isolated cells 中文意思是什麼
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We hope that our study will provide us with more comprehensive knowledge of the mechanisms of immune regulation and the roles that dc and complement play in innate and acquired immunity, as well as to lay a foundation for further exploration of the roles dc play in antigen - specific immune responses and immune tolerance from a new perspective. part i expression of complement receptors and complement - associated molecules on dendritic cells derived from distinct - origin at different stages of development two subsets of dendritic cells were generated from precursor cells isolated by means of magnetic cell separation system
曰補體受體及其相關分子在modc上的表達不同分化階段的單核細胞衍生性dc （ monocytes一deriveddc ， modc ）的誘導:將新鮮分離的單核細胞mo ，在含有gm一csf和工l一4培養體系中誘導5一7d ，即分化為未成熟modc ;對培養至sd的未成熟modc ，用tnfa刺激zd ，即分化為成熟modc ;此時再用lps刺激24h ，即為活化的modc 。 -
Biotransformation in organic solvent is an attractived field in nowadays. compared with isolated enzyme whole cell is not used broadly as biocatalyst. in this research the cells of baker ' s yeast is adopted to mediated a model reaction in organic solvent, in which geraniol is converted to citronellol reductively
有機相生物轉化是當前生物技術中一個具有理論研究意義和應用價值的領域，目前該領域的研究大多集中在利用分離的酶進行生物催化，利用微生物完整細胞進行的研究比較少。 -
3 ) a heterotrophic nitrifier, named strain hn, was isolated from the greenhouse soil. the cells of isolates were gram positive, rod or coccus
一3 ）從土壤中分離到一株異養型硝化細菌，命名為菌株hn ，分離菌株為革蘭氏染色陽性，球狀或桿狀。 -
Objective to investigate the effects of fluid shear stress on il - 8 gene in human umbilical vein endothelial cells ( huvecs ) and the roles of time course of low shear stress and intensity of fluid shear stress using lightcycler ? system ; to investigate the gene expression profiles in huvecs exposed on low shear stress ( 4. 20 dyne / cm2, 2 h ) and incubated by 17 p - estradiol ( 10 - 7 m ) + low shear stress ( 4. 20 dyne / cm2, 2 h ) using the cdna microarray approach. methods endothelial cells were isolated from human umbilical cord veins by collagenase treatment as described by jaffe and modified
目的探討人臍靜脈內皮細胞（ humanumbilicalveinendothelialcells ， huvecs ）在流體切應力作用下il - 8基因的誘導表達以及切應力的作用時間和作用強度對il - 8基因表達影響的變化規律；利用表達譜基因晶元技術研究內皮細胞在低切應力（ 4 . 20dyne cm ~ 2 ， 2h ）作用下其切應力相關基因的表達情況以及生理濃度（ 10 ~ （ - 7 ）的17 -雌二醇孵育內皮細胞48h ，再經同樣條件的切應力處理后對內皮細胞切應力相關基因表達的可能影響。 -
Embryonic stem ( es ) cell lines isolated from primitive embryonic cells are highly undifferentiated and totipotent cells. in vitro, es cells can spontaneously differentiate into embryoid bodies ( ebs ) containing derivatives of three germ layers
胚胎幹細胞（ embryonicstemcell ， escell ）系是從早期胚胎細胞分離培養而建立的高度未分化的具有全能性的細胞系。 -
Whole mount in situ hybridization showed that amphihmgb transcripts were present ubiquitously in the early embryos from zygote to neurula, but they decreased gradually in mesoderm and endoderm and neural tube with development, and became restricted to epidermal cells in the hatched larva. it appear s that hmgb plays a role in the mitosis and differentiation of embryonic cells. another amphioxus cdna, amphiubf80, encoding ubiquitin / ribosomal fused protein s27a, was isolated from the gut cdna library of b. belcheri tsingtauense
S004cdna序列編碼一個156個氨基酸殘基組成的蛋白質，與人的泛素蛋白ubf80具有較高的氨基酸序列同源性（ 145 / 156同源） ，且該蛋白具有泛素蛋白ubf80所共有的兩個結構域，即由72個氨基酸組成的泛素介導的蛋白降解結構域和由48個氨基酸組成的核糖體蛋白s27a結構域。 -
In the first trial, combination of enzymatic digestion was used to prepare suspensions of spermatogenic cells from adult mouse testis, and then a modified discontinuous percoll gradient centrifugation method ( 15 %, 22 %, 30 %, 40 %, 50 %, 60 % ) was introduced to isolate spermatids from the cellular suspensions. the content of spermatids in each isolated fraction by percoll method was determined by morphology ( wright - giemsa stain ) and flow cytometry analysis, and the viability of spermatogenic cells was assessed by using eosin y exclusion test
在第一部分試驗中，首先利用連續3次組合酶消化成年小鼠睪丸制備睪丸細胞懸液，然後經6層非連續percoll梯度離心法（ 15 、 22 、 30 、 40 、 50和60 ）分離，通過形態學和流式細胞術鑒定南京醫科大學碩士學位論文各個percoll組分中精子細胞的含量，並以伊紅y排斥試驗測定細胞的存活率。 -
In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis
擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。 -
After fertilization granulosa cells and oocytes were isolated and continued to be cultured for 48 hours, and made into suspension ; the levels of fsh - r on the the granulose cells shall be immediately measured. results : 1
受精後分離顆粒細胞中文摘要和卵母細胞，將所剝離下的全部顆粒細胞繼續培養48 』 j 』時，製成混懸液，並及時進行顆粒細胞膜上fsh丑含量的測定。 -
The results of biological tests have demonstrated that allantoic fluid of the first passage virus did n ' t produce macroscopic pathogenic role to chicken embryos and after passaged for four times, gross lesions were observed in chicken embryo. the virus showed typical coronavirus under electron - microscope and it could n ' t form plaque in cef cells and could hemagglutinates chicken red blood cells after treatment with 1 % trypsin. to surprise, the virus replicated in cef cells also showed hemagglutination activity to chicken red blood cells. in addition, the spf chickens which inoculated with the virus isolated from the chicken damaged tissue showed clinical sign and grow lesion, but it ' s gross lesion did n ' t resemble to those of field outbreaks
生物學特性：雞胚尿囊液經離心、磷鎢酸負染后，電鏡觀察該病毒為典型的冠狀病毒；該毒株的第一代尿囊液對雞胚無肉眼可見的致病作用，當繼代到第5代后，胚體嚴重病變；病毒在雞胚中隨著接種時間的延長，其效價增高， 96h可達到48h的2倍；該毒株可在cef上生長，但不能形成明顯的蝕斑；經1胰酶處理后可凝集雞紅細胞；雞胚的第四代尿囊液病毒回歸動物體，病死雞腎臟呈典型的花斑腎，腺胃則未見肉眼可見的病變。 -
A study on histamine release from mast cells induced by sinomenine and anti - histamine effect of sinomenine in isolated guinea pig ileumuijie
青藤堿組胺釋放作用與抗組胺收縮腸管作用研究 -
The reverse transcriptase isolated from human leukemic cells resembles that from rna tumour viruses in several respects.
從人白血病細胞分離得到的遞向轉錄酶在如下幾方面類似於RNA腫瘤病毒逆向轉錄酶。 -
According to the polarity, the residue was isolated with petroleum ether, chloroform, ethyl acetate, and n - buoh, respectively. the n - buoh fraction, confirmed as neuroactive component, was subjected to sephadex lh - 20 column chromatography to provide an extract fraction, as a buff powder, which could induce neurite outgrowth in rat pheochromocytoma pc12 cells in a dose - dependent manner up to 50 mg / l
將菟絲子乾粉用75乙醇浸泡后，減壓蒸干后得到褐色漿狀物，經石油醚、氯仿、乙酸乙酯、正丁醇萃取，經柱層析后，再用葡聚糖凝膠對有效成份進一步純化，獲得了菟絲子中能誘導pc12細胞分化的活性組分。 -
The isolated hepatocytes, isolated perfused organs, tissue slices, liver cell lines, subcellular fractions and the genetically engineered cells are the most commonly used in vitro liver models nowadays, especially the isolated hepatocytes
目前，常用的肝臟體外模型包括原代肝細模型、離體肝臟模型、肝臟切片模型、肝細胞系模型、亞細模型及基因工程細模型等，其中原代肝細型最?常用。 -
Representative cdna library from isolated rice sperm cells
水稻穗瘟防衛反應相關基因的分離和鑒定 -
The steady dead generation and time that was caused by the isolated virus was certain by chicken embryo which was inoculated on seven or nine days. the histopathological changs of the infectious stunting syndrom were studied by the way of ordinary paraffin section and he dying. the experimental result were as follows : the test proved that the changes of the chicken embryo were different in different stage. the chicken embryo dead in a week after it inoculated. the body was dropsy and hemorrhage. dead before it hatched out, the embyo body were dropsy, pale and slime. the liver was yellow and swolled, gallbladder ( vesica fellea ) was filled with bile. bursa and glandula thymus analosis. the kindey dropsy. bowel lamina were humble, dilatation. gas and yellow foam were filled the bowel. histopathological changes were that, in early stage, obvious changes of liver and kindey were dropsy, hemorrhage and necrosis. two types eosinophilic intranuclear inclusion bodies including large round and little granular were present in cells of the above organs. the obvious changes of bursa were dropsy, adverse folliiculated growth and little lymphocytes proliferating, 19 - 21 days chicken embryo, one or two big empty vacuoles were prensent in cells of liver and kindey. the number of the folliculi was growing, the vacuoles between cells were larger
膽囊充盈、其內充滿稀薄的膽汁；法氏囊、胸腺萎縮，腸道擴張、腸壁菲薄、內充滿氣體及黃色泡沫狀物；腎臟腫大。病理組織學變化方面，早期肝臟、腎臟、腸主要以出血、水腫和壞死為主，且肝細胞核及腎小管的上皮細胞核內均發現有核內包涵體，包涵體呈嗜酸性，為大型圓形包涵體或不規則的顆粒狀；法氏囊則以水腫、濾泡發育不良、小型淋巴細胞數量增多為主。 19 21日齡雞胚肝細胞、腎小管上皮細胞的胞漿內出現1 2各大的空泡，法氏囊濾泡數目增多細胞間有較大空隙。 -
To study the suitable method for cattle oviduct simple epithelium cells culture, the epithelium cells were isolated by cutting and 0. 25 % trypsinization, the exponential phase of growth cells vigor and growth velocity was determined by mtt method, the viable count was detected by the rejection experiment of trypanblau
摘要為探討適用於黃牛輸卵管單層上皮細胞的培養方法，採用機械剪取及0 . 25 %胰酶消化的方法分離獲得上皮細胞，取對數生長期細胞進行mtt比色實驗檢測細胞活力和生長速度；臺盼藍排斥試驗檢測活細胞數。 -
A water soluble crude polysaccharide in the cells has been isolated from the zymogen thread of hericium erinaceus pers. after the crude polysaccharide has been deamylumized by the combined methods of enzyme and sevage, the four graded component - hpa, hpb, hpc, hpd - would be abstracted by the method of ethanol grading
從猴頭菌（ hericiumerinaceuspers ）發酵菌絲體中提取出胞內水溶性粗多糖，經酶法和sevage法聯合脫蛋白后，用乙醇分級法分離出hpa 、 hpb 、 hpc 、 hpd四種級分。 -
Most observations on the glycocalyx have been made not on cells in contact but on isolated cells, usually in culture.
對糖萼的研究，大多數不是在接觸細胞，而是在分離的，通常是培養的細胞上進行。 -
The presence of isolated single cells is very suggestive of infiltrative carcinoma, but i have seen aspirates from pure dcis that contain these isolated cells
此例中細胞單一、分散更支持是侵潤性的癌，但是我曾經在一例乳腺導管原位癌的穿刺塗片中也看到這樣孤立單一的細胞。
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