northern blot 中文意思是什麼

northern blot 解釋
北方點墨
  • northern : adj （superl northernmost） 北的，北方的，住在北部的；北方特有的；〈N 〉美國北部的。n 1 北方人 （...
  • blot : n 1 墨污，墨漬，污點，污斑。2 瑕疵；恥辱，污名。3 〈古語〉塗去，抹去。vt （ tt ）1 用（墨水等）弄...
  1. Northern blot analysis shows that a 3. 2 kb transcript specific for znf323 was expressed at high levels in the lung, liver, and kidney, while weakly expressed in intestine, brain, muscle, cholecyst, heart, and pancreas

    Northernbolt分析表明znf323基因在人類早期胚胎不同時期（ 18 25周）表達水平不同，在肺，腎和肝臟的表達水平較強，其它組織表達較弱。
  2. The more accurate localization of 3galt - l mrna expression in mouse brain as studied by in situ hybridization was in good agreement with the general expression pattern seen with northern blot hybridization. in newborn mice, the dense granular layer and the purkinje cell showed a strong signal

    我們發現在小腦中1型鏈結構的變化與邵galt一1的表達變化基本一致:在新生鼠小腦中， 1型鏈結構在蒲肯野細胞和顆粒層細胞中都有表達，而在成年鼠小腦中， 1型鏈結構僅在蒲肯野細胞中表達。
  3. Nogo - 66 receptor, ngr, cloned in 2001, is a leucine - rich - repeat glycophosphatidylinositol - anchored membrane protein which mediates nogo - 66 inhibition of axonal outgrowth. both the long acidic amino - terminal domain and the nogo - 66 fragment have strong neurite growth inhibitory activity suggest that nogo - a has at least two inhibitory domains. northern blot, in situ hybridization, western blot and immunocytochemistry analyses show that in addition to oligodendrocytes, nogo - a mrna and nogo - a protein are also expressed in neurons in developing and adult brain and spinal cord, nogo - a is also found in peripheral organs such as heart and testis

    Northernblot 、原位雜交、 westernblot和免疫組化結果證明： nogo amrna和nogo蛋白除了在cns的寡突膠質細胞中表達，還表達于發育階段和成年的腦、脊髓和外周神經節的某些神經元中，在外周組織如睪丸和心臟也有表達； nogoe在cns和pns以及多種外周組織中有廣泛分佈； nogo （除表達于腦和心臟外，在骨骼肌中有較高表達。
  4. Northern blot results show that nos. 66 - 1, 84, 89 - 1, 97, 108, 152, 175 and 233 have stronger signal in sp6 - tester than in sp6 - driver ; and no. 23 has weak signal only in sp6 - tester, nos. 94, 165, 172, 185 and 191 have similar hybridization signals in both sp6 - tester and sp6 - driver ; nos. 4, 17, 18, 28, 6 9, 101, 156 - 1, 157 - 1 and 183 do not reveal hybridization signals in both sp6 - tester and sp6 - driver ; the results of sequencing and blastn and blastx on ncbi indicate that no. 23 cdna ( 846bp ) has significant alignments with nicotiana tabacum mrna for elicitor inducible beta - 1 - glucanase nt - sube76, and arabidopsis thaliana clone 7119 for glycosyl hydrolase family 17 ( protein id : at5g55180. 1, supported by cdna : 7119, supported by cdna : gi _ l 87001 54 ) and arabidopsis thaliana beta - 1 - glucanase - like protein ( gi _ 2 1594590 ) ; no. 84 cdna ( 560bp ) has significant alignment with lotus corniculatus aspartate aminotransferase mrna ( complete cds length = 1685, gi | 2605931 | gb | af029898. 1 | af029898 ) for aspartate aminotransferase ; no. 89 - 1 cdna has significant alignment with arabidopsis tha

    與同源性最高的擬南芥類似晚期胚胎發生高豐度蛋白比較，二者都具有lea 2結構域、保守分泌蛋白cog5608結構域和低復雜度區，都具有pkc磷酸化位點、酪蛋白激酶磷酸化位點、 n十四酞化位點和酚胺化位點，所不同的是： （ ）在結構功能域上， 152全長cdna編碼的蛋白質序列中多了1個lea 2結構域、 l個保守分泌蛋白cog5608結構域和1個低復雜度區； （ 2 ）在功能位點上， 152全長cdna編碼的蛋白質具有酪氨酸硫酸化位點、多了l個酪氨酸激酶磷酸化位點和1個可能的天冬氨酸富集區，但沒有n糖基化位點； （ 3 ）擬南芥類似晚期胚胎發生高豐度蛋白的lea 2結構域具有顯著性（ e
  5. Northern blot hybridization showed that leacbf expression had the same tendency with the enzyme activity. and it showed that the transcription factor acbf maybe have some relation with the enzyme activity of pal

    表明在紫外光照射條件下， leacbf編碼的轉錄因子的變化趨勢與苯丙氨酸解氨酶（ pal ）活性的變化趨勢有一定的相關性。
  6. Northern blot hybridization showed that leacbf has well - distributed expression in different tissues of wild tomato ( pink stage ). treatment the seedling with uv made the enzyme activity of the pal increased gradually and then decreased

    用不同的紫外線照射時間處理番茄幼芽，苯丙氨酸解氨酶（ pal ）的活性呈先是增加，達到最大值后再減小的趨勢。
  7. Northern blot hybridization showed that leerfl, leerf2 and pti4 ( a member of erebps in tomato ) expressed differentially in different parts of wild tomato fruit during ripening, which was related with fruit ripening

    Northern雜交分析表明： leerf1 、 leerf2 、 pti4 （已發表的番茄erebps成員）基因在普通番茄果實的成熟過程及果實的不同部位都有差異表達，表現出與成熟進程的相關性。
  8. Northern blot analysis showed that the mrna level of p27 k ' pl had no changes during ir responses ; the level of ubiquitinated p27klpl increased following 10gy - irradiation ; mg - 132 ( proteasome inhibitor ) inhibited the degradation of

    我們通過初步的研究結果揭示: northem檢測結果表明ir對p27kip 『 mrna水平無明顯影響; ir誘導p27kip 』泛素化水平的增加;蛋白酶體抑制劑（ mg一1
  9. Northern blot results suggested hal32 is a late gene and produced multiple transcripts in different sizes. to elucidate its function, hal32 was expressed as a gst - fusion protein in e. coli

    No ， themblot結果表明hai32是一個晚期基因，在病毒感染后72d時產生多個轉錄產物。
  10. Northern blot showed that kyot was expressed in testis in all stages of development and the level of kyot mrna began to increase in the adolescence and was the highest in adult

    Northern印跡顯示kyot在各發育雌小鼠睪丸中均有表達，橄掃描分析其表達水平在青春期迅速升高，在性成熟期最高
  11. In order to study the expression of 3 - defensins in liver as acute phase response proteins, a murine systemic acute phase responsive model was established by intraperitoneal injection o f lipopolysaccharide ( lps ) in our study. the mbd3 cdna sequence ( 145 - 169 bp ) was labled with [ - 32p ] atp as a probe to detect mbd3 mrna in different tissues by northern blot and analyze the time - and dose - dependant expression caused by lps. the 5 " flanking sequence ( - 167 - 179 bp ) of the mbd3 gene was designed as the probe and labled with [ - 32p ] dctp to investigate the binding of transcriptional factors to this region by electrophoretic mobility shift assay ( emsa ) and south - western blot

    以小鼠mbd3基因145 ? 169bpcdna序列合成探針，經[ - ~ （ 32 ） p ] atp標記后通過northernblot方法檢測mbd3在肝臟中的表達，同時分析了mbd3基因誘導表達的組織特異性，劑效和時效關系；結合mbd3基因啟動子區序列分析，以- 164 ? - 179bp雙鏈dna序列合成探針，經[ - ~ （ 32 ） p ] dctp標記后通過電泳遷移率改變實驗（ emsa ）和south ? westernblot方法對參與mbd3在肝臟中誘導表達調節的轉錄因子進行分析。
  12. 2. effect of nacl treatment on the subunits expressions and activities of h + - atpase and h + - ppase from suaeda salsa northern blot analysis indicated that the transcription of v - h + - atpase subunits b, h and c were obviously up - regulated by nacl stress. the transcripts of mrna of b and h subunits in 400 mmol / l nacl - treated plants were significantly higher than those in control during 0 - 120h and the mrna levels of b, h and c subunits were also increased under 100 and 400 mmol / l nacl treatment

    400mmol lnacl處理鹽地堿蓬植株0 - 120h發現鹽脅迫明顯誘導了鹽地堿鹽脅迫下鹽地堿茨液泡膜質子泵衷達分析及過量系達印mhxaw對擬甫芥耐鹽性的影響蓬液泡膜h 」 atpaseb 、 h亞基基因表達； 100 、 400mmol lnaci處理鹽地堿蓬植株，分析其葉片液泡膜h 」
  13. We further performed northern blot hybridization to reveal the regional distribution of 3galt - l transcripts in mouse brain. in cerebral cortex, p3galt - l was expressed abundantly in newborn mice with a slight decrease in adult mice, and a similar expression pattern could also be detected in hippocampus

    我們發現：在大腦皮質和海馬中， 3galt - 1的表達水平在發育進程中都相對較高；在間腦和腦干中， 3galt - 1的表達水平則較低；而在小腦中， 3galt - 1在新生鼠中表達很高而在成年鼠中表達明顯下降。
  14. The full length cdnas of 17 - hsd1 and 17 - hsd3, 17 - hsd8 were obtained by library screening and race, respectively. expression patterns ( tissue distribution ) of three types 17 - hsds were checked by rt - pcr and northern blot. the recombinant constructs of 17 - hsdl / pet blue2 and 17 - hsd8 / pcdna 3. 1 were made and subsequently transformed into the corresponding host expression cell of ( de3 ) placi and mammalian hek 293 cell

    首先從genebank下載在其他脊椎動物中已被克隆17 - hsd1 ， 17 - hsd3的氨基酸和核甘酸序列，並在序列保守區域設計簡並引物，然後分別以羅非魚卵巢和精巢cdna為模板進行rt - pcr擴增得到17 - hsd1 ， 17 - hsd3的中間片段。
  15. Dot blot and northern blot studies revealed that amphihmgb expression is restricted mainly to ovaries and guts in adult amphioxus while it is highly expression during embryogenesis

    文昌魚amphihmgb蛋白不含c ?端酸性尾巴，為一堿性蛋白，等電點為9 . 88 。
  16. Northern blot and rt - pcr analysis show that sh2a gene is ubiquitously expressed in many tissues with three transcripts. the aberrant expression of sh2a gene in some cancers was found. it suggests that sh2a gene relates to tumors

    Rtpcr及northern印跡雜交發現sh人在多種組織中有表?二?達，有3個轉錄本，而且在癌及癌旁組織的比較中發現，腫瘤組織中表達較高，初步證明與腫瘤的發生有關。
  17. 61 of these clones have hybridization signals on the x - photos of the reverse northern blot. then northern blots were performed for 23 of these 61 clones : nos. 4, 17, 18, 23, 28, 66 - 1, 69, 84, 89 - 1, 94, 97, 101, 108, 152, 156 - 1, 157 - 1, 165, 172, 175, 183, 185, 191 and 233

    其結構功能域中包含1個28個氨基酸殘基的信號肽ol2個晚期胚胎發生高豐度蛋白lea毛結構域（ 56 206 ， 205 323 ） 、 2個保守分泌蛋白cog5608結構域（ 72 200 ， 174 281 ）和3個低復雜度區門， 4265 ， 329習44其h級結構中存在有大量的捲曲螺旋區。
  18. During a systemic acute - phase response resulted by intraperitoneal injection of lps the expression of mbd3 gene was only detected in mouse liver and not in heart, lung, spleen and kidney by northern blot

    Lps刺激后引起小鼠急性時相反應。 northernblot檢測mbd3基因僅在肝臟中有表達，而在心、肺、脾、腎中無表達。
  19. Third, we examined the expression of ecbp21 dynamics during the incubation of angelica suspension - cultured cells and stress treatments through northern blot analysis. during the incubation of angelica suspension - cultured cells, the expression of ecbp21 gene increased from 0 to 12th day, and it reached a peak on the twelfth day. while the expression of cam gene increased from 0 to 9th day, and it reached a peak during the 6th - 9th day

    3 . ecbp21表達分析: northernblot分析表明:在白芷懸浮培養細胞生長周期中， ecbp21和cam都為組成型表達， 0一12天ecbp21表達量逐漸增加，第12天達到最高，隨后12一21天， ecbp21表達量稍有下降，維持在一個比較穩定狀態;而o一6天時， cam表達量逐漸上升，第6一9天達到最大表達量，隨后， 9一21天， cam表達量有所下降。
  20. Northern blot analysis showed higher expression level of pds gene in the stigma and anther than in the leaf and stem. the information we have got is very important for the further research of gene structure, expression and function of phytoene desaturase

    此基因的克隆，為深入研究八氫番茄紅素脫氫酶基因結構、表達和調節機制及其結構和功能的關系奠定了重要的基礎。
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