induced t cell 中文意思是什麼
induced t cell
解釋
誘導性 t 細胞-
Indeed, although gm - csf - stirnulated b7 - deficient dc progenitors induced only low levels of t cell priining and prolonged allograft survival, they failed to induce indefmite cardiac allogr8ft sed
4mm的套管在術中安裝于受者頸內動脈，接通移植心臟與受體小鼠頸部的血管，置移植心臟于受體的頸部。 -
These results suggested that axud1 protein functioned in a different way in the expression of cell cycle and apoptosis related proteins induced by tgf - 1 in tumor cells. 5. axudl cdna fragment was cloned into procaryotic expression vector pqe30 and t
5 . axudlcdna片段成功地克隆入原核表達載體pqe30 ，並用iptg誘導出axudi融合蛋白的表達，這為進一步純化axudi蛋白，研究axudi蛋白的免疫組織化學定位和結構功能奠定了基礎。 -
On the bases of designing a primer pair, we obtained the coding domain sequence of rbp by pcr from cdna library. then the gene was cloned into pgem - t vector, the dna sequencing showed that the cloned gene was in agreement with the reported sequence. then the tageted gene of rbp was further subcloned into a procaryotic expression vector pbv220 and constructed recombinant plasmids was named pbv220 - rbp. in order to expresse rbp in procaryotic cell efficiently, the recombinant plasmids was introduced into e. colidhs a straints. expression of rbp was induced by temperature induction
本實驗在合成該蛋白基因上下游引物的基礎上，利用pcr技術，從人睪丸cdna庫中釣取目的基因，並克隆于pgem - t載體中，經序列測定證明與文獻報道基本一致，再將目的基因從重組克隆質粒中亞克隆于pbv220原核表達載體中，轉化宿主菌，經溫度誘導后，進行sds - page電泳分析，發現在約21kd位置上出現了一條明顯的蛋白帶，與預期相符。 -
In this study, pichia pastoris system had been utilized for expression of fmdv 2c3abc gene which aimed for establishing a sensitive and specific molecular dignosis method. first, 2c and 3abc genes were amplified individually from p2 and 3abc postive clones and ligated together using pcr method, then this 2c3abc product was cloned into pgem - t easy vector and transformed e. coli dh5a competent cell. a postive recombinant plasmid which contained whole 2c3abc gene had been confirmed by pcr, enzyme digestion and sequencing. after that, the 2c3abc gene was sub - cloned into ppiczaa expression vector and transformed e. coli dh5 a competent cell and selected by zeocin ? antibiotic. the postive recombinant expression vector was linerized and electro - transformed pichia pastoris smd1168 competent cell. a recombinant pichia pastoris had been obtained by zeocin ? antibiotics selection and induced with 0. 5 % methanol for target protein expression. the expression product was analysised by sds - page and western blotting assay. the result sh owed that 2c3abc gene was expressed successfully in pichia pastoris and the product was a 95ku fusion protein which could be recognized by anti - fmdv serum. the amount of target protein was over 15 % of the total bacteria protein by gel thin layer scanning analysis. this research had supplied materials for establishing a fmd diagnosis method to differentiate infected animals from vaccinated animals
首先，用p2和3abc陽性克隆通過連接pcr方法獲得目的基因並將其克隆到pgem - teasy載體上，並轉化e colidh5a感受態細胞中，經pcr 、酶切以及測序證明得到了完整的2c3abc基因，並與國內外參考序列進行比較分析。然後，將目的基因亞克隆于ppiczaa表達載體並轉化大腸桿菌dh5a ，以zeocin ~ （ tm ）抗性篩選陽性克隆，大量提取重組表達質粒並用pme酶線性化后電轉化入畢赤酵母smd1168感受態細胞，通過zeocin ~ （ tm ）抗生素梯度濃度篩選，獲得重組酵母用0 . 5甲醇誘導表達，通過sds - page電泳、 westernblotting分析，結果表明， 2c3abc基因在畢赤酵母中成功表達，其表達產物為一95ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。 -
When the body suffered from malignancy, on the one hand, tumor restrained cell immunity function, antibody ' s production and the body ' s immunity effect through inducing restraining t cell, so it accelerated tumor ' s growth, on the other hand, tumor induced body fluid restrained gene, which prevented t cell and nk cell from killing tumor and restrained lymphocyte to mitotic multiplication reaction, indeed it accelerated tumor ' s growth
當機體一旦患上惡性腫瘤后，一方面腫瘤通過誘導抑制性t細胞，抑制細胞免疫功能及抗體的產生，抑制機體對腫瘤的免疫效應，從而使腫瘤逃逸機體的免疫監視，促進腫瘤的生長。另一方面，腫瘤通過誘導體液抑制因子阻止t細胞和nk細胞的等對腫瘤的殺傷作用，抑制淋巴細胞對有絲分裂原的增殖反應，甚至促進腫瘤的生長。
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