monoclonal protein 中文意思是什麼
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By sds - page and immuno - blotting, we found that a monoclonal antibody of anti - chick brain cytoplasmic dynein intermediate chain antibody could react with cytoplasmic dynein intermediate chain - like protein at 67 kda in lily pollen. under confocal laser scanning microscopy after immunoflurescence labeling, we found that the dynein intermediate chain - like protein appeared punctated and was co - localization partly with microtubules in cytoplasm of lily pollen tube
免疫熒光標記及激光共聚焦掃描顯微鏡觀察發現，類細胞質力蛋白中間鏈在百合花粉管中存在於顆粒狀細胞器上；免疫熒光雙標及激光共聚焦掃描顯微鏡觀察發現，百合花粉管中類細胞質力蛋白中間鏈和微管存在部分共分佈。 -
The determination of human thymidine kinase ( htk ) in human serum, which is a key indicator of cancers can give information for the diagnosis and treatment of the malign diseases. the protein a layer was first self - assembled onto the gold electrode surfaces of quartz crystals, the monoclonal antibodies were then orientedly immobilized through the specific binding between the fc terminals of the antibodies and the self - assembled protein a. with this sensor, the affinity constant of antigen - antibody binding was estimated to be 1. 85 106 l / mol according to the scatchard ’ s plotting method, which proved the high bioactivity of antibody. finally, an amplified piezoelectric immunosensor was designed to determine the htk in
實驗中將蛋白a吸附於鍍金壓電石英晶體電極表面，用於定向固定htk單克隆抗體，成功研製了檢測htk的壓電石英晶體傳感器，並基於標準scatchard繪圖法，計算出免疫反應的親和常數為1 . 85 106l / mol ，證明該單克隆抗體具有較高的免疫活性;同時基於酶催化沉澱技術，設計了的檢測htk的質量放大壓電石英晶體傳感器，該傳感器可在0 . 1 - 10ng范圍內對htk進行定量檢測，應用此傳感器成功地對5種癌癥病人血清中htk的濃度進行了測定，實驗結果為癌癥的臨床診斷與治療提供了參考。 -
After the recombinant plasmid pcdna3. 1 / ts87 was identified by digestion of hindlll and bamh i, it transformed into cos7 by lipofectamine. expression product was identified by immunohistochemical method, sds - page and western - blot. the immunocytochemistry result has shown that specific brown - staining grains were found in the cytoplasm of cells transformed by recombinant plasmid versus not seen in cells transformed by pcdna3. 1 or normal cells ; the sds - page result has revealed that a band about 3 8kb was found in cell lysis transformed by recombinant plasmid versus not in cells transformed by pcdnas. l or normal cells ; the western - blot result has showed that only the band about 38kd was recognized by sera from rabbit infected by t. s artificially and sera from rabbit immunized with soluble antigen of t. s and with protein expressed by ts87 gene and by a monoclonal antibody of t. s
通過細胞的免疫組化，細胞裂解物的sds - page電泳， westem - blot分析檢測目的基因的表達情況。免疫組化結果顯示：重組質粒轉染的細胞質中有棕褐色顆粒，而空載體轉染細胞及正常細胞無此現象；細胞裂解物sds - page電泳結果顯示：只有重組質粒轉染的細胞在約38kd處有明顯的蛋白帶，這與理論計算的ts87基因表達蛋白的分子量為38kd基本一致； western - blot分析結果顯示：約38kd的蛋白帶能夠分別被旋毛蟲感染兔血清，成蟲蟲體可溶性抗原免疫兔血清， ts87基因原核表達蛋白免疫兔血清（ ts87血清）以及一株具保護性的旋毛蟲單抗特異識別。 -
By sds - page and immuno - blotting, the monoclonal antibody of anti - chick brain cytoplasmic dynein intermediate chain could recognize the 67 kda protein in purified golgi apparatus fraction from lily pollen. subsequently by immuno - gold labeling and transmission electron microscopy, we found that the dynein intermediate chain - like protein bound mainly to the membranes of golgi - associated vesicles. statistics analysis of dynein intermediate chain - like protein on golgi - associated vesciles showed the nearly equal chance of distribution on either cis - or trans - golgi - associated vesciles
對分離純化的百合花粉及花粉管中高爾基體組分進行sds -聚丙烯酰胺凝膠電泳和免疫印跡發現，抗雞腦細胞質力蛋白中間鏈單克隆抗體在67kda處有較強的免疫交叉反應；進而通過免疫金標結合電子顯微鏡觀察發現，大多數類細胞質力蛋白中間鏈存在於高爾基體附近的囊泡膜上；統計結果表明，類細胞質力蛋白中間鏈在順面和反面高爾基體附近囊泡膜上的分佈機率大致相等。 -
The protein product of meq gene was highly expressed in the nuclei of recombinant baculovirus infected sf9 cells when using an anti - meq monoclonal antibody ( mcab ) 23b46 to run the immunofluorescence assay ( fa ) ; the expression quantity and if staining patterns differed with different times post - infection ( pi ). the results of western blotting and immunoprecipitation test showed there were two specific bands around 60 kd. the results of the study demonstrated that the baculovirus / insect cell system is effective to be used to express nuclear protein of virus
結果發現:本表達系統產生的meq蛋白可被重組痘病毒表達的meq制備的單抗23b46所識別;在感染細胞中， meq蛋白僅局限於細胞核內，而且隨著感染后（ pi ）時間的增加，具有從核質向核仁和核膜轉移的趨向; w已stemblot和免疫沉澱試驗均證實重組桿狀病毒感染細胞裂解物中出現有兩條大小約為60kd的特異帶。 -
In this experiment, to screen the epitope of e2 protein of classical swine fever virus ( csfv ) defined by the monoclonal antibody ( mcab ) all, which had been prepared in previous experiment, the mcab all was raised in mice and followed by purification, the concentration of protein was assayed by using the bca protein assay kit
本室利用e2基因疫苗制備了多株單抗，為e2的抗原表位研究提供了條件，我們以噬菌體隨機12肽庫分析鑒定了豬瘟病毒e2蛋白的抗原表位，為深入研究豬瘟病毒的抗原結構、制備更有效的診斷試劑和疫苗提供更多理論依據。 -
New york ( reuters health ) jun 20 - the proportion of plasma cells in bone marrow and the serum monoclonal protein leel at diagnosis influence the risk that smoldering ( asymptomatic ) multiple myeloma will progress to symptomatic disease, new research suggests
紐約路透通訊社健康欄6月20日訊：最新研究發現，骨髓血漿細胞和血清單克隆蛋白水平的比例會影響無癥狀性多發性骨髓瘤發展為有癥狀性骨髓瘤的風險。 -
Then protein crystal of eif - 5a was observed using atomic force microscopy. further, the polyclonal and monoclonal antibodies were gained from immunized rabbits and rats. hypusine - contained eif - 5a plays a role in cell growth and differentiation
用得到的重組蛋白分別免疫家兔和小鼠，獲得兔的抗血清和三株持續分泌單抗的雜交瘤細胞株，為進一步分析eif - 5a的功能提供了檢測手段。 -
The research on the application and preparation of monoclonal antibodies against human alpha feto - protein was awarded in 1997 a second prize of “ guangxi scientific and technological progress ”
抗人甲胎蛋白單克隆抗體的研製和應用研究1997年獲廣西科技進步二等獎。 -
Nogo protein expressed by cns myelin is regarded as a major repulsive factor for the failure of cns axon regeneration. the interest in this protein stems principally from the neurite growth promoting effect of a monoclonal antibody ( in - 1 ) rasied against ni - 250 which has been proved later to be a part of nogo - a
近年來認為髓鞘表達的ni - 250和ni - 35蛋白可能是cns軸突再生失敗的主要因素，用ni - 250作為免疫原制備的單克隆抗體in - 1可識別ni - 250和ni - 35 ， in - 1的體內研究結果奠定了ni - 250和ni - 35作為中樞神經再生中最重要的抑制因子的地位。 -
The recombinant pcr technic was used to introduce a linking peptide klgggg to the site between scfv single chain form of the monoclonal antibody sz - 51 specific for the glycoprotein gmp140 on activated platelet membrane and uk32 low molecular weight form of pro - urokinase, to make the scfv - linker - uk32 chimeric gene. this gene was cloned into the transfer vector pbacpak9, and cotransfected with bacpak6 bsu36i digest into sf 9 cells. the fusion protein was secreted into the medium. in the fifth day after the cotransfection, the supernatant of the medium showed 107 iu ml fibrinolytic activity, higher than 25 iu ml fibrinolytic activity of scfv - uk32. elisa showed that the supernatant had the binding activity to activated platelet. wastern blotting also indicated that the supernatant could bind to the monoclonal antibody of urokinase b chain
為了提高重組導向溶栓分子scfv - uk32的溶纖活性，通過重組pcr方法在編碼scfv與uk32的堿基之間引入編碼klgggg連接肽的堿基序列，並克隆到轉移載體pbacpak9上，通過與線性病毒dna bacpak6 bsu36i digest共轉染到昆蟲細胞sf 9內，進行表達。表達產物分泌到上清中，共轉染后第5d天用纖維平板法測得sf 9細胞上清溶纖活性達到107 iu ml ，比未引入連接肽的scfv - uk32的表達活性25 iu ml高。 -
Using the monoclonal antibody to rev and the anti - sera against the rev env gp90 - gst fusion protein. the molecular cloned virus was detected by ifa. we also amplified the gp90 from the cells infected with the molecular cloned virus by polymerase chain reaction. all these results indicated the recombinant plasmid containing the total rev genome cdna is infectious
對snv株前病毒全基因組cdna克隆進行酶切，將酶切產物分別克隆進puc18中，分別將各個亞克隆進行測序，按照酶切位點和已知的部分序列以及rev物理圖譜將測得的序列進行拼接，完成了rev全基因組序列。 -
Identification of the epitope of prrsv n protein monoclonal antibody with phage displayed random peptide library
蛋白單克隆抗體識別的表位 -
The development of monoclonal antibody against meq protein of marek ' s disease virus and its applications
蛋白單克隆抗體的制備及應用研究 -
Preparation and characterization of monoclonal antibodies against s1 domain at n - terminal residues 249 to 667 of sars - associated coronavirus s1 protein
端249至667的單克隆抗體的制備與鑒定
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