in-situ hybridization 中文意思是什麼

in-situ hybridization 解釋
原位雜交
  • in : adv 1 朝里，向內，在內。 A coat with a furry side in有皮裡子的外衣。 Come in please 請進來。 The ...
  • situ : 西楚
  • hybridization : 混成作用
  1. The open reading frame of tsarg2 was obtained from human testis cdna library by pcr. using in situ hybridization on tissue section of human testis. we preliminarily studied the expression and function of tsarg2

    從小鼠睪丸cdv文庫中分離出該基因完整閱讀框cdna ， ； hu基因的cdna全長為1088hp ，包含6個外顯于，基因組跨越9
  2. Whole mount in situ hybridization showed that amphihmgb transcripts were present ubiquitously in the early embryos from zygote to neurula, but they decreased gradually in mesoderm and endoderm and neural tube with development, and became restricted to epidermal cells in the hatched larva. it appear s that hmgb plays a role in the mitosis and differentiation of embryonic cells. another amphioxus cdna, amphiubf80, encoding ubiquitin / ribosomal fused protein s27a, was isolated from the gut cdna library of b. belcheri tsingtauense

    S004cdna序列編碼一個156個氨基酸殘基組成的蛋白質，與人的泛素蛋白ubf80具有較高的氨基酸序列同源性（ 145 / 156同源） ，且該蛋白具有泛素蛋白ubf80所共有的兩個結構域，即由72個氨基酸組成的泛素介導的蛋白降解結構域和由48個氨基酸組成的核糖體蛋白s27a結構域。
  3. Clinical applications of fluorescence in situ hybridization

    技術的臨床應用研究
  4. The more accurate localization of 3galt - l mrna expression in mouse brain as studied by in situ hybridization was in good agreement with the general expression pattern seen with northern blot hybridization. in newborn mice, the dense granular layer and the purkinje cell showed a strong signal

    我們發現在小腦中1型鏈結構的變化與邵galt一1的表達變化基本一致:在新生鼠小腦中， 1型鏈結構在蒲肯野細胞和顆粒層細胞中都有表達，而在成年鼠小腦中， 1型鏈結構僅在蒲肯野細胞中表達。
  5. Nogo - 66 receptor, ngr, cloned in 2001, is a leucine - rich - repeat glycophosphatidylinositol - anchored membrane protein which mediates nogo - 66 inhibition of axonal outgrowth. both the long acidic amino - terminal domain and the nogo - 66 fragment have strong neurite growth inhibitory activity suggest that nogo - a has at least two inhibitory domains. northern blot, in situ hybridization, western blot and immunocytochemistry analyses show that in addition to oligodendrocytes, nogo - a mrna and nogo - a protein are also expressed in neurons in developing and adult brain and spinal cord, nogo - a is also found in peripheral organs such as heart and testis

    Northernblot 、原位雜交、 westernblot和免疫組化結果證明： nogo amrna和nogo蛋白除了在cns的寡突膠質細胞中表達，還表達于發育階段和成年的腦、脊髓和外周神經節的某些神經元中，在外周組織如睪丸和心臟也有表達； nogoe在cns和pns以及多種外周組織中有廣泛分佈； nogo （除表達于腦和心臟外，在骨骼肌中有較高表達。
  6. 5 the expression of gm - csf mrna in the nscs was detected by in situ hybridization

    5應用原位雜交技術觀察神經一幹細胞gm一csfmrna表達。
  7. Half animals were perfused with paraformaldehyte following normal serum after test innnediatly, then removed the brain and fixed for 24 hours with paraformaldehyte, paraffin sections were prepared at 3um, histochemical staining for counting ce11 pro1iferating ratio and in situ hybridization for calculating integra1 scores of stem cell factor inrn positive signals

    結果如下： 1增殖細胞的觀察經72h的快眼動睡眠剝奪，實驗組大鼠海馬齒狀回區brdu與p皿a （ proliferatingcellneuclearantigen ， pma ）陽性標記細胞數顯著增加。
  8. The aim of this study was to examine caveolin - 1 expression and regulation in mouse uterus during early pregnancy, pseudopregnancy, delayed implantation, artificial decidualizarjon and hormonal treatment by in situ hybridization and immunohistochemistry

    在妊娠第1天的子宮肌層中，可以檢測到小窩蛋白- 1mrna和蛋白的表達。在第3 - 4天的子宮基質中可以檢測到小窩蛋白- 1mrna和蛋白的表達。
  9. The aim of this study was to examine ppar5 expression in rat and mouse uterus during early pregnancy, pseudopregnancy, delayed implanation, artificial decidualization and regulation by steroid hormone treatment by in situ hybridization and inununohistochemisny the expression of ppar gene in preimplanation embryo was also determined by rt - pcr

    本實驗以大鼠和小鼠為材料，利用原位雜交和免疫組化方法檢測了ppar基因在早期妊娠子宮中的表達，並利用假孕、延遲著床、人工蛻膜化及激素處理等模型研究ppar基因在子宮中的表達與調節。
  10. Pag, hippocampus etc. ) and inhibiting ca2 + currents hi rat hippocampal and dorsal root ganglion neurons. immunohistochemical and in situ hybridization histochemical studies have demonstrated orl1 receptor, cannabinoid receptor and their ligands are densely distributed in the superficial doral hom of the spinal cord

    免疫組織化學和原位分子雜交實驗結果顯示這兩類受體及其配體在脊髓背角淺層均有大量的分佈（ antonetal . ， 1996 ; farquhar - smithetal . ， 2000 ） 。
  11. The localization and expression of prolactin receptor from inner mongolia alpas cashmere goat were studied by sacpic staining, in situ hybridization and western blotting. samples of skin were taken at interval three months from birth, three months old, six months old, nine months old, ten months old or twelve months old, which correspond to summer, autumn, winter and spring. paraffin sections of hair follicles were stained with sacpic staining and in situ hybridization. the protein of prolatin receptor is abstracted from samples of skin in order to study on expression of prolactin receptor. there are prolactin receptors in outer root sheath, dermal papilla and inner root sheath. the growth of primary follicle is continuous

    本實驗從絨山羊出生后每隔三個月采一次皮樣，共分為4個月齡（ 3 、 6 、 9 、 10或12 ）段，通過製作石蠟切片，原位雜交、染色，並提取皮樣蛋白做westernblotting等實驗研究方法，研究了催乳素受體mrna催乳素受體在不同生長季節的內蒙古阿爾巴斯白絨山羊皮膚毛囊中的定位與表達，染色結果發現阿爾巴斯白絨山羊初級毛囊全年持續生長，次級毛囊的生長情況隨季節而變化，秋冬季生長旺盛，夏季生長緩慢與絨毛生成規律呈正相關。
  12. Detection of chromosome aberration of patients with lymphoma by in situ hybridization and cytogenetic technology

    應用原位雜交和細胞遺傳學方法檢測淋巴瘤患者的染色體畸變
  13. The expressed product was lysised with supersonic wave and purified by sds - page. elisa analysis revealed that the antigenicity of the vpi protein has been detected. the forth part - detection of aev - nh937 strain by in situ hybridization ( 1sh ) - probe was labelled with digoxigenin ( dig ). then the probe hybrid with 5d, 10d, and 20d postinfection brain tissue of chicken. the results of the ish showed that the positive signal was found in 3 cases, while control group was negative. there has been a reasonable correlatien between this method and other detection test

    經超聲波裂解，用尿素溶解包涵體，電泳純化后，利用elisa檢測vp _ 1外殼蛋白，表明具有一定抗原性；第四部分：應用原位雜交檢測aevi用dig標記的探針與sd 、 10d 、 20d的攻毒雞腦組織雜交，來檢測那v的rna 。
  14. 6, we used gcn5 and rpd3 genes as probes to detect the homologous sequences in drosophila melanogaster by fluorescence in situ hybridization ( fish ). this work has provided useful information for the localization and cloning of related histone acetyltransferase and histone deacetylase genes in drosophila melanogaster

    6 ，利用己獲得的酵母gcns和rpd3基因為探針，對果蠅多線染色體進行原位雜交實驗，試圖找出與gcns和rpd3基因同源的基因片段。為今後克隆和分離果蠅中與乙酞化和去乙酚化相關的基因奠定基礎。
  15. Study of gene expression of p16 with in situ hybridization technique

    16基因在小鼠胚胎發育過程中的表達
  16. $ mx # ( 2bo2 ) ffr $ $ htet # $ nq9i & kk $ 4n8 $ $ 4ta scf mana $ ismffs whether s1eep deprivation cou1d induce neuron pro1iferation has not got definite conclusion so far. in the present research, we performed small pedestal s1eep deprivation method to remove rapid eye movement s1eep ( rems ). we observed the neuron pro1iferation and differential in immunohistochemistry and doub1e 1abeling iariunohistochemistry staining, and the expression of stem ce11 factor mrna in situ hybridization technique after s1eep deprivation in rat hippocampus

    本研究應用細胞增殖標記brdu cna免疫組織化學方法結合雙重標記兔疫組織化學技術，觀察睡眠剝奪對成熟大鼠腦內神經元增殖及分化的影響，應用原位雜交技術觀察了睡眠剝奪大鼠海馬神經細胞scf基因inrna表達的變化，對睡眠剝奪引起海馬神經細胞增殖的分子機製作了初步探討。
  17. Herein, we used cloning, sequencing, northern blotting, in situ hybridization, in vitro translation and co - immunoprecipitation, aimed to investigate the expression of wnk genes and the function of wnk4 kinase

    本實驗應用克隆、測序、 northern印跡雜交、原位雜交、體外翻譯和免疫共沉澱技術，旨在研究wnk基因的表達情況及wnk4激酶的功能。
  18. Application of fluorescence in situ hybridization in diagnosis of down ' s syndrome

    用熒光原位雜交技術檢測唐氏綜合征
  19. The application of fluorescent in situ hybridization in preimplantation genetic diagnosis

    熒光原位雜交技術在胚胎植入前遺傳學診斷中的應用
  20. Protocol of dot blot and in situ hybridization for white spot virus

    對蝦白斑病毒斑點雜交和原位雜交檢測操作規程
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