intron 中文意思是什麼

intron 解釋
插入序列
  1. The coding region of the cdna of the phytoene desaturase gene was cloned by pcr and inserted into vector pbi121 containing intron - kanamycin

    再生苗經過戍r和pcrs 。 : : hernbl 。 t分子檢測證明獲得了煙草轉基因植株。
  2. The length of this phytase gene is1506bp interrupted once by an intron of 102bp in the 5 " part of the gene, this intron contains donor sequence - gtatgc, lariat sequence - gctgac and acceptor sequence - cag which are typically conserved sequence of the intron of fungal phytase gene. this gene encodes a peptide of 467amino acid residues with molecular weight of 51. 37kda, containing 13 potential n - glycosylation sites and a signal peptide sequence made up of 19 amino acid residues at n teminal of the peptide

    核苷酸序列分析表明， pcr擴增產物中包含有完整的phya基因，該基因全長1506bp ，其中包含一段長102bp的內含子，該內含子具有真菌植酸酶基因內含子的特徵保守序列： donor序列? gtatgc ， lariat序列? gctgac及acceptor序列? cag 。該基因編碼467個氨基酸，理論分子量為51 . 37kda ，其上有13個潛在的n -糖基化位點， n端19個氨基酸為信號肽序列，植酸酶活性位點序列（ crvtfaqvlsrhgaryptdskgk ）位於氨基酸序列的+ 71 + 93 。
  3. To study on structure and inheritance of rh d gene interaction between gene expression of rh d and rh c / e and influences on rh d gene expression of inserts and rh box - methods : 20 pairs of oligonucleotide specific primers for exon, intron 2 4, insert and rh box of rh d, rhc / e gene were designed and composed the polymerase chain reaction - sequence specific oligonucleotide primer ( pcr - ssp ) was used to amplify the rh c / e gene, rh d gene, exon, intron, insert and rh box in 106 samples of unrelated individuals and 7 han nationality ancestries and 5 wei nationality ancestries whose proband were rh d - negative

    目的：觀察中國漢族非血緣關系隨機個體、漢族及維吾爾族家系rh血型的c e基因、 d基因外顯子、內含子、插入片段以及rhbox ，研究rhd基因結構及遺傳規律， d基因表達與c e基因的關聯，以及插入片段和rhbox對d基因表達的影響。
  4. Progress in the research of mutation of genetic intron and retinitis pigmentosa

    基因內含子突變與視網膜色素變性的研究進展
  5. Based on cdna sequences of ( 3 - carotene ketolase ( cr / ff ) and p - carotene hydroxylase ( c / - fz ), a 1. 6 kb crtw genomic sequence with six introns, and a 3kb crtz genomic sequence with five introns were clone, respectively. all the exon - intron junctions conform closely to gu - ag consensus splicing rule

    本論文工作成功地克隆兩個關鍵酶基因的基因組序列，發現crtw和crtz分別包括6個和5個內含子序列，所有這11個內含子的剪切位點都符合gu - ag規律。
  6. Intron polymorphisms of pit - 1 gene in xiang pig

    1基因內含子多態性的研究
  7. Genetic variation in 5 ' - upstream region and the second intron of h - fabp gene in nine pig breeds

    上游區和第二內含子的遺傳變異
  8. Intron a noncoding dna sequence that occurs between coding sequences ( exons ) in many eukaryote genes

    內含子：是大多數真核生物基因中位於編碼序列（外顯子）之間的不編碼的dna序列。
  9. And the intron had a lot of gt repeated sequence. the dna and protein sequence of this gene was analyzed using the bioinformatics tools. two functional domains were found in the protein

    運用生物信息學手段對3一磷酸甘油脫氫酶基因核酸以及蛋白質序列做出了分析，發現這個基因編碼兩種功能的結構域，磷酸化酶結構域和3一磷酸甘油脫氫酶結構域。
  10. Compared with bombyx. mori, the exon regions of alpha - amylase gene of bombyx mandarina have 13 transitions, 4 transversions and a 12bp insertions, while the intron regions have 362 transitions, 343 transversions, a 94bp insertions and a 56bp deletions. the whole alpha - amylase gene ( including the 5 ' - and 3 ' - utrs ) of bombyx mandarina has 380 transitions, 350 transversions, 113bp insertions and 57bp deletions

    野桑蠶相對於家蠶來說，在-澱粉酶基因外顯子區域發生了13次轉換、 4次顛換、 12bp插入，在內含子區域發生了362次轉換、 343次顛換、 94bp插入和56bp缺失，全基因（包括utr ）共發生mmrttoopmi mgzx ffatt了380次轉換、 350顛換、 113hp插人和57hp缺失。
  11. As an insect expressing system, silkworm has long been thought highly of. however, because the relation between insects and mammals is in evolution is so distant, we do not have the exact idea if the introns of the mammalian gene can be spliced correctly in insect cells and what influence the intron imposed upon the gene expressed in the insect cells

    家蠶作為一種昆蟲表達體系受到人們的重視，但是由於昆蟲與高等哺乳動物在進化上相距較遠，高等哺乳動物基因的內含子能否在昆蟲細胞中正確剪切，內含子對此基因在昆蟲細胞中表達影響如何，目前有許多的爭論。
  12. 4, an intron sequence was also inserted upstream of gfpmut3 and its six reading frame could all be stopped, which could guarantee gfp translation in right reading frame

    將藍色熒光蛋白基因bfp克隆到pet - 11c上，轉化bl21 （ de3 ）后實現了bfp在大腸桿菌中的誘導表達。
  13. A pair of primers was designed based on the conserved regions of other higher plants " epsp synthase through homology alignments. two dna fragments were first cloned from o. violaceus by performing prc which used o. violaceus genome as the template. one has 798 nucleotides and the other 1157 nucleotides, but they can encode the same amino acid sequence and have same extrons according to the gt - ag rule of characteristic sequence of enkaryoutic intron

    根據同源比較其它高等植物中epsp合成酶基因，找出該基因的保守序列並設計一對寡核苷酸引物，以諸葛菜的總dna為模板進行pcr反應，克隆出了兩個epsps基因的片段，其中一條長為797bp ，另一條1157bp ，它們在genbank的登錄號為： af440390 、 af440391 。
  14. The sequences of intron, however, had only 74 % of homology with those of a. thaliana ban. in addition, amino acid sequence of oilseed rape ban had 78 % of homology and 87 % of similarity with those of a. thaliana and had various homologies ( 55 - 64 % ) with many nadph - dependent reductases of other plants

    將甘藍型油菜ban氨基酸序列進行同源比較，發現甘藍型油菜ban片段的181個氨基酸與擬南芥有78的氨基酸序列相同，相似性達87 ；與80多種植物的nadph還原酶類也有不同程度的同源性（ 55 - 64 ） 。
  15. Moreover, asp immediately preceding the active site ser is necessary for proper folding of the catalytic site. the human clsp cdna corresponded to unigene cluster hs. 415792 located on human chromosome 12pl3. 31. nucleotide sequence alignment of clsp and this sequence enabled us to demonstrate the genomic structure of the clsp gene with appropriate exon - intron boundaries, which contained 6 exons and 5 introns

    Clsp在造血細胞系的表達較為特殊，不表達于淋巴瘤細胞系，只表達于單核髓系白血病細胞系，如慢性髓系白血病k一562 、髓系單核白血病thp一1 、前髓系白血病hl一60和b4 ，以及急性髓系白血病kg一1細胞系，其中thp一1細胞系經lps刺激后clsp表達水平亦呈上升趨勢。
  16. The 2nd intron of the s7 ribosomal protein ( rps7 ) gene is first used to examine the relationships among sisorid fishes

    將s7核糖體蛋白基因內含子2首次用作遺傳標記進行中國?科魚類的系統發育分析。
  17. The length of the 2nd intron sequences obtained by pcr amplification ranges from 153 to 168 bp in the 11 sisorid species ( 17 samples ) investigated, and is 154 bp in liobagrus kingi and liobagrus marginatoides

    通過pcr技術，擴增了長度為154bp的金氏（魚央）和擬緣（魚央）以及長度為153 168bp的11種?科魚類（ 17個樣品）的rps7基因內含子2序列。
  18. This vector, including sbe2b gene, was 6. 06kb long, containing endosperm specific promoter and a intron that enhanced the transcript efficiency. both sense and antisense cdna were transformed to upregulate and downregulate amylose content and relative branching characters

    正向連接的表達載體用於增加胚乳中支鏈澱粉的含量，反向連接用於減少胚乳中支鏈澱粉的含量，增加直鏈澱粉的含量。
  19. This expression vector plbcas - hsa - lgl has the following advantages : i ) the 1. 7kb promoter is able to drive cell - specific and hormone - dependent expression ; ii ) the inclusion of intron - 1 can increase expression level of fusion genes ; iii ) the 5 ' utr of bovine p - casein mrna may have a positive role in both transcriptional and post - transcriptional regulation ; iv ) the gfp gene make the selection of positive clone among embryos possible ; v ) the gfp gene can be easily excised via cre - mediated recombination between the two loxp sites after the expression vector has been integrated into chromosome ; vi ) the two incompatible lox sites, loxp and lox2272, would facilitate cre - recombinase mediated cassette exchange ( rmce ), which in theory will leading to develop a technology of site - specific gene expression in animal mammary glands

    該載體的特點是：具有可以調控外源基因在乳腺中特異表達的牛-酪蛋白基因5 `端側翼區和包括第一外顯子及內含子在內的5 `端調控區；將人血清白蛋白cdna準確地置於牛-酪蛋白基因第二外顯子中的翻譯起始密碼子atg之後，而且沒有增加額外的序列和使人血清白蛋白cdna移碼；引入標記基因gfp ，便於在胚胎期鑒定陽性胚胎，減少受體；引入cre lox重組系統： （ ? ）標記基因gfp的兩端的兩個loxp位點可以在表達載體整合到基因組后，刪除標記基因； （ ? ）餘下的一個loxp位點可以和前面的lox2272位點組成cre重組酶介導的盒式交換系統。
  20. Then, 5. 5kb thrombiotin gene was amplified with the same technique from the genome of a baby ' s blood, which included the begining part of intronl to the teminator. in addition, 6. 0kb and 1. 8kb homlogous arms were also amplified from a cow with high yield. the 6. 0kb homologous arm contains the promotor, extron 1, extron2, extron3 and intron 1, intron2 and part of the intron3 fragment, while the 1. 8kb homologous right arms contains exon13, exon14 and part of intron 13, the whole intron14 and part intron 14 of asl - casein gene of bovine

    通過長片段pcr從高產奶牛的基因組中獲得了打靶所需的長、短同源臂序列，長度分別為6 . 0kb和1 . 8kb ，位於s1 -酪蛋白基因的5上游區到第三內含子和十二到十四內含子；從綿羊全血基因組克隆得到了綿羊的-酪蛋白基因啟動子區到第二內含子區4 . 1kb的5調控序列；利用同對引物克隆得到了水牛的同基因序列；從廣西當地一嬰兒臍血基因組中通過獲得了人血小板生成素基因，位於第1內含子到終止子後部分的序列，長達5 . 5kb 。
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