negative staining 中文意思是什麼
negative staining
解釋
背景染色法-
The his - tagged peacl - gfp purified from the supernatants could polymerize into green fluorescent filamentous structures with diameter, length and shape being identical to that of muscle f - actins, which could be labeled by tritc - phalloidin ( a specific agent for staining actin microfilaments ), and were identified as having a 9 nm diameter by negative staining, corresponding with that of the muscle f - actins ( 7 - 10 nm ). under polymerization conditions, his - tagged peacl - gfp polymerized with kinetics similar to those of skeleton muscle actin, that is, an obvious lag nucleation period at the beginning of polymerization and an s - like typical polymerization curve could be obtained. the critical concentration is 0. 75 umol / l near to that of chicken muscle actin ( 0. 56 umol / l ) under the same condition
熒光標記結合熒光顯微觀察表明：從可溶性上清中純化的his - taggedpeac1 - gfp聚合形成的微絲不僅可以直接在熒光顯微鏡下觀察，也可被微絲的特異標記物鬼筆環肽所標記，而且其直徑、長度以及形態上與已知的聚合肌動蛋白熒光絲一致；電鏡負染的結果進一步證實其直徑為9nm ，與傳統微絲直徑相當（ 7 ? 10nm ） ；聚合曲線有明顯的停滯期，為典型的s型聚合曲線，聚合臨界濃度為0 . 75 mol l ，這一結果與已有報道相似。 -
P - selectin was negative both in the normal skin and in the postmortem damage group. in the wound specimens aged 1h, we detected expression of icam - 1 in the epidermis, a strong positive staining was observed in 3 hours after wound, and continued to 1 days from the skin was injured after incised, weak staining were showed on epiderm of the injury margin after 3 days incised, and disappeared in 5 days. expression of icam - 1 was also detectable in polymorphonuclear cells ( pmns ) in the wound specim - ents aged 3h
本實驗研究證明，皮膚損傷后表皮層icam1表達增高，其表達呈現一定時間規律性，在損傷后lh ，損傷邊緣表皮細胞即呈黃褐色染色，傷后3h組， icam一二在表皮層中呈深黃褐色，並一直持續至傷后id ，傷后3d組icam 1染色轉淡，呈黃褐色染色，在傷后sd及7d組， 1 m一二表達基本恢復至正常狀態。 -
. moreover, some samples appeared several active bands in the gel, which indicated the existence of different types of sod or multi - subunits of sod in these samples. the bacterial strain 276 is a gram - negative rod bacterium and there are more than 3 polar flagella, which observed after the gram ' s staining and flagellum staining
同時，利用非變性聚丙烯酰胺凝膠（ page ）電泳后的凝膠顯色反應，發現一些樣品出現了多條活性帶，這可能是因為在這些細菌提取物樣品中含有不同類型的sod分子，或是同一類型的sod含有多個亞基組成。 -
In this thesis, the fist cyanophage isolated in china was investigated with regard to the molecular biological characters. techniques for the large harvest and the electronic microscopy negative staining of the cyanophage were established. the genome library was constructed
論文對我國淡水中分離得到的一株藍藻病毒（噬藻體）的某些分子生物學特性進行了研究，建立了其大量增殖與電鏡染色的技術，構建了基因組文庫，並對部分序列進行了測定與和分析比較。 -
Negative staining technique
負染色法 -
0 with a significant level of p < 0. 05. results in normal skin of rats, we detected expression of icam - 1 in the sebaceous gland, hair follicle and epidermis. in the postmortem damage group, diffuse weak staining or negative was observed in the skin appendage and epidermis
Icam人在正常皮膚的表皮層、毛囊、皮脂腺中呈淡黃色染色，由於皮膚經常接受各種外來刺激，需要相對較快的細胞更新，而在正常皮膚中出現icam l的表達，說明其在維持皮膚生物學穩態方面有重要作用。 -
The virus could multifly on the mdck cells. and induce regular cytopathic effect ( cpe ). observed by negative staining electron microscope, the virus could be seen with coroniform spike. in the ultrathin sections, the viruses were found to multiply and induce inclusion body forming in the cytoplasm
本研究從某動物園兩只死亡大熊貓的肝、脾等臟器分離病毒，經mdck細胞適應培養，獲得一株病毒（命名為dxmv ） ，在該細胞上可形成有規律的病變。
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