - immunoprecipitation kit and multiple tissue membranes were from clontech company. 2
Northern印跡雜交膜和eo一irrununopreeipitationkit購自clonteeh公司。 -
Otherwise ; we have used immunoprecipitation and im - munofluores cence microscope technology to study the interaction with doc - 1r and cdk2 proteins under physiological condition in cells, we attempt to find the evidence of interaction with doc - 1r and cdk2 proteins, which will offer experiment basis for understanding the accurate role for doc - 1r gene in cell cycle s regulation
另外我們採用免疫共沉澱技術和免疫熒光顯微技術觀察在生理條件下細胞內doc - 1r和cdk2蛋白間相互作用的情況，試圖找到細胞內doc - 1r與cdk2蛋白結合的證據，為準確掌握doc - 1r基因在細胞周期調控中的作用提供實驗依據。
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