monellin 中文意思是什麼

monellin 解釋
莫耐林甜蛋白
  1. Shake flask fermentation experiments showed that the expression of the intracellular vhb in the yeast strain pichia pastoris gs115 / ppic9m / ppic3. 5k improved monellin production. secreted monellin is about 0. 3g / l

    Vhb在胞內的成功表達，使重組畢赤酵母高甜度monellin的分泌表達量提高了20 % ，達0 . 3g / l 。
  2. Expression of monellin gene and vitreoscilla hemoglobin gene in the methylotrophic yeast pichia pastoris according to the monellin amino acid sequence and pichia pastoris preference codon usage, the monellin gene was designed and synthesized

    同時探討vgb基因在畢赤酵母中的功能，並應用於高甜度monellin重組酵母，解決畢赤酵母高密度發酵中存在的供氧不足的問題，進一步提高高甜度monellin的產量。
  3. The recombinant expression vector ppic3. 5kv with vgb gene was used to transform the pichia pastoris gs115 / ppic9m via electroporation, yeast strain pichia pastoris gs115 / ppic9m / ppic3. 5k containing the integrated monellin gene and vgb gene was obtained by g418 ( 0. 5mg / ml ) plate

    進一步通過電擊轉化將vgb基因整合到重組酵母gs115 / ppic9m的基因組中，獲得了由高甜度monellin和vgb兩個基因同時整合的重組酵母gs115 / ppic9m / ppic3 . 5kv 。
  4. Under the induction by methanol, the expressed monellin was secreted into medium at the level of 0. 25g / l. the gene ( vgb ) coding for the vitreoscilla hemoglobin ( vhb ) molecule was cloned and funtionally expressed in pichia pastoris. shake flask fermentation experiments showed that the presence of the intracellular vhb in pichia pastoris efficiently enhanced cell growth under oxygen - poor conditions

    同時，採用畢赤酵母偏愛密碼子，人工合成了透明顫菌血紅蛋白基因（ vgb ） ；並構建了vgb基因的酵母胞內表達載體ppic3 . 5kv ，通過電擊轉化獲得了能在胞內表達有生物活性vhb的重組畢赤酵母gs115 / ppic3 . 5kv 。
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