molecular construct 中文意思是什麼
molecular construct
解釋
分子構想-
One of us ( shapiro ) began this research with the realization that the basic operations of certain biomolecular machines within living cells ? recognition of molecular building blocks, cleavage and ligation of biopolymer molecules, and movement along a polymer ? could all be used, in principle, to construct a universal computer based on turing ' s conceptual machine
這項研究的開端，是本文作者之一夏比洛意識到，細胞內某些生物分子組件的基本運作方式，像是辨認基本分子建構單元、切開和連接生物聚合分子，以及組件沿著聚合分子移動的方式，理論上都能以塗林的概念為基礎，建構普適的計算器器。 -
Random amplified polymorphic dna ( rapd ) markers were used to construct molecular genetic linkage map of a single tree ( p. taiwanensis hayata. )
採用rapd分子標記來構建黃山松單株樹（仙居磐黃）的分子遺傳連鎖圖譜。 -
In order to check if it is the aim gene, we devised pcr with a new pair of primer, sequenced and registered the product with registration number : af449446. moreover we forecast and analysis the primary, secondary, tertiary and quaternary structure of the three protein : osftszi, crftszi and crftsz2 which has already cloned by our team before. after that we construct ftsz molecular evolution tree to site them in
又將生物信息學技術同實驗技術相結合，針對ftsz保守區設計引物擴增出一條衣藻ftsz片段，進行est搜索、比對、拼接，最終克隆出新基因crftsz1 ；連同本試驗室曾經獲得的另一個衣藻crftsz2基因進行蛋白質的一、二、三、四級結構預測、分析及比較尋找進化線索，建立了ftsz蛋白的氨基酸進化樹作進一步的進化定位。 -
( 3 ) volume shrinkage of composite decreases greatly with wpu increasing, even can arrive a negative number, but thermal stability is still good. ( 4 ) toughening mechanism is that : the formation of high molecular linear amino formate construct is the toughening reason ; hydroxyl - terminated pu - prepolymer and pf react to produce chemical bond effect which participate cure ; carboxyl - terminated pu - prepolymer and pf form semi - ipn which make toughened pf occur maximum
（ 4 ）通過紅外光譜對pf / pu共混物分子結構進行的定性分析、以及掃描電鏡對沖擊斷面形貌進行的分析，可證明聚氨酯預聚物增韌酚醛樹脂的機理是：兩個體系都是由於高分子量的線形氨基甲酸酯結構而增韌；端羥基pu與pf在共固化中產生化學鍵效應，參與pf的固化；而端羧基pu則與pf形成半互穿網路而增韌。 -
In this study, we designed a pair of primers based on the sequence of the upstream and downstream of chicken il - 2 gene. about 600 bp chicken il - 2 cdna fragment was cloned from cona - stimulated chicken splenocytes by reverse transcription - polymerase chain reaction ( rt - pcr ) and was subcloned into puc18 vector. recombinant clone was demonstrated by restriction enzyme digestion and dna sequencing. next, we construct recombinant plasmid pproex ? t - il - 2. the cdna of chicken il - 2 gene was subcloned into bamh i / hind iii sites of vector. the recombinant plasmid pproex ? t - il - 2 was transformed into e. coli dh5a and the bacteria was induced with iptg. it was demonstrated by sds - page and western blot that a 18kda protein which was equal to chicken il - 2 protein in molecular weight was expressed in e. coli dh5a. the expression level was up to 30 % of the total bacterial proteins. the purified protein was used to prepare the antibody against chicken il - 2 protein
經酶切鑒定及dna序列測定，該基因為雞il - 2基因，其序列與sundick等報道的完全一致。在此基礎上，我們把雞il - 2基因亞克隆到大腸桿菌原核表達載體pproex ~ （ tm ） ht中，構建重組表達質粒並進行確證性序列測定，重組質粒測序結果表明將編碼雞il - 2成熟蛋白的基因正確地插入到原核表達載體pproex ~ （ tm ） ht的目的位點。重組質粒轉化受體菌dh5後用iptg於37進行誘導培養， sds - page和westernblot分析顯示，表達的雞il - 2融合蛋白分子量約為18kda ，表達的融合蛋白經薄層掃描發現目的蛋白表達量約占菌體蛋白的30 。 -
In this experiment hcv structural gene was amplified by polymerase chain reaction ( pcr ), and was inserted into baculovirus expression vector pfastbacl to construct a recombinant transposing vector pfbl - cee. the plasmid pfb 1 - cee was transformed into dh1 obac competent e. coli cells. high molecular weight dna was prepared from the overnight cultures from the selected e. coli colonies, which was recombinant baculovirus shuttle vector containing hcv structural gene, named bac - cee
本實驗用pcr擴增hcv結構區基因，克隆到桿狀病毒表達載體pfastbacl中，構建成重組轉座載體pfb1 - cee ，轉化dh10bac大腸桿菌感受態細胞，篩選陽性菌落，抽提大分子質粒dna ，獲得含hcv結構區基因的重組桿狀病毒穿梭載體bac - cee ，脂質體介導轉染sf9昆蟲細胞，出現細胞病變后，收集含有重組桿狀病毒顆粒的培養上消，重新感染sf9細胞，收集sf9細胞，進行12 . 5 sds -聚丙烯酰胺凝膠電泳，可見表達的蛋白條帶。 -
By using self - assembly of beforehand synthesised host such as cyclodextrin, crownether, cylcophane to construct monolayer lb - film, rotaxane, and tube scientists have report a lot of comprehesive nano - self - assembled supramoleculars of well defined structure and special function. calix arene is the third host after cylcodextrin and crownether which can complex with both cation ion and small molecular
其中用預先合成的主體如環糊精、冠醚、環瑤通過自集構築單層的lb膜、索烴、管道等復雜的有精確結構和特殊功能的納米級分子聚集體有大量的研究報道。 -
The human placental alkaline phosphatase ( hplap ) ( ec 3. 1. 3. 1 ) would be used more widely in molecular biology and elisa for its enzyme activity and heat resistant was higher than that of e. coli alkaline phosphatase ( eap ) and calf alkaline phospha - tase ( c1ap ). it is promising to construct the gene engineering pichia pastoris to product the hplap in a scale for the resource of hplap is limited
L ）的活性和耐熱性都高於常用的大腸桿菌堿性磷酸酯酶（ eap ）和小牛腸堿性磷酸酯酶（ ciap ） ，因而更適用於分子生物學和酶聯免疫領域，然而其米源受到限制，探討利用畢赤酵母尺屍astorjs構建工程菌進行規模生產鳳有） 』 『闊的前景。 -
The self - assembly of quantum structure is a new science concept, which unifies the chemistry and physics, organic and inorganic, and so on. the process using self - assembly to construct quantum structure in mesoscope will greatly promote the development of integrated circuits, microelectronics, nanoelectronics and further, to molecular electronics. the self - assembly of quantum structure is being one of the hottest fields in solid physics, material chemistry, and nanoscience
量子結構組裝是一新的科學概念，它統一化學與物理，有機與無機，在介觀尺度上實現集成，它的實現，將會大大地促進納米集成光電子學，納米集成微電子學的發展，是當前固體物理、材料化學中的活躍領域之一。 -
The purified products were cloned into pgem - t - easy vector successfully, the cloned plasmids were transformed into e. coli. tgl. the specific recombinant plasmid was identified by molecular weight, pcr and restriction endonuclease analysis. the results indicated that the resultant construct contained the gene of interest hn at right orientation of the insert
經瓊脂糖電泳檢測，將大小與預計分子量一致的片段純化后連接到pgem - t - easy克隆載體中，再轉化大腸桿菌tgi感受態細胞，得到的轉化子經分子量比較、 pcr鑒定和酶切分析篩選陽性克隆。 -
The specific recombinant plasmid was identified by molecular weight, pcr and restriction endonuclease analysis. the results indicated that the resuctant construct contained the gene of interest f. the positive cloned was sequenced by sanger ' s dideoxy sequencing method. the results demonstrated that the f gene included an open reading frame which was 1662bp in length and encoded a protein of 553 amino acid
經核苷酸測序，該基因含一個1662bp的開放閱讀框，編碼553個氨基酸，與國外報導的新城疫各毒株f基因相比，核苷酸序列同源性為88 . 3 96 . 4 ，氨基酸同源性為92 . 1 96 . 4 。 -
The purified production was cloned into pmd18 - t vector. the cloned plasmids were transformed into jm109. the specific recombinant plasimid was identified by molecular weight, pcr and restriction endonuclease analysis. the results indicated that the resultant construct contained the gene of ibdv a fragment at right orientation
經瓊脂糖電泳檢測，將大小與預計分子量一致的片段純化后連接到pmd18 - t載體中，再轉化到jm109感受態細胞，得到的轉化子經分子量比較、 pcr鑒定和酶切分析篩選陽性克隆，結果表明，得到的陽性重組子中含有a節段全長基因，插入到載體中的方向正確。
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