native protein 中文意思是什麼
native protein
解釋
天然蛋白質-
This involves an equilibrium between the native and denatured forms of the protein.
這涉及到蛋白質的天然形式和變性形式之間的平衡。 -
For each fedding treatment, the longitude and latitude of the root egg, the number of egg chambers with each developmental grade all changed in a parabola model. the results from native page indicated that there were two specific protein i. e. vitellogenin ( vg ) or vitellin ( vn ) in the female wasp of n. vitripennis
同一種餵食處理中，卵巢長度的時間動態幾乎都無變化； 1級卵子數、 2級卵子數和成熟卵子數的變化，是拋物線變化趨勢，即先增加，然後減少；基部卵子短徑和長徑也是拋物線變化趨勢。 -
Mass spectrometry of synthetic hw - ma and rgd - hw are in full agreement with those speculated theoretically, which proves the success of peptide synthesis and refold. on isolated mouse phrenic nerve - diaphragm preparations, hw - ma can block the neuromuscular transmission in 35 minutes or so ( l 10 - 5 g / ml ), its biological activity shows 73 % decrease comparing with biological activity of native hwtx - i. it proves t hat the protein engineering of synthetic chimera hwtx - i has gained success to some extent, although it did not achieve our expectations. thus it proved that hwtx - i can be using as natural scaffold for protein engineering. and also emphasized the importance of " local stereo circumstances " of activity site when the foreign activity site was transferred into a natural scaffold
濃度為1 / 1059 / ml的hw一ma突變體能可逆阻斷小白鼠隔神經書高肌的接頭傳遞，阻斷時一間為35min左右，與天然hwtx一i比較，生物學活性下降3一4倍，說明合成的突變體改造獲得了一定的成功，盡管與我們預期的目標有一定的差距，從而證明hwtx一i可以作為蛋白質工程研究的天然分子骨架，同時也強調了往天然分子骨架中轉移外源活性位點時維持活性位點「局部立體環境」的重要性。 -
The main concept of this work includes the following aspects : ( 1 ) based on the soft - docking algorithm which is improved upon wodak and janin ' s protein - protein rigid docking algorithm, we took seventeen protein - protein complexes for examples to study the method how to select the near - native structure from docking conformations
本論文的主要研究內容如下： 1採用本組在wodak和janin的剛性對接演算法基礎上改進的半柔性對接演算法，以17種蛋白質-蛋白質復合物體系為例，我們研究了如何正確有效地從對接采樣中挑選出近天然構象的方法。 -
Why is it so difficult to determine the native structure of a protein
為什麼很難決定蛋白質的自然結構？ -
What forces determine the unique native ( most stable ) structure of a protein
是什麼作用力決定了蛋白質的獨一無二的（最穩定的）自然結構？ -
The profile of 53. 1kda purified protein on sds - page was compared with the native molecular weigh of 106. 5kda, which indicated it was a dimer with identical subunits
Ca 』 『及ca 』 『結合cts處理則提高可溶性糖含量和蛋白質含量，降低粗纖維含量，增加產量。 -
Professor david wan of the department of biochemistry and his post - graduate student denis ip discovered the novel orange fluorescent protein in tube anemone. it is the first native orange fluorescent protein ever cloned, and has an emission maximum lined at the orange color region of the visible light spectrum
這種天然橙色螢光蛋白是由中大生物化學系溫志昌教授及他的研究生葉子明共同研製，取自一種能發螢光的管葵，是本地首個利用生物科技克隆的螢光蛋白。 -
Western blot analysis showed that rhpk - 5 ( recombinant human plasminogen kringle 5, rhpk - 5 ) protein was recognized by mab same as native hpk - 5. the result suggested that we obtained correct gene sequence of hpk - 5 and got the purified rhpk - 5. section ii : construction of pbv220 / hpk - 5 vector for obtaining high - level hpk - 5 expression system, the hpk - 5 gene was recombined with plasmid pbv220 to construct the vector of pbv / hpk - 5
Coli ）作為宿主，經sds - page分析，篩選表達量最高的菌株作為發酵用工程菌株；用western - blot方法鑒定hpk - 5因子的免疫學活性；用搖瓶發酵的方法，研究發酵培養基的體積（溶解氧） 、組成成份及誘導起始時間和誘導持續時間對目的蛋白表達量的影響，優化hpk - 5基因工程菌的表達條件。 -
Generally, the protein - protein docking procedure is composed of four stages : searching of the binding modes of the receptor and the ligand, filtering of docked modes to eliminate the irrational docked structures, optimizing the structures, evaluating the docked modes with the refined scoring function and ranking them to obtain the near - native structures
通常，蛋白質蛋白質分子對接包括四個階段：搜索受體與配體分子間的結合模式，過濾對接結構以排除不合理的結合模式，優化結構，用精細的打分函數評價、排序對接模式並挑選近天然構象。 -
Xylanase refers to a type of enzyme which can hydrolyze xylans into xylooligosaccharides and d - xylose. it broadly exists in microorganism and has wide commerical application in industrial processes, such as feed, paper, foodstuff, medicine and energy industries. the xylanase gene xynb encoding the native protein of xylanase from streptomyces olivaceoviridis a1 was cloned into pichia pastoris expression vector ppic9 a
我們將來源於橄欖綠鏈黴菌a1 （ streptomycesolivaceoviridisa1 ）的木聚糖酶基因xynb的成熟蛋白編碼序列克隆到畢赤酵母表達載體ppic9中，轉化pichiapastorisgs115得到重組子，實現了木聚糖酶基因xynb的高效表達，表達產物能有效分泌且具有正常的生物學活性。 -
The mutant protein were found to possess higher bioactivity than native standard protein
檢測改構后之hbfgf蛋白活性高於未改構蛋白。 -
The results of native - page showed that the male fish could synthesis vitellogenin after the injection of 0. 05mg g " ' bw of 17 - estradiol for two weeks. the average content of vitellogenin in ej exposed group was 690. 2ng ml - 1 by elisa, it was significantly different from male control group ( p < 0. 01 ), which average content was 10. 7 ng ml - 1, and it was also much higher than the female control group, which average content was 285. 5 ng ml - 1. compared with control male goldfish, both the ca2 + content and total protein content in serum of the e2 exposed male goldfish showed significant increase
電泳結果表明，經過2周的17 ?雌二醇暴露，誘導雄性金魚產生了卵黃原蛋白，並通過elisa檢測卵黃原蛋白的平均含量為690 . 2ngml ~ （ - 1 ） ，和對照組雄性金魚平均含量為10 . 7ngml ~ （ - 1 ）的差異極顯著（ p 0 . 01 ） ，比雌性對照組檢出量285 . 5ngml ~ （ - 1 ）高1倍多，並且17 ?雌二醇可以誘導雄性金魚血漿鈣離子和血漿總蛋白含量增加。 -
This newly discovered nutriceutical modulation of gsh by the use of humanized native milk serum protein isolate of bovine origin in aids and cancer may well find other applications in disease where oxidative stress and pathology of gsh metabolism are largely implicated
人乳化的牛乳乳漿蛋白分離物對谷胱甘肽的營養調節作用最初是用於治療艾滋病和腫瘤病人，目前發現還可應用於和氧化負荷和谷胱甘肽代謝異常有關的其他疾病。 -
Of the department of biochemistry and his post - graduate student, denis ip cloned the first native orange fluorescent protein from tube anemone
及其研究生葉子明，成功研究出首個橙色螢光蛋白。魚缸內正是用來提取螢光蛋白的管葵。 -
The sequence of amino acids is sufficient to determine the native state of a protein
蛋白質中氨基酸的序列就決定了它的自然狀態。 -
The positive transformants with the integrates mn - sod gene and cuzn - sod gene were identified by zeocin - resistance, pcr screening and expression in p. pastoris. the recombinant mn - sod protein and cuzn - sod protein was successfully expressed in pichia pastoris based on the evidences that the obvious activity of sod existed in native - page and enzymatic activity test
Pcr鑒定進一步說明，目標基因已經重組到宿主基因組染色體上； 0 . 5甲醇誘導表達后，活性電泳出現明顯活性條帶，重組酵母發酵液中mn - sod的活性約是對照菌株sod活性的2 . 6倍； cuzn - sod酶活力約是對照菌株sod活性的1 . 3倍。 -
The positive transformants with the integrates mn - sod gene was identified by zeocin - resistance, pcr screening and expression in p. pastoris. the recombinant mn - sod protein was successfully expressed in pichia pastoris based on the evidences that a relative molecular weight about 23kd appeard in sds - page, the obvious activity of sod existed in native - page and enzymatic activity test, and mn - sod activity was specific base on the inhibition with the mixture of chloroform - enthanol ( 3 : 5 / v : v ) and potassium cyanide. two secreted plasmids ppiczaa - sodm18 and ppiczaa - sodc were constructed and after there linearization were transferred into chromosome of pichia pastoris gs115 by electroporation
Pcr鑒定及mut表型分析進一步說明，目標基因已經重組到宿主菌基因組染色體上； 0 . 5甲醇誘導表達后， sds - page結果顯示，表達的蛋白相對分子量約為23kd ，活性電泳出現明顯活性條帶；酶活性測定顯示，重組菌株sod活性比對照提高5倍左右；氯仿-乙醇（ 3 : 5 v : v ）和kcn （ 5mmol l ）抑制反應進一步證明，所表達的sod為錳超氧化物歧化酶。 -
Sds - page and western blotting indicated that the molecular weight of the expressed protein is 43kda, similar to that of the native vde enzyme
隨著iptg誘導時間的延長，蛋白量逐漸增加，誘導4h后，它佔大腸桿菌總蛋白的25左右。
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