nitrogenase 中文意思是什麼

They clearly demonstrated that nitrogenase could catalyze the reduction of acetylene as well as n2

他們明確地證明，固氮酶能催化乙炔以及N2的還原。

Reduced nicotinamide nucleotides can function as reductants to nitrogenase.

還原的煙酰胺核苷酸對固態酶可以起還原劑的作用。

First, the morphology, physiology of seed, biological traits, geographic distribution, ecological and community characteristics, karyotypes, nitrogenase activity of root nodules and chemical constituents of e. mollis were reviewed, respectively

本文對翅果油樹的形態特徵、種子生理生化、生態學特徵、染色體數目、群落生態、生物學特性、固氮生物學以及礦物質和化學成分等方面研究進行了綜述。

Pcr amplification using 2 degenerate primers for nitrogenase fe protein gene was performed with chromosomal dna isolated from the 29 isolates. the result suggested that a nifh amplicon of 323 nucleotides was detected in 7 isolates and the 7 isolates are c4 c5 g1 g2, w5 t1 and t7. these pcr amplified fragments were cloned, and sequenced

首先利用芽孢桿菌中芽孢的抗熱性將土壤溶液在100沸水中煮10 - 15分鐘，然後用選擇性無氮培養基進行初篩得到29株菌落形態不同的菌株；接著用固氮酶結構基因nifh的特異性引物對這29株菌進行pcr擴增，結果表明其中7個菌株具有nifh基因，這7個菌株的編號依次為： c4 、 c5 、 g1 、 g2 、 w5 、 t1和t7 。

Effects of diverse environmental factors on the growth rate ( od4oo ) and nitrogenase activity ( ara ) of the strain w12 hi nitrogen - free culture were investigated in our experiments. the results implied that the strain w12 could easily adapt to different cultural conditions : it could use various carbon sources ( especially glucose, sucrose, malic acid, mannitol ), propagate quickly and fix nitrogen at a temperature range of 15 ? to 40 ? and at 25 - 35 ? for optimum, at a ph range of 4 to 8. 5, at a saline concentration range of 0. 01 % to 1. 5 % ; low nlv " concentration had little effect on its nitrogenase activity. ara could also be detected when it grow in the culture media with 5mmol / l ntv "

W12菌株對環境因子的適應性研究：無氮培養條件下，測定溫度、碳源、酸堿度、滲透壓對w12生長及固氮能力的影響，結果表明，在15 - 40下均能生長並表達固氮酶活性，其最適生長及固氮的溫度為25 - 35 ；能利用葡萄糖、蔗糖、蘋果酸、甘露醇等多種碳源生長並固氮，當培養基中同時存在蔗糖和蘋果酸時，細菌生長和固氮活性最強；在偏酸和偏堿的條件下（ ph4 . 5 - 8 . 5 ）均能保持較強的生長勢和較高的固氮酶活性，並能通過調節自身代謝平衡並適應環境的酸、堿性變化，使培養液趨于中性：能耐受較高的滲透壓，培養液中卜、 5 naci濃度對其生長和固氮酶活性影響不大，當naci濃度升至2時，菌株的生長勢及固氮酶活性才有所下降：低濃度的鉸對其固氮酶活性影響不大，在0

The paper reviews home and overseas research works on exhumation and use of nitrogen - fixing resource, genetics engineering of nitrogen fixation, and biochemistry of nitrogenase and chemical modeling of biological nitrogen fixation, and describe the prospect of biological nitrogen - fixing research

本文從固氮資源的發掘和利用、固氮的遺傳工程以及固氮酶生物化學和化學模擬生物固氮三方面對國內外的研究進展進行了全面的闡述，並對生物固氮研究的前景進行了展望。

In this thesis, a strain of associative nitrogen fixing bacteria with high nitrogenase activity was selected and the further studies for it were conducted, the results obtained are as follows : selection and identification of associative nitrogen fixing bacteria with high nitrogenase activity. the nitrogenase activities detected by means of the acetylene reduction method and growth rates tested by optical density of germ suspension under the x 400nm ( od4oo ) of all the strains tested except for the strain w12 reduced gradually due to frequent subculture during a year. however, the strain w12 has been showing high and stable nitrogenase activity and growth rate since it was isolated five years ago

固氮菌株的篩選與鑒定：測定從不同來源分離、收集和保藏的12個固氮菌株或分離物在不同保存時期的固氮酶活性和生長能力，發現多數菌株在初分離得到時固氮酶活性較高，但隨著轉接次數的增多卻逐漸喪失固氮酶活性，生長也隨之減弱，只有w12在五年前從埃及分離得到至今一直保持旺盛生長勢和較高的固氮酶活性， 48h內乙烯生成量可達1800nmol乙烯ml菌液以上，遠遠高於其它菌株，其生長量（ od _ （ 400 ）值）也遠遠高於其它菌株。

2. proceedings of the reseaches on the novel nitrogenase mnfe protein and crfe protein several new batches of mnfe protein and crfe protein were prepared. their substrate reduction activities have been determined by the complementation with uw45 fe protein

新型固氮酶mnfe蛋白和crfe蛋白的特性與結晶研究在已有的工作基礎上，分離純化了幾批mnfe蛋白和crfe蛋白，並用部分純的ugh fe蛋白進行活性互補，分別測定了mnfe蛋白和crfe蛋白的底物還原活性。

Hormonal regulation of plant growth regulator br and shk - 6 on soybean biomass and nitrogenase activity

6對大豆生物產量和根瘤固氮活性的激素調控研究

The nifh sequence similarity between isolates t7 and paenibacillus odorifer was 97 %. the 7 isolates with nifli gene were tested for their nitrogenase activity using acetylene reduction assay ( ara ). isolates c4, g2, t7 and w5 showed nitrogenase activity, whereas nitrogenase activity was not detected in the isolates c5, gl and t1 and suitable conditions for demonstrating nitrogenase activity for them remain to be found

採用乙炔還原方法對這7個菌株的固氮酶活性進行了測定，其中4個菌株具有固氮酶活性，菌株g2和t7的固氮酶活性相對來講比較高，分別達到660 . 0nmol乙烯mg蛋白和292 . 5nmol乙烯mg蛋白，但用同樣方法卻沒有測出菌株g1 、 t1和c5的固氮酶活性，它們的最佳固氮條件還有待于發現。

This n2 and h + binding and reduced model also developed our early proposed two - site h2 evolution model for nitrogenase : site i, n2 - dependent h2 evolution site, is provided by the central six - fe region of femoco, which is also provided the n2, c2h2, co binding site

結合的高檸橡酸傳入，其反應式為: zh + + 2e 』 ， hz ，此反應不受co抑制; 。心inl90一高檸稼酸一m 。是一條通向femoco的重要的電子傳遞途徑。