in situ pretreatment 中文意思是什麼
in situ pretreatment
解釋
原位預處理- in : adv 1 朝里，向內，在內。 A coat with a furry side in有皮裡子的外衣。 Come in please 請進來。 The ...
- situ : 西楚
- pretreatment : 初步加工
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The organization cuts into slices and examines by the in situ pcr, drip protease k 20 ( xl with loomg / ml to digest respectively in pretreatment, increase with normal position positive cell account for total ratio of cell, according to the positive standard cells > 75 %, confirm the lightest digestion time, studying the influence and relationship of different fixation time with protease digesting each other, detecting the mn genotype of the organize slices at the same time
石蠟切片進行原位pcr檢，預處理分別滴加loom歲血的蛋白酶k20閃消化，以原位擴增顯色后陽性細胞占總細胞的比值> 75 %為標準，確定最適消化時間『 ， ，研究不同固定時間與蛋白酶消化的相互影響和關系，同時檢測石蠟切片的mn基因型。 -
The key stage of fabricating gene chip is pretreatment of glass surface including the processes of nh3h2o treatment, aminosilane treatment and aldehyde treatment. the pretreatment can grow active group that can bind probe effectively on the surface of glass slide. as a result, the actively treated glass slide can suit for fabricating in - situ synthesis high density gene chips
基因晶元制備技術的關鍵步驟是玻片表面預處理，即對玻片表面進行羥基化、氨基化和醛基化處理，使表面生長的活性基團能有效固定寡核苷酸探針，以滿足原位合成高密度基因晶元對玻片的要求。
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