mutated site 中文意思是什麼
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The results showed that the f fragment, 728bp in length, could be a new gene with a little homology to the genes coding for polyketide synthetase or fatty - acid synthetase and the b fragment, about 4kb in length, is inferred to have repeat sequences around tn5 insertion site, in which there is homology to the wa 314 right arm of the high - pathogeniciry island of yersinia enterocolitica. to reveal any pathogenicity of enterobacter cloacae b8 and its mutated strains b8b and b8f to animals, the experiment with mice was carried out
結果顯示， f片段長度為728bp ，與現有生物數據庫的blast比較分析，發現該序列僅有局部短於1oobp的區域與polyketide合成酶基因或與脂肪酸合成酶基因有低的同源性，推測為一新基因； b片段長約4kb ，序列拼接結果推測靠近tn5插入位點部位有重復序列，對b片段tn5遠端的部分序列進行blast比較，發現它與小腸結腸炎耶爾森氏菌的強毒力島有一定的同源性。 -
Phylogenetic tree indicates the rab protein may play a role in vesicular trafficking from endoplasmic reticulum to golgi body. two tga in eo - rabl were successfully mutated to tgc by site direct pcr procedure and the truncate eo - rabl was obtained
以此重組質粒為模板進行pcr定點突變，將eo - rab1基因中前兩個tga突變為通用半胱氨酸密碼子tgc ，獲得截短型eo - rab1基因。 -
Therefore, blys, its receptor or related antagonists may find medical utility in the treatment of b cell disorders associated with autoimmunity, neoplasia, or immunodeficiency syndromes. in this study, epo signal peptide sequence and hsblys gene were linked by soe method. the fusion product was cloned into eukaryotic plasmids. pcdna3, pcdna3. 1, pefneo, respectively. meanwhile, the epo signal peptide sequence was mutated so as to form a restriction enzyme cut site : bin i. thus the recombinant plasmid can be used as secreting plasmid expressing other gene
本實驗通過3 』端互補，進行引物延伸合成epo信號肽序列：信號肽和hsblys基因採用重疊延伸拼接法形成融合基因；融合基因分別插入pcdna3 . 0 、 pcdna3 . 1 、 pefneo真核載體：引物延伸合成信號肽時，利用亮氨酸同義密碼，將信號肽基因的倒數第二個密碼突變，在重組載體上的信號肽序列之後，形成bln酶切位點，使三種載體成為分泌表達載體。 -
900 bp promoter - directed gus expression was highly induced by sa and bth, while the 603 bp promoter, whether mutated or not, did not respond to sa and bth induction, which indicated that the element in response to sa and bth lied among 575 ~ 872 bp from transcription start site
全長900bp啟動子能夠應答sa和bth的誘導，而603bp長的啟動子無論突變與否對sa和bth均無應答，證明sa和bth的應答區域在克隆啟動子的轉錄起始位點上游575 872bp之間。 -
One of them was forecasted to produce transcript factor binding site in intron 6 of silky tyrp1. gel - shift result of this mutation proved that there is transcript factor binding site around the mutated site
經過預測，發現其中的一個snp可能使絲羽烏骨雞的內含子6中出現了新的轉錄因子的結合位點。 -
Furthermore, by inserting " anther box " element to the mutated area of two site - mutation promoters, another two promoters, ipmas and ipmal, were created. in order to study the chemical - inducible capacity of wild and modified pr - la promoters, a coding sequence of gus ( | 3 - glucuronidase ) gene was fused to their downstre am, and the chimeric genes were cloned into pbin ! 9 - based plant expression vector
為了檢測得到的啟動子驅動效率及誘導活性，將所得到的啟動子、定點突變啟動子和插入花藥盒的啟動子與gus基因連接，構建了6個植物表達載體，同時分別構建包含ipl barnase 、 ipml barnase 、 ipmal barnase嵌合基因的植物表達載體。
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