isolated target 中文意思是什麼
isolated target
解釋
單個目標-
In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis
擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。 -
In order to understand the mechanism of mtx further and to investigate the genotoxic target organs, we studied the dna damage and the correlation with dose of mtx by using the alkaline single cell gel electrophoresis ( comet ) assay. liver, spleen, bone marrow, thymus, kidney, testicle, stomach and peripheral lymphocytes of mice were isolated at lh, 3h, 6h, 12h, 24h after 5mg / kg mtx intraperitoneal injection
為了進一步了解甲氨蝶呤（ mtx ）的作用機制，探測其作用的遺傳毒性靶器官，為應用mtx治療過程中的臨床監測和副作用防治提供理論依據，我們以小鼠為研究對象，用單細胞凝膠電泳技術檢測了mtx腹腔注射染毒1h 、 3h 、 6h 、 12h 、 24h后對肝、脾、骨髓、胸腺、腎、睪丸、胃和外周血淋巴細胞的dna損傷作用及損傷程度與mtx劑量間的關系。 -
Then the research of image enhancement based on of plateau equalization algorithm and genetic algorithm is done then in this chapter. image segmentation with algorithm of 2d - maximal variance between - class and region growing is accepted to eliminate the background and the isolated noise points which can not be the target
論文在紅外目標檢測方面，以對經過預處理的圖像進行分割為切入點，採用二維類間方差法和局域生長法來進行圖像的分割，消除了原始圖像中一些不可能為目標的孤立噪聲點和大面積的背景區域。 -
This paper carried through the detailed description and the discussion first to the new generation of operation strut system ngoss theory and its the system organization which proposed by telecommunication management forum tmf. and it unified the existing oss of chongqing tietong to carry on analysis, contrast and reorganization, then fully used the service process flows, public bus structure, public service data and the technical thought of ngoss module and so on. according the frame construction instruction of etom, carried on the analysis, the conformity and develop the useful and discard the useless on the foundation of the system in existence, constituted the new generation of operation support program tbss holistic frame and the system skeleton of tietong characteristic, conforming to the chongqing tietong actual situation, to guarantee the realization of enterprise strategic target according to tbss concentrated deployment and manages the key application system and the data gradually, insure realizing the complete sharing of information in the entire enterprise and the end - to - end process automation, broke thoroughly the information isolated island of the chimney
本論文首先對新一代運營支撐系統（ ngoss ）的理論及其體系機構進行了詳細的探討，並且結合重慶鐵通現有運營支撐系統進行分析、對比、整理，接著充分利用ngoss體系架構和理論中的業務過程流、公共總線結構、公共業務數據以及ngoss組件等技術思想，根據增強型電信運營圖etom的框架結構，在對已有系統的進行分析、整合和揚棄的基礎上，制定了具有鐵通特色、符合重慶鐵通實際情況的新一代運營支持系統tbss的整體框架和體系構架，以確保逐步根據該體系架構集中部署和管理關鍵應用系統和數據，實現全企業信息的完全共享和端到端的過程自動化，徹底打破煙囪式垂直應用體系而造成的信息孤島，保證企業戰略目標的實現。
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