membrane electrophoresis 中文意思是什麼

membrane electrophoresis 解釋
膜電泳
  • membrane : n. 1. 【解、生】(薄)膜,隔膜。2. 〈古語〉(古文件的)(一頁)羊皮紙。-braneous , -branous adj. 膜(狀)的,膜質的;隔膜的。
  • electrophoresis : n. 電泳(法)。
  1. Our previous studies showed existence of apoplast cam in the plant cell and cam had many extracellular functions. so it supposed cam may be one of important extracellular polypeptides and trigger the intracellular signal transduction by binding the receptor. in this study, radiolabelled ligand is used to investigate the binding characteristic of cam and a. thaliana protoplasts. and chemical crosslinking is employed to explore binding proteins in the membrane. at first, ( 35 ) ~ s - cam was produced using ( 35 ) ~ s - labeled amino acid mixture in e. coli. sds - page and autoradiograph indicated high - purified, high - specific radioactivity ( 35 ) ~ s - cam was obtained. electrophoresis of ( 35 ) ~ s - cam is the same as that of unlabeled cam with ca ~ ( 2 + ) or egta ; a quatitive of protoplasts was prepared by enzymolysis

    首先,用~ ( 35 ) s標記的氨基酸混合物喂養工程菌成功地制備了~ ( 35 ) s標記的擬南芥鈣調素( ~ ( 35 ) s - cam ) , ~ ( 35 ) s - cam純度高、放射活度高、 ca ~ ( 2 + )與egta存在時的電泳行為與未標記cam相同,可作為一種高靈敏性的探針用於進行受體學分析實驗;用擬南芥種子誘導愈傷,通過酶解制備了大量原生質體。
  2. Cellulose acetate membrane automated electrophoresis systems

    乙酸纖維素薄膜自動電泳系統
  3. ( 2 ) the mortality of cp cell in 10 were increasing directly related with the time in the low temperature. after 120d some cp cells died in the way of apoptosis. most nucleus of cells were condensed, and chromosome was marginated beside the nucleus inner membrane, cell sizes were reduced, dna ladder showed in dna gel electrophoresis

    ( 2 ) cp細胞系在10低溫下細胞死亡率與時間成正比, 120d的細胞具典型的凋亡現象,即細胞核固縮、染色體邊集在核膜內側;細胞體積變小;瓊脂糖凝膠電泳上顯示特徵性的「梯狀」帶。
  4. Get the inclusion bodies 2 ) western blot analysis of fusion protein expression ( 1 ) electrophoresis ( 2 ) transfer proteins from gel to membrane ( 3 ) blocking ( 4 ) incubation with primary antibody ( 5 ) enzyme conjugate incubation ( 6 ) substrate incubation. 3 ) gst detection module with cdnb enzymatic assay 3 purification of gst fusion proteins 1 the denaturalization of inclusion bodies. 2 purification using glutathione sepharose 4b column wash matrix with 1 pbs, prepare a 50 % slurry for batch purification method, pack column with matrix slurry

    三、 gst一hbrp重組蛋白的純化1 .超聲破碎細胞,離心,上清和沉澱進行sds一page電泳分析2 .樣品處理提取包涵體,變性后,加人用pbs平衡過的以utal腸onesepb抓脫4b ,室溫下孵育3 .以utadtionesepharose4b柱純化以utad雲onesepharose4b柱的準備根據毛山lel ,決定純化所需的gll衛ta1如oneseph娜se4b的柱床體積,用預冷的1xpbs清洗cldtathionese戶, se4b ,得到50 %的基質
  5. The mechanisms of such treatment have been proposed as inhibition of proliferation and angiogenesis, as well as induction of differentiation and apoptosis, as has been tested by various in vivo and in vitro experiments. in our experiments, it has also been demonstrated that after the treatment of arsenic trioxide, the k562 cells has undergone major morphological changes, which included nuclear shrinkage, membrane bleb and scattered apoptotic bodies. dna gel electrophoresis also discovered that the typical " dna ladder " phenomena in the treatment group, while the control group showed the regular genomic banding

    我們在實驗中觀察到as _ 2o _ 3作用人紅白血病k562細胞后,細胞生長明顯變緩,部分細胞出現皺縮、染色質濃聚及胞膜起泡現象,部分細胞胞膜破裂,在其周圍有緻密的凋亡小體出現, dna電泳出現典型的凋亡「梯狀」帶,提示as _ 2o _ 3能有效抑制k562細胞生長,誘導k562細胞凋亡。
  6. Ten isozymes and proteins were investigated by cellulose acetate membrane electrophoresis, the phylogenetic relationship among four groups of microtus fortis was analyzed based on the alleles frequency at these biochemical loci

    應用乙酸纖維素膜電泳法分析了四類東方田鼠的10種同工酶及蛋白,並觀察了它們在這些生化基因位點上的基因分佈特徵以研究它們之間的遺傳關系。
  7. I and hindlll respectively. after the digested products were run via agarose gel electrophoresis and transferred into nylon membrane, the southern blot was carried out using the cdna of rubber tree etrl as probe. the result of the southern blot showed that a hybridization band ( - 3. 0kb ) turned up from the ecor i digested product and another band ( - 4. 8kb ) turned up from the hindi ]

    從橡膠樹pr107品系的嫩葉提取基因組dna ,用限制性內切酶ecor 、 hinofll分別酶切,瓊脂糖凝膠電泳分離並轉膜后,用克隆的橡膠樹etri基因的cdna片段作探針進行southern雜交分析,結果表明, ecor酶切在約3 okb處有一條雜交帶出現, hi 。
  8. These essential component, hemagglutination, toxicity, immunogenicity of mycoplasma hyopneumoniae strain xy - 1 membrane protein were studied. the range of membrane protein mw is from 14kd to 100kd by means of sodium dodecyl sulfate - polyacrylamide gel electrophoresis, and molecular weights of the protective antigens were 36kd and 64kd by sds - page

    本研究以分離豬肺炎支原體野毒株( xy - 1株)的膜蛋白為試驗材料,系統地研究了膜蛋白的分子量范圍、血凝性、毒性及免疫原性等。
  9. Isolate and determin the proteins of chicken serum by cellulose acetate membrane electrophoresis

    醋酸纖維薄膜電泳法分離測定雞血清蛋白質
  10. From the result of electrophoresis, it known that the different components of the enzyme system were expressed cooperatively. in order to study the essence of cellualase induction of different carbon sources, the extracellular, plasm - membrane - bound and intracellular cellulases were made to transform different soluble inducers, and the productions were analyzed by gc chromatogram. the results supported the assumption that cellobiose acted as the direct inducer or the metabolic analogue, b - gentiobiose from cellobiose acted as the true inducer through different metabolism ways in different strains

    制備細胞膜外、細胞膜、細胞內纖維素酶,用定位於這三部位的纖維素酶分別轉化底物,然後進行氣相色譜定性分析,從而探討了不同碳源之間的誘導本質,結果認為不可溶的胞外纖維素以纖維二糖為橋梁,遵循不同的代謝途徑,直接或間接地誘導了兩株不同真菌纖維素酶的合成。
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