inner colonies 中文意思是什麼
inner colonies
解釋
近地殖民地-
After 4 days of growth, the intact inner cell mass ( icm ) were separated with 0. 05 % trypsin - edta and replated on feeder layer in dmem containing 15 % serum, 0. 1mmol / l nonessential amino acids, 0. 1mol / l 2 - mercaptoethanol, 2mmol / l glutamine, 100 units / ml of streptomycin and 100 units / ml of penicillin. after 4 - 6 days 5 es cell colonies were selected and expanded in which alkaline alkaline phosphatase was detected
（ 2 ）小鼠囊胚或內細胞的培養和es細胞的分離培養了156個不同品系的小鼠囊胚，經過3 ? 4天培養后，將增殖出來內細胞團用機械法結合胰酶- edta處理，離散后培養于mef飼養層上， 4 6天後有5例出現了es細胞樣集落。
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