isoelectric protein 中文意思是什麼
isoelectric protein
解釋
等電蛋白質- isoelectric : adj. 【物理學】等電位的，零電位差的。 isoelectric point 【物理學】等電離點。
- protein : n. 【化學】朊，蛋白（質）。
-
Fourthly, according to the activity, collect, dialyse, freeze, dry respectively the sod protein through deae - sepharose column chromatog - raphy ; process the sod protein through sephacryl s - 200 column chromatography with the preceding method. at last, process the pure sod protein with same functional enzyme electrophoresis and active dye, isoelectric focusing electrophoresis and sds - page
將粗酶液過deae -瓊脂糖柱層析，得三個活力峰，分別收集、透析、乾燥濃縮后；再上sephacryls - 200凝膠柱層析，按與deae -瓊脂糖柱層析后同樣的方法收集處理。 -
Varietal identification of oat avena spp. by ultrathin - layer isoelectric focusing of seed protein
超薄層等電聚焦電泳技術檢驗燕麥種子的真實性 -
Differences of seed protein from rice oryza spp. varieties identified by ultrathin layer isoelectric focusing
法對番茄雜交種子純度和真實性鑒定的研究 -
After isoelectric focusing, it gave a band at ph 8. 25. agglutination test suggested that the protein was a lectin
由於殼聚糖結構的特殊性，因此，它可被用來作為親和層析的載體 -
After quantification, the protein samples were separated by isoelectric focusing electrophoresis and then by sds - page
定量后，分別進行一維等電聚焦分離和二維聚丙烯酰胺凝膠電泳分離。 -
The full orp encodes a 487 - amino acid protein with a calculated molecule weight of 53. 484 kda and an isoelectric point of 6. 75. at the primary sequence level, it shared high homology with clr, so was named clr - like serine protease, clsp. the clsp sequence has been submitted to genbank / embl ( genbank accession number af178985 )
該蛋白在核酸和氨基酸水平上與人補體組分cl :表現高度同源，故將其命名為補體cl :樣絲氨酸蛋白酶（旦lr一like旦erineprotease ， clsp ） ，該基因已經在ge心ank登錄（登錄號為af17s985 ） 。 -
The results from sds - page presented that there were three female specific protein subunits with molecular weights of 123 kd, 120 kd and 91 kd, respectively. we can conclude the higher molecular compose of two subunits ; the results from two dimension electrophoresis showed the isoelectric points of two female - specific spots with molecular weight of about 120kd were 5. 5 and 5. 7. immunodiffusion reactions demonstrated that vg existed both in female fat body and hemolymph, which as vn was deposited in the ovary, while not in the male
Page電泳結果表明：麗蠅蛹集金小蜂明顯存在2條雌特異性帶-卵黃蛋白，分子量分別為181kd和136kd ； sds - page電泳分析：存在3條雌特異性帶，其分子量為123kd 、 120kd和91kd ，由此，可推定卵黃原蛋白（ vitellogenin ， vg ）和卵黃磷蛋白（ vitellin ， vn ）由2個蛋白組成，其中分子量較大的蛋白由2個亞基組成；雙向電泳結果顯示，在120kd附近有兩個特異性點，其等電點為5 . 5和5 . 7 ；雙擴散表明，麗蠅蛹集金小蜂卵黃磷蛋白的抗血清與雌隱成蟲蟲體、脂肪體、血淋巴和卵巢勻漿液均有免疫沉澱反應，而與雄蜂血淋巴無免疫反應，說明了vg與vn具有免疫同源性，是雌特異性蛋白，且由脂肪體合成。 -
At last, we got the pure protein with the determination of electrophoresis. the molecular weight of the enzyme is estimated at 30903 through gel filtration and sds - page electrophoresis. the isoelectric point is 8. 6 ?. 3
經sds凝膠電泳和凝膠過濾層析兩種方法測定該酶的分子量分別為33113dalton和28840dalton ；其等電點pi = 8 . 6 0 . 3 。 -
Firstly, the eo genes of csfv - jl and csfv - ln9912 strains were amplified by rt - pcr and nested pcr and then sequenced after cloned into t easy vector. comparing the eo sequences with other strains eo by biosoftwares, dnastar5. 0 and bioedit, the phylogenetic analyse revealed that all of the strains we have sequenced could be divided into groupl and groupii. two amino acid streches of 15 csfv strains eo, which form the rnase active site, and histidine residues essential for rnase catalysis in both ones were highly conserved. the eo protein propertys of antigen epitope, hydrophobicity, isoelectric point were also predicted by bioinformatic method
首先，採用rt - pcr和nest - pcr技術擴增了csfv - jl株和csfv - ln9912株eo基因，插入teasy載體后測序，應用dnastar5 . 0和bioedit軟體將其與本實驗室已測其它毒株和genebank中登錄的csfv代表毒株eo基因序列進行比較，繪制了遺傳進化樹並預測了eo蛋白的抗原表位、疏水性、等電點等特性。
分享友人