integrated plasmid 中文意思是什麼

integrated plasmid 解釋
整合質粒
  • integrated : adj. 完整的，完全的。 an integrated iron and steel works 鋼鐵聯合企業。 an integrated oil company 大型石油（聯合）公司。
  • plasmid : 質粒；原核質體
  1. Methods : an artificial gene for fl extracellular domain cdna was synthesized by using favored genetic codons of pichia pastroris. by inserting human fl extracellular domain cdna coding 156 amino acid resi dues into pichia pastoris expression vector ppic9k containing aox1 promoter and the sequences of alpha secreting signal peptides, a recombinant expression plasmid ppic9k - fl was constructed, and integrated into the alcohol oxidase region of the host genome

    為了提高外源基因的表達量，我們根據畢赤氏酵母偏愛密碼子人工合成了編碼fl胞外區156個氨基酸的cdna序列，目的序列被定向克隆到酵母分泌型表達載體ppic9k質粒上，構建ppic9k - fl表達質粒。
  2. Construction of male sterility expression vector by integration of artificial sense and antisense cdnas of hsp70 into puc18 and puc19 respectively, we can obtain psc and pac. tapertal specific expression promoter ta29 and terminator nos are connected directionally with sense and antisense cdnas of hsp70 extrected and purified from psc and pac., then integrated into puc18 and puc19, by which we can build sense and antisense cdna nos ( respectively named plasmid 650 and plasmid 651 ) of ta29 - hsp70. for the sake of better screening and examination of transformed gene, we cut plasmid 650, plasmid 651 and 3301 ( containing gusgene bar screening marker gene ) with hindiii and ecor i enzymes, then connect purified fragments of 650and 651 with plasmid 3301 to construct the vector 3301 + 650 and 3301 + 651. corroboration of whether sense and antisense cdna - nos is integrated into plasmid3301 can be made by plate screening and enzye - cutting analysis

    分別將從psc 、 pac回收純化的hsp70正、反義cdna與絨氈層特異表達啟動子ta29及nos終止子定向連接，然後插入到puc18 、 puc19中，構建成花藥特異表達的ta29 - hsp70sensecdna - nos和ta29 - hsp70antisensecdna - nos ，分別稱作650和651質粒。為了更好地對轉化子進行篩選和檢測，用hind和ecor分別對650 、 651及3301質粒（含gus報告基因和bar篩選標記基因）進行酶切，將從650和651回收純化的目的片段與3301質粒進行連接，再對重組子進行平板篩選和酶切分析確定ta29 - hsp70sensecdna - nos和ta29 - hsp70antisensecdna - nos插入到3301質粒中，構建成3301 + 650和3301 + 651表達載體。
  3. Dnd phenotype could be detected when the entire dnd cluster was integrated in the chromosome or carried by the low copy - number plasmid in zx1. however, when dnd cluster was carried by a plasmid with a copy - number around or higher than c. 10 copies in zx1, dnd phenotype could not be observed

    當完整的dnd基因簇整合在zx1染色體上或由1 2個拷貝的自主復制的質粒攜帶進入zx1 ，都能在zx1中檢測到dnd表型，但是由10個或超過10個拷貝的質粒攜帶進入zx1 ，就檢測不到dnd表型。
  4. Pcr analysis of resistant seedlings with nptii gene primers showed that 6 out of 12 seedlings detected had the 700bp fragment specific to the plasmid pig121, indicating that t - dna had been integrated into the genome of sweet cherry

    L ，負壓的適宜處理是lmmxio次。 6 、通過p r擴增，初步證明證明外源基回己轉入甜櫻桃。
  5. To abolish transcription efficiency affected by copy number or integration site in chromosome, all the vectors were integrated into frt site by co - transfecting with an flp recombinase producing plasmid

    應用w七stemblot對其中的20個克隆進行檢測，選出一個caspase一3表達量最低的細胞克隆a6 。
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