nucleic acid sequence 中文意思是什麼

nucleic acid sequence 解釋
核酸序列
  • nucleic : adj. 核的。 nucleic acid 核酸。
  • acid : adj. 1. 酸味的。2. 【化學】酸的，酸性的。3. 〈比喻〉尖酸刻薄的，易怒的。n. 1. 酸味物。2. 【化學】酸。adv. -ly ,-ness n.
  • sequence : n 1 繼續；接續；連續。2 順序；程序；次第；關系；關聯。3 後果；結果；接著發生的事；後事；後文。4 ...
  1. In this study, iltv - nm98a strain and iltv - wanggang strain were multiplied in chorioallantois. a pair of primers were devised according to the nucleic acid sequence of iltv tk gene and the dna of multiplied virus was used as pattern to amplify the gene of tk by polymerase chain reaction ( pcr ). the product of pcr was linked with suitable plasmid. then, the recombined plasmid was converted to escherichia coli. the converted escherichia coli

    根據已發表的iltvtk基因的核苷酸序列設計一對pcr引物，以增殖的兩株iltv的dna為模板，分別對它們的tk基因進行pcr擴增。將回收的pcr產物連接到適當的質粒載體上，轉化感受態大腸桿菌，通過篩選對iltvtk基因的陽性克隆進行擴增培養。
  2. Nucleic acid sequences of azoreduclase were searched and blasted in genbank. a pair of primers based on the conserved regions were designed. a specific fragment was amplified by pcr from the plasmid of rhodopsedomonas palustris and sequenced. the sequence contained a complete 471bp orf ( open reading frame )

    脫色實驗證明沼澤紅假單胞菌（ rhodopsedomonaspalustris ）對偶氮染料有較強的降解能力，我們通過genbank搜索，對所獲得的所有偶氮還原酶基因在ncbi進行比對並設計引物，從沼澤紅假單胞菌質粒中擴增獲得了一條含471bp完整開放閱讀框架的序列。
  3. The phylogenic relation and similarity of the gene is full length of lfy - like gene, which is 99 % similar both in nucleic acid sequence and amino acid sequences with that of ginlfy f rom the female. compared with the ginlfy of female, the gene from the male tree is deficient in 3 bp, and mutantion sites exists on the varied region of lfy - like

    該基因與文獻報道的銀杏雌株leafy基因相比，核苷酸序列同源性高達99 ，蛋白質序列同源浙江大學博士學位論文摘要性達99 ，缺少了3個核昔酸，突變均在植物leaf 』 y基因的非保守區內。
  4. Pairwise sequence alignment methods are concerned with finding the best - matching piecewise ( local ) or global alignments of protein ( amino acid ) or dna ( nucleic acid ) sequences

    雙序列比對方法涉及尋找（局部）最優匹配片斷或蛋白質（氨基酸）或dna （核酸）全局比對。
  5. The mechanism is that the introduced complementary oligonucleotides can bind to the corresponding mrna or double - stranded dna in genome and form partial double - stranded molecules or triple - stranded nucleic acid molecules by sequence - specific and nonsequence - specific antisense action, thus the target gene will be orientationally blocked and expression of the target inhibited so that therapeutic effect could be attained. in this study, we designed a fragment of human c ii ta cdna in antisense orientation using mrna of c ii ta as template. the primers were designed based on 94 - 500 nucleotides segment in 5 " end of ciita gene so that the interested gene contained 407 base pairs which included two aug codons in 1 16 and 188 nucleotides as well as the splicing site between the first and the second exons

    本研究設計以c tamrna為模板的反義cdna片段，從c ta基因5 』端第94位到500位核苷酸段設計引物，目的片段407bp ，覆蓋第116和188位兩個aug密碼子，也包含了第一外顯子和第二外顯子間的剪接位點：用常規分子生物學方法構建了反義片段的腺病毒表達載體（ padeasy - 1系統） ；腺病毒載體經hek293細胞包裝產生含反義片段的重組腺病毒，用氯化銫密度梯度離心法獲得純化的高滴度腺病毒；進行體外基因轉移，分別用反義片段真核表達載體轉染p388d1細胞和用重組腺病毒感染hela細胞，觀察導入的c ta基因反義rna抑制細胞內組成型或誘導型c ta基因表達的作用，從而達到調控mhc -類分子表達的目的。
  6. Blast search was used to characterize the sequence feature. the nucleic acid sequence of dseno gene had high similarity to that of other species

    以載體pcambia2301為骨架引入pbnzi中的名劉基因，構建了簡便、實用的中間載體pca州田ia230lg 。
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