mercaptoethanol 中文意思是什麼

mercaptoethanol 解釋
巰基乙醇破壞蛋白雙硫鍵的還原劑
  1. Subconfluent cultures of rat were maintained in dmem / 10 % fbs, 24hrs before neuronal induction, media were replaced with pre - induction media consisting of dmem / 10 % fbs / lmm beta - mercaptoethanol ( bme ), to initiate neuronal differentiation, the preinduction media were removed, and the cells were washed with pbs and transferred to neuronal induction media composed of dmem / serum - free media, 5hrs later, 40ul dmso was given to every hole containing 2ml each

    分別取第5代和第13代的mscs ，以8x10 cm 『濃度接種於六孔板中的蓋玻片上，制備細胞爬片，每孔加zinl培養液。達到80融合時，更換新鮮培養液，並在培養液中加人終濃度為lrnm的p一流基乙醇，誘導24小時， pbs洗滌，而後換成無血清的培養液。
  2. Then the inclusion bodies were solubilized in 8 mol / l urea - 100 mmol / l p - mercaptoethanol ( - me )

    將較為純凈的包涵體溶解於8mo比的尿素溶液中， 4放置過夜。
  3. 2. morphologic studies of hepatocytes in different nutritive elements medium. objective : this study was designed to determine if different nutritive elements, such as transferrin, insulin, nicotinamide, p - mercaptoethanol ( - me ) and hepatocytes growth factor ( hgf ) affected the morphology of the primary hepatocytes in short term culture

    目的：本研究在普通培養液中添加轉鐵蛋白、牛胰島素、煙酷胺、 p琉基乙醇和促肝細胞生長因子，觀察這些營養成分對原代短期培養的小鼠肝細胞的形態學的影響，旨在探討一種適用於原代肝細胞的培養方案。
  4. One 66kd band appeared except 44kd main band when go isozyme above was subjected to sds - page and ce - sds, indicating this go isozyme was similar to that from spinach leaves which contained 40kd and 66kd simultaneously. whether b - mercaptoethanol was added or not when go isozyme was subjected to in sds - page and ce - sds, 40kd main band and 66kd band still appeared, indicating two subunits were not linked by covalent disulfide. amino acid analysis shew that the ratios of basic to acidic amino acid of go isozyme and its 40kd acidic subunit were 0

    菜心go同工酶的sds - page和sds -毛細管電泳（ ce - sds ）顯示，該酶除了含40kd主帶外，還有很淺的66kd帶，和之前我們提出的菠菜go同工酶含40kd酸性亞基和66kd堿性亞基相似； sos - page和ce - sds電泳中，無論加入-巰基乙醇與否， go同工酶都只有40kd主帶和66kd淺帶，表明菜心go同工酶中40kd酸性亞基和66kd堿性亞基不是以共價二硫鍵相連；用制備性sds - page法獲得菜心go同工酶的40kd亞基，並和菜心go同工酶一起測定其氨基酸組成，該go同工酶及40kd亞基的堿酸性氨基酸的比例分別為0 . 66和0 . 54 ，表明40kd亞基可能是個酸性蛋白，而66kd帶則是個堿性蛋白。
  5. The eg cell culture media consisting of dmem medium supplemented with fbs, chicken serum, beta - mercaptoethanol, l - glutamine. hepes, chicken embryonic extract and cytokines etc. after 24 hours culture, the isolated pgcs were selectively attached on the gonadal stromall cells in the plates

    加入新鮮的eg細胞培養液（含dmem 、胎牛血清、雞血清、 -巰基乙醇、 l -谷氨酰胺、 hepes 、雞胚浸出液以及細胞因子等成分）培養24小時以後， pgcs開始部分貼附於共培養的生殖原基細胞上。
  6. These adult cells are self - renewing and multipotential. in experiment, we can see that beta - mercaptoethanol and sfm used in sequence can induce differentiation, each one act alone ca n ' t have same result

    （ neuron ? ikecells人免疫細胞化學染色結果：未誘導的細胞表達極其微量的nse ，無一抗對照組完全陰性，而誘導的細胞80以上表達n
  7. After 4 days of growth, the intact inner cell mass ( icm ) were separated with 0. 05 % trypsin - edta and replated on feeder layer in dmem containing 15 % serum, 0. 1mmol / l nonessential amino acids, 0. 1mol / l 2 - mercaptoethanol, 2mmol / l glutamine, 100 units / ml of streptomycin and 100 units / ml of penicillin. after 4 - 6 days 5 es cell colonies were selected and expanded in which alkaline alkaline phosphatase was detected

    （ 2 ）小鼠囊胚或內細胞的培養和es細胞的分離培養了156個不同品系的小鼠囊胚，經過3 ? 4天培養后，將增殖出來內細胞團用機械法結合胰酶- edta處理，離散后培養于mef飼養層上， 4 6天後有5例出現了es細胞樣集落。
  8. These proteases were different with the ones reported in past and it suggests that there may be more proteases associated in psii of chloroplasts of spinach. such as, one of them could be resistant to 1 mol / l nacl and be almost inhibited by 1 mmol / l dtt or mercaptoethanol

    比如，一種蛋白酶對1mol l濃度的nacl溶液不敏感，但是受到dtt和巰基乙醇的強烈抑制以及1mmol ledta的部分抑制，而且這種蛋白酶能夠降解18kd外周多肽，在較廣的ph值范圍內都具有活性。
  9. Effects of liver matrix or and - mercaptoethanol on cultured cells from schistosoma japonicum

    巰基乙醇對日本血吸蟲細胞影響
  10. The enhancing factors of eb formation were studied. fifty thousand es - d3 cells per milliliter were incubated in dmem - high glucose supplemented with 20 % fbs and 2mm glutamine. the influencing factors which included hyclone fbs and homemade green season fbs, feeder cell layer, culture containers and 3 - mercaptoethanol ( - me ) were investigated

    以5x10 』加的eso3細胞種入含20胎牛血清和zinm谷氨酚胺的高糖dmem中，觀察進口胎牛血清和國產胎牛血清、飼養層、培養器皿以及卜硫基乙醇對es士3細胞形成的4debs數量的影響。
  11. On seeking the inducing agent, scientist had done many works to seek the appropriate agent and protocols, sanchez - ramos, wood - bury, kohyama, reyes and verfaillie had their own methods and concentrate on the neuronal factors and traditional antioxidant such as beta - mercaptoethanol etc. and on the other end of the world, chinese scientists had exciting progresses using the chinese herbs as effective inducing agent

    在尋找誘導劑方面， sanchez - ramos ， woodbury ， kohyama ， reyes和verfaillie工作主要集中在神經因子和抗氧化劑如beta -巰基乙醇等。在地球的另一邊，中國的科學家在使用中藥的提取物誘導mscs分化成神經細胞方面也取得了很好的實驗結果。
  12. In my experiment, only beta - mercaptoethanol have the potency to induce mscs to neural cells, exclude the other antioxidant such as glutathione, ascorbic acid and vitamin e ( alcohol dissolved ), and using beta - mercaptoethanol and serum - free dmem medium as the main differentiation a - gent

    結果顯示，只有beta -巰基乙醇具備此種誘導能力，排除了谷胱甘肽、抗壞血酸、維生素e等抗氧化劑的作用。本實驗使用了beta -巰基乙醇、無血清的dmem培養液和dmso作為誘導方案，在誘導后，可見很多誘導產生的神經元樣和膠質細胞樣細胞。
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