infected cell 中文意思是什麼

infected cell 解釋
受感染細胞
  • infected : 被傳染的
  • cell : n 1 小室，單室；隔間，艙；〈詩〉茅舍；（單個的）蜂窩，蜂房。2 〈詩〉墓穴，墓。3 （大修道院附屬的...
  1. The characteristics of tm - 22 expression presented in transgenic tobacco : 1 ). virus specificity in either homozygote or heterozygote ; 2 ) tm - 22 gene integrated in tobacco genomic dna with single copy and in inheritance and segregation to progenies on the mendel role ; 3 ). transgenic line with tm - 22 promoter ( ptm47 ) showed infected symptoms with cell death distinguished to one with 35s promoter ( ptm49 ) after inoculation with tomv - 2a

    其次，通過氨基酸序列和結構的比較，確定tm - 2 ~ 2基因的編碼蛋白與tomv病毒在抗病反應中相互識別的特異氨基酸及其功能；然後，應用重組dna技術，互換tm - 2 ~ 2基因和tm - 2基因的對應結構域，構建嵌合基因，獲得嵌合蛋白表達的轉化體，驗證tm - 2 ~ 2編碼蛋白中變異氨基酸的作用。
  2. A pair of primers were chemically synthesized based on the cdna sequence of hog cholera virus strain brescia and used to amplify the cdna fragments of envelope glycoprotein e2 gene of hclv which coding the major protective antigen by reverse transcription - polymerase chain reaction ( rt - pcr ) from total intact rna extracted from infected calf testicle cell culture by hclv

    以豬瘟兔化弱毒犢牛睪丸細胞毒（ hclv ）中提取的細胞總rna為模板，以rt - pcr技術，擴增出了完整e2基因的cdna片段。經電泳、酶切分析，證實了所擴增片段為e2基因特異性片段。
  3. In the adherent culture, the virus did not result in cell lysis after the insect cells were infected

    而在本論文中，我們嘗試將此一非溶裂昆蟲細胞/桿狀病毒表現系統轉移到旋轉瓶的懸浮培養。
  4. After the recombinant plasmid pcdna3. 1 / ts87 was identified by digestion of hindlll and bamh i, it transformed into cos7 by lipofectamine. expression product was identified by immunohistochemical method, sds - page and western - blot. the immunocytochemistry result has shown that specific brown - staining grains were found in the cytoplasm of cells transformed by recombinant plasmid versus not seen in cells transformed by pcdna3. 1 or normal cells ; the sds - page result has revealed that a band about 3 8kb was found in cell lysis transformed by recombinant plasmid versus not in cells transformed by pcdnas. l or normal cells ; the western - blot result has showed that only the band about 38kd was recognized by sera from rabbit infected by t. s artificially and sera from rabbit immunized with soluble antigen of t. s and with protein expressed by ts87 gene and by a monoclonal antibody of t. s

    通過細胞的免疫組化，細胞裂解物的sds - page電泳， westem - blot分析檢測目的基因的表達情況。免疫組化結果顯示：重組質粒轉染的細胞質中有棕褐色顆粒，而空載體轉染細胞及正常細胞無此現象；細胞裂解物sds - page電泳結果顯示：只有重組質粒轉染的細胞在約38kd處有明顯的蛋白帶，這與理論計算的ts87基因表達蛋白的分子量為38kd基本一致； western - blot分析結果顯示：約38kd的蛋白帶能夠分別被旋毛蟲感染兔血清，成蟲蟲體可溶性抗原免疫兔血清， ts87基因原核表達蛋白免疫兔血清（ ts87血清）以及一株具保護性的旋毛蟲單抗特異識別。
  5. The env protein deduced from env gene encodes the hydrophilic surface protein ( su ) and the hydrophobic transmembrane domain ( tm ) that determine the specific interaction between virus particles and cell surface receptors during retroviral entry. the su of retroviruses is a highly variable genetic element, containing receptor binding sites and major antigenic determinants. exjsrv - specific dna probes were derived. by using these dna probes in tissue hybridization. we successfully identified jsrv mrna expression and proviruses dna in sheep lung tissues infected with jsrv and control group has no postive signals, validating the use of exogenous virus - specific dna probes in the analysis of oncogenic proviral integration sites and identification of integrated exogenous proviral sequences

    用地高辛隨機引物法標記exjsrv特異的env片段，制備探針，原位雜交檢測spa肺組織中的rna及前病毒dna ，結果表明spa患羊肺組織內有jsrvenv基因mrna的表達，同時也檢測到了前病毒dna ，而相應的陰性對照卻無陽性信號，證實外源性病毒特異的dna探針在致瘤性前病毒的整合位點和整合的外源性前病毒的檢測中具有可信度。
  6. The eiav - pok8. 2 - his was transfected into the donkey leukocyte culture. following an incubation period, reverse transcriptase activity was detected in cell culture supernatants. cytopathic effect was observed by no. six passages post - infection in donkey leukocyte infected by the virus derived from eiav molecular clones eiav - pok8. 2 - his

    提取前病毒dna ， pcr擴增p1p4片段，亞克隆至pmd18 - t載體，測序證明前病毒dna含有六個組氨酸，從而在體外獲得了感染性克隆病毒株。
  7. Meq protein, highly expressed in the insect cell line sf9 by the baculovirus vector was immunized into balb / c mice and the immunized spleen cells were collected and fused with the tumor cell line sp2 / 0 via peg - 1000 in vitro. the hybridoma cells were cloned and screened for the ability of anti - meq mcab secretion by fa with the mdv ga infected chicken embryo fibroblast ( cef )

    利用通過桿狀病毒載體在昆蟲細胞系sfg上高度表達的meq蛋白產物免疫balb / c小鼠，然後收獲其免疫脾細胞並與腫瘤細胞系spz / 0通過peg于體外融合;獲得的雜交瘤細胞被克隆並通過與mdv感染的雞胚成纖維細胞（ cef ）做免疫熒光試驗（ fa ） ，進行其分泌抗meq單克隆抗體（ mcab ）能力的篩選。
  8. From dead chicken which infected infectious stunting syndrom of our province, one virue was isolated using spfeggs, chicken embryo fibroblast, mdck18, and vero cell. this virus was unable to agglutinate chicken erythrocytes. in order to definite the pathogeny of infectious stunting syndrom. physical and chemical specific property, types of the nucleic acid of the isolated virus, recurrent infection and other biological property determination and indirect elisa test proved it as a parvoviruses like strain of chicken

    為確定該病的病原，對所分離病毒進行了理化特性測定、病毒核酸型別測定、動物回歸試驗等生物學特性測定，證明該分離病毒與細小病毒科（ parvovirdae ）細小病毒屬（ parvovirus ）的雞細小病毒（ chickenparvovirus ）特性基本相符，核酸型為dna型。
  9. Biological activity was determined by egf dependent balb / c 3t3 cell line and with mtt colorimetric assay. extracts of the recombinant virus - infected and mock - infected cells, haemolymph of the recombinant virus infected and mock - infected silkworm larvae could all support the proliferation of balb / c 3t3 cell. this phenomena implied that there were some egf - like growth factors in the haemolymph of normal silkworm larvae, which could enhance the proliferation of the cell line

    用小鼠balb c3t3成纖維細胞和mtt法測定表達產物的促細胞增殖作用，發現重組病毒感染家蠶細胞72小時的胞內樣品與正常家蠶細胞裂解物，以及重組病毒感染4天的蠶血淋巴與正常蠶血淋巴均具有相似的促細胞增殖作用，甚至野生型病毒感染的細胞裂解物和蠶血淋巴也有一定的細胞促生長作用，提示家蠶系統本身可能含有能促進細胞生長、類似於egf的細胞因子。
  10. The protein product of meq gene was highly expressed in the nuclei of recombinant baculovirus infected sf9 cells when using an anti - meq monoclonal antibody ( mcab ) 23b46 to run the immunofluorescence assay ( fa ) ; the expression quantity and if staining patterns differed with different times post - infection ( pi ). the results of western blotting and immunoprecipitation test showed there were two specific bands around 60 kd. the results of the study demonstrated that the baculovirus / insect cell system is effective to be used to express nuclear protein of virus

    結果發現:本表達系統產生的meq蛋白可被重組痘病毒表達的meq制備的單抗23b46所識別;在感染細胞中， meq蛋白僅局限於細胞核內，而且隨著感染后（ pi ）時間的增加，具有從核質向核仁和核膜轉移的趨向; w已stemblot和免疫沉澱試驗均證實重組桿狀病毒感染細胞裂解物中出現有兩條大小約為60kd的特異帶。
  11. Thin sections of host leaf cells infected by bbwv - 2 isolate b935, which were gold - labeled by antibodies of bbwv - 2 coat protein ( cp ) and vp37, respectively, were prepared to elucidate the locations of vp37 in cell and possible function of vp37 and cp in cell to cell movement. observation in electron microscope showed that virus particles were presented not only in cytoplasma but also in chloroplast, while vp37 was existed only in cytoplasma and associated with tubular structure through the cell wall

    為研究vp37在寄主細胞中的作用機制及其在細胞中的分佈，通過膠體金間接標記6his - vp37兔抗血清，同時還標記了病毒的外殼蛋白單克隆抗體，對bbwv - 2分離物b935感染的病葉超薄切片的電子顯微鏡觀察發現：病毒粒子除了聚集在胞質中，還存在於寄主的葉綠體內； vp37蛋白能在細胞壁上形成管狀結構，在胞質中亦有分佈。
  12. The positive staining were found in the mdv plaques and localized on the cytoplasme of the infected cell

    表明大腸桿菌中表達的pp24融合蛋白保留有特異的抗原性。
  13. Of particular interest are " memory cytotoxic t cells ", because people infected with certain viruses, especially cytomegalovirus ( which most of us have, in fact ), accumulate a huge number of this type of immune cell that is specifically reactive against proteins encoded by that virus

    特別有趣的是"記憶細胞毒t細胞" ，因為被某些病毒感染的人，特別是被細胞巨化病毒感染的人（實際上我們大部分人都被它感染） ，大量積累特異地起反應來抗擊該病毒編碼的蛋白質的此類免疫細胞。
  14. The viral nucleic acid contains information necessary for programming the infected host cell to synthesize virus - specific macromolecules required for the production of viral progeny

    病毒的核酸含有使感染細胞合成子代病毒所需的病毒特異性大分子的所有必需的信息
  15. The close genetic relationship of goose parvoviruse and aav allows the examination of the molecular biological properties of the nonstructural proteins of gpv. after the gpv infected the cell the viral life cycle was regulated by the nonstructural proteins encoded by the virus. according to the published of gpv b strain genome nucleotide sequences in genbank and a pair of specific primers were disigned with oligo4. 1

    本研究根據genbank發表的gpvb株全基因序列，藉助oligo4 . 1軟體設計一對引物，採用pcr技術擴增gpvh1株非結構蛋白ns2基因，並與pmd18 - t載體連接后測序，結果表明：鵝細小病毒h1株ns2基因核苷酸全長1356bp ，編碼451個氨基酸殘基，與gpvb株的ns2基因相比，核苷酸數目相同，有17個堿基、 6個氨基酸的差異；同源性分析表明：二者核苷酸序列同源性為98 . 75 ，推導氨基酸序列同源性為98 . 67 。
  16. Comparison of cytopathic effect of sf21 cell and embryo cell of silkworm infected by nosema bombycis

    家蠶微孢子蟲侵染家蠶胚胎細胞與草地貪夜蛾細胞的病變比較
  17. Interferon ( ifn ), a family of cytokines, have activities on interfering with the replication of various viruses, decreasing cell proliferation and modifying immunological processes. the research about ifn indicates, it is a kind of ideal therapy that ifn act on healthy or unhealthy animals to prevent or cure many kinds of diseases infected by virus

    干擾素（ ifn ）是一類重要的細胞因子，具種屬特異性，在同種細胞上具有廣譜的抗病毒、抗細胞增殖、免疫調節等多種生物活性，研究表明，利用干擾素防治家畜的病毒性疾病是一種理想的治療方式。
  18. A - l was infected on monolayer sffi ther the third plaque purification to reproduce in quantity and named it a - 2. after a - 2 was infectal on sffi monolayer cell 4d harvested the cpe cell. the analysis results with sds - page and dot - elisa showed that prv vp7 gene was - - o - - abstract expressed in baculovirus

    接種a - 2代病毒于sf9單層細胞上， 4d后收獲病變細胞，通過sds -聚丙烯酰胺凝膠電泳（ sds - page ）和免疫酶斑點技術（ dot - elisa ）分析，表明prv - vp7基因在桿狀病毒表達系統中得到了表達。
  19. Panning and enrichment ofmg7 recombinant phage antibody the transformed cells were infected with m13k07 helper phage to produce a phage form of mgy recombinant phage antibody library ; after three rounds panning of the library with the mgrbinding antigen highly expressed gastric cancer cell line kato iii, the mg7 scfv displayed phage dones were selected from the enriched recombinant phages by elisa

    3 mg _ 7重組噬菌體抗體庫的淘篩用m13ko7輔助噬菌體感染轉化菌，以挽救出噬菌體形式的抗體庫；用高表達mg _ 7抗原的胃癌細胞系kato對抗體庫進行三輪淘篩；用elisa篩檢呈現有mg _ 7scfv的噬菌體單克隆。
  20. There are some vesicles near the cell walls of senescant cells, they do not derive from the plasma membranes sink of normal infected cells, but the disintegration of membrane system of senescant cells

    在衰老侵染細胞中，細胞壁附近有小泡，它們可能是由細胞中的膜系統降解而成。
分享友人
分享到 Line

分享到臉書