isolated layer 中文意思是什麼

isolated layer 解釋
隔離層
  • isolated : 被隔離的
  • layer : n 1 放置者，鋪設者，計劃者。2 【賽馬】（一般）賭客。3 產卵的雞。4 【軍事】瞄準手。5 層；階層；地...
  1. In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis

    擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。
  2. Numerical simulations of mountain gravity waves generated by multi - layer flow over an isolated mountain

    三維多層流動過孤立山脈產生的山脈重力波的數值試驗
  3. The deformation of the structure mainly focused on the isolated layers and the superstructure shift as a whole. the interlayer displacement of the isolation system is far low. the earthquake effect of the base - isolated structure is dicided on the horizontal stiffness and elastic and plastic capability of the isolated layer, the base shear, the interlayer shear, th ' e interlayer displacement of the isolation system is far lower than the result of the base - fixed system under the same condition of the superstructure

    橡膠墊隔震結構在水平地震作用下的反應以「整體運動」為主，結構位移主要發生在隔震層，上部結構的層間位移很小。隔震結構的地震反應主要決定於隔震層的剛度和彈塑性性能。其中，隔震結構的基底剪力，上部結構的層間剪力在多遇地震下，比不隔震時均有所降低。
  4. Technology, as a kind of knowledge, affects humanities and arts. media technology is not isolated. it exists in a four - layer structure, namely customer layer, technique layer, organization foundation layer and social system layer

    當今時代，媒介技術高速發展，社會環境影響式微，盡管媒介技術無法撼動文化的深層結構，但是這並不能改變跨文化傳播向更深更廣發展的總趨勢。
  5. Analysis of the maximum displacement of isolation layer for base - isolated buildings with rubber bearings

    建築隔震橡膠支座的安裝質量控制
  6. Isolated structure system changes the energy dissipation mechanism of traditional anti - seismic structure, i. e. the earthquake energy is absorbed and dissipated by energy dissipative device set in a isolation layer rather than by the structure and structure elements. this excellent energy dissipation characteristic can not be realized by traditional anti - seismic structure

    隔震結構體系改變了傳統抗震結構體系的耗能原理，即通過隔震層的耗能裝置、而不是結構和結構構件來吸收並消耗地震能量，這種優越的耗能特性是傳統抗震結構體系所不能夠實現的。
  7. We used the icr and kunming mice and got the embryos of 3. 5dpc by means of superovulation, then cultured the embryos on the feeder layer derived from icr or kunming mice, dispersed the inner cell masses ( icms ) or es cell colony in the appropriate time. in this period, we analized the effects of feeder layer, trypsin and embryos isolated from uteri of different varietal mice on the es cell lines

    以icr小鼠和昆明小鼠為實驗對象，採用超數排卵的方法獲得小鼠胚胎，培養在用絲裂黴素c處理過的icr小鼠和昆明小鼠mef細胞飼養層上，選取適當時間離散增殖的icm和類es細胞集落，分析了在小鼠es細胞建系過程中，飼養層細胞、消化液及不同品系來源的胚胎的影響。
  8. Elimination quot; information isolated island quot; - electronic government affairs overall frame and top layer design

    電子政務總體框架和頂層設計
  9. Seismic isolated structure system is a passive active control system, which is different from traditional anti - seismic structure system. through setting isolation layer, earthquake energy is absorbed and dissipated and earthquake response of structure is reduced, so that the safe of structure is ensured

    隔震結構體系是區別于傳統抗震結構體系的一種被動控制體系，通過設置隔震層來吸收並消耗地震能量，減小結構的地震反應，確保結構的安全。
  10. The horizontal stiffness of isolated layer is reduced, the effect of the base - isolated structure is clear. and the damping is enlarged to reduce the displacement of the superstructure

    隔震層的水平剛度越低，隔震效果越明顯，隔震層位移隨之增大，需增大隔震層阻尼來減小位移。
  11. The annealing also changes the grain morphology. the p grains from the amorphous layer are finer than in the other cases. silicide grains grow towards the substrate at high annealing temperature and finally shrink into isolated islands, thus deteriorating the silicide / si interface smoothness

    由於非晶的形成，使得退火后晶粒要經重新形核和長大的過程，所以在同樣的退火條件下，與未形成非晶的樣品比，硅化物顆粒要小。
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