mrna in situ hybridization 中文意思是什麼

mrna in situ hybridization 解釋
mrna原位雜交
  • mrna : mRNA ＝ messenger RNA 【生物化學】信使核糖核酸。
  • in : adv 1 朝里，向內，在內。 A coat with a furry side in有皮裡子的外衣。 Come in please 請進來。 The ...
  • situ : 西楚
  • hybridization : 混成作用
  1. The more accurate localization of 3galt - l mrna expression in mouse brain as studied by in situ hybridization was in good agreement with the general expression pattern seen with northern blot hybridization. in newborn mice, the dense granular layer and the purkinje cell showed a strong signal

    我們發現在小腦中1型鏈結構的變化與邵galt一1的表達變化基本一致:在新生鼠小腦中， 1型鏈結構在蒲肯野細胞和顆粒層細胞中都有表達，而在成年鼠小腦中， 1型鏈結構僅在蒲肯野細胞中表達。
  2. Nogo - 66 receptor, ngr, cloned in 2001, is a leucine - rich - repeat glycophosphatidylinositol - anchored membrane protein which mediates nogo - 66 inhibition of axonal outgrowth. both the long acidic amino - terminal domain and the nogo - 66 fragment have strong neurite growth inhibitory activity suggest that nogo - a has at least two inhibitory domains. northern blot, in situ hybridization, western blot and immunocytochemistry analyses show that in addition to oligodendrocytes, nogo - a mrna and nogo - a protein are also expressed in neurons in developing and adult brain and spinal cord, nogo - a is also found in peripheral organs such as heart and testis

    Northernblot 、原位雜交、 westernblot和免疫組化結果證明： nogo amrna和nogo蛋白除了在cns的寡突膠質細胞中表達，還表達于發育階段和成年的腦、脊髓和外周神經節的某些神經元中，在外周組織如睪丸和心臟也有表達； nogoe在cns和pns以及多種外周組織中有廣泛分佈； nogo （除表達于腦和心臟外，在骨骼肌中有較高表達。
  3. 5 the expression of gm - csf mrna in the nscs was detected by in situ hybridization

    5應用原位雜交技術觀察神經一幹細胞gm一csfmrna表達。
  4. $ mx # ( 2bo2 ) ffr $ $ htet # $ nq9i & kk $ 4n8 $ $ 4ta scf mana $ ismffs whether s1eep deprivation cou1d induce neuron pro1iferation has not got definite conclusion so far. in the present research, we performed small pedestal s1eep deprivation method to remove rapid eye movement s1eep ( rems ). we observed the neuron pro1iferation and differential in immunohistochemistry and doub1e 1abeling iariunohistochemistry staining, and the expression of stem ce11 factor mrna in situ hybridization technique after s1eep deprivation in rat hippocampus

    本研究應用細胞增殖標記brdu cna免疫組織化學方法結合雙重標記兔疫組織化學技術，觀察睡眠剝奪對成熟大鼠腦內神經元增殖及分化的影響，應用原位雜交技術觀察了睡眠剝奪大鼠海馬神經細胞scf基因inrna表達的變化，對睡眠剝奪引起海馬神經細胞增殖的分子機製作了初步探討。
  5. Firstly, a reverse hemolytic plaque assay ( rhpa ) was used to scale testosterone secretion of leydig ' s cells in single cells level. sequentially, star mrna expression was detected with fluorescent in situ hybridization ( fish ) at the same cells

    本研究首先採用逆向溶血斑法，鑒定單個間質細胞睪酮分泌上的差異，然後，在被鑒定的同一間質細胞上進行原位雜交（ fish ） ，以便在單個細胞水平上確定間質細胞是否存在功能及star基因表達的異質性。
  6. During the embryogenesis the level of kyot mrna increases in 17 dpc and the distribution is as same as adult mice. immunohistochemical sabc and in situ hybridization analysis showed kyot was mainly located in leydig cells of testis of mice

    採用免疫組織化學sabc和原位雜交方法進一步發現， kyot在小鼠辜丸中有特異性的表達，且特異性分佈於睪丸間質細胞。
  7. In the present experiment studies, an acute traumatic model of lateral cortical impact was employed to study expressive changes of microtubule associated protein - 2 ( map - 2 ), cyclooxygenase - 2 ( cox - 2 ), glial cell line - derived neurotrophic factor ( gdnf ), caspase - 3 mrna and protein after brain injury in rats. immunocytochemical staining, western blotting, nucleic acid in situ hybridization with an oligonucleotide probe and computer image analysis were used to detect the dynamic changes of map - 2 mrna, cox - 2 mrna, gdnf mrna, and caspase - 3 mrna in the cortex after moderate traumatic brain injury ( tbi )

    本實驗從自行設計大鼠腦損傷落體打擊器開始，先行建立了一個便於觀察和施加處理因素、控制性好、重復性好的動物模型，選用30g擊錘從25cm高處下落，沖擊應力d為355 . 09kpa ，打擊大鼠右頂部，造成中等程度的閉合性腦損傷，從病理形態學、組織超微結構觀察及微管相關蛋白- 2 （ microtubuleassociatedprotein2 ， map - 2 ） 、環氧合酶- 2 （ cyclooxygenase2 ， cox - 2 ） 、膠質源性神經營養因子（ glialcellline - derivedneutrophicfactor ， gdnf ） 、 caspase - 3基因及蛋白表達的時間性變化，詳盡系統地闡述腦損傷后各指標變化的時間規律性及表達差異可能的形成機制。
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