momp 中文意思是什麼

momp 解釋
主要外膜蛋白
  1. Lastly by using the technique of dot blot hybridization, the genome dna of chlamydia was detected with the probe of momp gene labeled with dig - 11 - dutp by using the way of random primer. the results showed the degree of sensitivity of the probe was 10 pg and other pathogens could not be detected by this probe. by comparing the diagnostic ways of nucleotide probe and fc, the technique of nucleotide probe were proved to have high sensitivity and speci fi city

    最後，用地高辛隨機引物法標記成momp基因核酸探針，斑點雜交檢測衣原體基因組dna ，靈敏度可達10pg ，且不能檢出其它病原體的核酸。將核酸探針法與補體結合反應法對衣原體感染的診斷進行比較，初步證明該探針具有較高的敏感性與較強的特異性。
  2. The results demonstrated that the momps were protective antigens and the momp - iscoms of aeromonas hydrophila could induce the host to mount satisfied immunity. a pair of primers were designed according to the published nucleotide sequence of a putative outer membrane protein gene ( omp ) of aeromonas hydrophila. with the specific primers, a target fragment about 1. 1kb was amplified from aeromonas hydrophila l316 via pcr. the target fragment was inserted into the linearized pgem - t easy vector

    根據已發表嗜水氣單胞菌的外膜蛋白基因omp的核苷酸序列設計引物，利用pcr技術，擴增、克隆了嗜水氣單胞菌l316的主要外膜蛋白基因（ momp ） ，經t a克隆，插入到pgem - t系列載體上，測序分析結果表明momp基因最長的開放閱讀框（ orf ）為1035nt ，編碼由344個氨基酸組成，分子量為36kda的主要外膜蛋白質（ momp ） 。
  3. The homologious comparison proved the cloned gene had 96 % homology to the sequence of the omp gene, and the alignment of the amino acid sequence was 98 %. the recombinant plasmid was constructed with the target gene and the expressing vector pgex - 4t - l and then was transformed into e. coli bl21 ( de3 ) the fusion protein was expressed under the iptg inducing condition, and exhibited about 62kda in size, very close to the predicted molecular weight of gst - momp. furthermore, the fusion protein was specifically recognized by anti - serum which raised against the major outer membrane protein of ahl316

    Sds - page電泳分析顯示誘導表達的基因產物分子量約為62kda ，與預測的gst -外膜蛋白重組融合蛋白的分子量極為相似， western - blot進一步證實，表達產物能被嗜水氣單胞菌l316主要外膜蛋白特異性抗血清所識別，產生明顯的染色條帶，說明所表達的基因產物與天然的外膜蛋白抗原性一致。
  4. The major outer membrane proteins ( momps ) of an aeromonas hydrophila named ahl316 which isolated from diseased eel and the referential strain ahtps - 30 were purified and used for preparing immunostimulating complexs ( momp - iscoms ). the immunotrial was carried out by injecting european eel peritoneally with 20ug of momp - iscoms per eel

    本研究選取鰻源嗜水氣單胞菌l316和參考菌株嗜水氣單胞菌tps - 30 ，分別制備了這兩株菌主要外膜蛋白免疫刺激復合物（ momp - iscoms ） 。
  5. Make all these together, it proved that the cloned gene represented the major outer membrane protein gene of ahl316, and the expressed gene products shared identical antigenicity with the natural main outer membrane protein. the studies on preparation and application of momp - iscoms of ah l316 provided a new approach to fish vaccinology. the successfully cloning and expressing the major outer membrane protein gene of ah l316 made it possible to describe this gene ' s function under a single factor level, and also provided technical support for developing an advanced gene engineering vaccine and subunit vaccine against aeromonas hydrophila

    鰻源嗜水氣單胞菌l316主要外膜蛋白免疫刺激復合物的制備與應用研究，對研製魚類疫苗學問題進行了新的初步探索；成功地克隆和表達嗜水氣單胞菌l316主要外膜蛋白基因為在單因子水平上研究嗜水氣單胞菌外膜蛋白的作用和免疫功能以及制備嗜水氣單胞菌基因工程疫苗和亞單位疫苗奠定技術基礎。
  6. According to the sequences of the conservative region of avain chlamydia momp genes reported by people abroad, one pair of primers were designed to amplify momp gene with pcr whose template was genome dna of this strain, then it was sequenced

    其次，根據國外報道的禽衣原體momp基因兩端保守序列設計合成一對引物，以提取的衣原體基因組dna為模板， pcr擴增、克隆了momp基因，並對其進行了測序。
  7. Momp gene is amplified by using pcr technologyfrom dna of l2 trachoma chlamydia. the pcr products are recombined with vectors of pmd18 - t. more over, the recombinant plasmids are colonged. the dna sequence analysis shows the insert fragment is momp dna

    本實驗採用pcr技術，從l _ 2型沙眼衣原體dna中擴增出momp基因，與pmd18 - t載體重組行t a克隆，並進行dna序列分析，證明所克隆基因為mompdna序列。
  8. For creating a reasonable displaying method, momp gene fragment and pbv221 are recombined oritationally they are transformed into e. coli jm109 ( de3 ). a expressing protein was got with momp antigen. this study establishes the foundation for advance research of momp

    設計一條較為合理的表達方案，將momp基因片段與pbv221定向重組轉化大腸桿菌jm109 （ de _ 3 ） ，得到具有momp抗原性的表達蛋白，為研究momp的生物學功能以及研製沙眼衣原體基因工程亞單位疫苗奠定了基礎。
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