laser confocal fluorescence microscopy 中文意思是什麼
laser confocal fluorescence microscopy
解釋
激光共聚焦熒光顯微鏡- laser : n 鐳射激光，受激發射光，激光；萊塞；激光器，光激射器 （ ＝ light amplification by stimulated emis...
- confocal : adj. 【數學】共焦（點）的。
- fluorescence : n. 熒光（性）。
- microscopy : n. 顯微鏡學；顯微鏡使用術。
-
Laser confocal fluorescence microscopy
激光共聚焦熒光顯微鏡 -
We used fission yeast schizosaccharomyces pombe ( s. pombe ), an unicellular eukaryotic organism, as research material. electroporation was adopted to load ca2 + fluorescent indicator into yeast cell and under the laser scanning confocal microscopy ( lscm ), we observed cytosolic ca2 + distribution and relative content as well as fluorescence intensity of gfp - cam in different phases of cell cycle of yeast cell. flow cytometry provided a way of determining the relative dna content of populations of fission yeast
本文以單細胞的真核模式生物裂殖酵母（ schizosaccharomycespombe ）為研究材料，通過激光掃描共聚焦顯微鏡觀察酵母細胞胞質內游離ca ~ （ 2 + ）的分佈及相對濃度，以及不同周期時相細胞中gfp - cam的熒光強度變化，並採用細胞流式法對酵母細胞的相對dna含量進行測定以確定細胞所處周期時相。 -
H2o2 generation in guard cells was examined by laser scanning confocal microscopy based on fluorescence probe h2dcfda. the fluorescence intensity in guard cells of wild type and ios5 was essentially the same before treatment
以h2o2熒光探針h2dcfda結合激光掃描共聚焦顯微技術直接檢測了保衛細胞內的h2o2產生情況。未加任何處理之前，野生型與los5保衛細胞內熒光強度幾乎相等。 -
Furthermore, when 10 imo il h2o2 ( final concentration ) was added to the suspension after above treatment caused little reduction in hpts fluorescence. the results obtained by laser scanning confocal microscopy suggested that addition of exogenous aba resulted in a rapid decrease in fluorescence in most cellular compartments of the guard cells
維生素c可部分逆轉低濃度h2o2 （ 10 - 5 ）所誘導的氣孔關閉過程：並且10 - 6mol l的dpi和103u ml的過氧化氫酶（ cat ）在一定程度上也逆轉了aba誘導張開氣孔的關閉。 -
Based on epidermal strip bioassay, microinjection, patch - clamp and laser scanning confocal microscopy in the experiments, we provided the first evidence that map kinases, including mek1 / 2 or p38 / hog1, plays an important role i n aba - or sa - induced h2o2 signal initial, amplification and specific targeting in response to stimuli in guard cells. aba - or h2o2 - induced vicia faba stomatal closure. was inhibited or reversed by the specific inhibitor pd98059 of mek1 / 2 ; the guard cells were pre - incubated or - microinjected by 10 umol l - 1 pd98059, aba could not enhance the fluorescence intensity of h2o2 probe dichlorofluorescein ( dcf )
在對照實驗中， aba誘導熒光迅速增高；單獨的pd98059 、 pd98059和aba共同處理氣孔時，保衛細胞內h _ 2o _ 2探針熒光強度沒有增高；將pd98059注射進入其中的一個保衛細胞，再以aba處理，使得兩個有同樣熒光基礎的保衛細胞熒光強度對比強烈；將pd98059顯微注射進入已被aba誘導dcf （ dichlorofluorescin ）熒光強度升高的氣孔保衛細胞，熒光強度下降，而沒有被注射一邊的保衛細胞中的dcf熒光強度不變。
分享友人