nucleotide sequence analysis 中文意思是什麼

nucleotide sequence analysis 解釋
核苷酸序列分析
  • nucleotide : n. 【生物化學】核苷酸。
  • sequence : n 1 繼續；接續；連續。2 順序；程序；次第；關系；關聯。3 後果；結果；接著發生的事；後事；後文。4 ...
  • analysis : n. （pl. -ses ）1. 分解，分析；【數學】解析。2. 梗概，要略。3. 〈美國〉用精神分析法治療（＝ psychoanalysis）。
  1. In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis

    擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。
  2. G gene of rabies virus m, the of two main regions ( about 1000nt ), ranging from 3161nt to 4162nt and ranging from 4012nt to 4863nt of glycoprotein gene of rabies virus strain m, isolated from mouse in he nan, china were amplified by reverse transcriptase - polynerase chain reaction ( rt - pcr ) in order to complete glycoprotein gene of strain m. these regions were sequenced by the produce of pcr directly. comparison and analysis of nucleotide sequence and amino acid sequence deduced with that of other strains published was performed by computer with dnasisv 2. 5demo software

    本研究對我國河南某地野鼠體內分離的狂犬病野毒株mrv基因的3161位? 4162位（ 1001個堿基）和4012位? 4863位（ 851個堿基）片段進行了反轉錄pcr擴增和序列測定，得到mrv的糖蛋白基因全序列，用dnasisv2 . 5demo分析軟體，與已發表的代表性毒株g基因全序列進行核苷酸和氨基酸序列的比較分析，結果表明在同一基因型中， mrv和國際標準攻毒株cvs的同源性最高（ 96 . 5 ） ，和中國減毒株ctn的同源性最低（ 79 . 8 ） 。
  3. Determination and analysis of complete nucleotide sequence of swine vesicular disease virus

    豬水泡病病毒全基因組核苷酸序列的測定與分析
  4. Two complete squence primers were designed based on the result of race. a 1475bp sequence was amplified by pcr. the analysis of the product shows that the nucleotide and deduced amino acid sequences share 40 % - 60 % homologous to the corresponding parts of - glc gene family of pinus contorta, cucurbita pepo, arabidopsis thaliana by the blast _ w program comparison

    經blast搜索表明：克隆所得堿基序列和推導的氨基酸序列與已克隆出的小松樹、西葫蘆擬南芥等植物體內-葡萄糖苷酶基因的cdna相應序列有40 ? 60的同源性，因此我們推斷擴增所得到的序列為茶樹中-葡萄糖苷酶基因的cdna 。
  5. The cloning cdna fragment was extracted from positive clones and sequenced. the results showed that the cdna fragment was 816bp in size, encoding a protein which included 272 amino acids. the sequence homology analysis was carried out via the software blast 2. 0 network service in the four large databases - genbank, embl, ddbj, pdb, which had recorded 1 337 978 nucleotide and protein sequences. the results of the analysis indicated that the nucleotide homologous rates between the rubber tree etr and 15 recorded etrl of other plants ( mango, passion fruit, persia plum, strawberry, grape. . etc ) were 75 % - 80 % ; the protein homologous rates between the rubber tree etrl and these recorded etrl genes were 90 % - 95 %. from the results mentioned above, we could confirm that the cdna of rubber tree etrl had been cloned

    從陽性克隆子中提取克隆片段，經序列測定分析，結果表明，克隆片段的cdna大小為816bp ，編碼的蛋白質包含272個氨基酸。基因序列通過blast2 . 0networkservice軟體對genbank ， embl ， ddbj ， pdb四個大型數據庫中記錄的1337978條核酸和蛋白質序列進行序列相似性檢索，結果表明與芒果、一西番蓮、波斯梅、草毒、葡萄、西洋梨等15種已報道的植物的etrl基因cdnag的同源率為75 88 ；蛋白質氨基酸序列的同源率為90 95 ，表明本研究確實克隆到了橡膠樹etri基因的cdna序列。 4
  6. So must use information theory method depict and abundant the genetic diversity index system. in addition to, the introduce of molecule biology technology and the research of nucleotide sequence evolutive give a new method for population genetic, so must do deeply research about the analysis method of dna sequence data = the research main about the follows : there are three parts about the information model of population genetic : one about the shannon information entropy property of equilibrium population and the entropy change in the process of establish equilibrium ; another research is about the diversity measure - ment of genetic variation ; lastly, research the shannon information measurement about the disequilibrium gene variation. the result is : 1 to a definite gene distribution, the genotype entropy reach the maximum at the equilibrium population, the process of population from disequilibrium to equilibrium, the entropy get large and large

    此外，分子生物技術的介入及核苷酸序列進化的研究都為群體遺傳學的深入研究提供了新的途徑，但關于dna序列數據的分析方法需要作進一步的研究。本研究主要體現在以下幾個方面： （一）關于群體遺傳學的信息論模型研究，主要分為三部分內容：一是群體平衡的shannon信息熵的性質和群體平衡建立的熵變性質；二是群體遺傳多樣性測度的研究；三是非平衡群體的基因變異測量shannon信息量的方法研究。得到了如下結論： 1 、平衡群體的shannon信息熵最大，群體平衡的過程是熵的增大過程。
  7. Further sequence analysis show that only 6 base pairs of nucleotide and 2 amino acids are different between them. the homological cry3aa gene was expressed in escherichia coli. and the expressed products which contain a fused peptide of 66 - 97 kilo - dalton was observed by means of sds - page

    生物活性測定結果表明該菌株對榆藍葉甲（ pyrrhaltaaenescens （ fairmaire ） ）和光肩星天牛等鞘翅目昆蟲具有較高的毒力，因此初步確認該菌株屬于cry3類； （ 2 ）發現該菌株中編碼毒蛋白的基因位於質粒上，並且已經成功地克隆到該基因。
  8. It contained 187 amino acids deduced from the nucleotide sequence, and showed 65 % amino acid sequence identity with human aoeb166. the analysis of n - and c - terminal domains revealed amino acid sequences characteristic of features of mitochondrial and peroxisomal targetin

    原位雜交分析表明從受精卵到神經胚， aophihmgb基因廣泛表達，但隨著發育的進行，中胚層、內胚層和神經管的表達減弱，到1天幼蟲期，僅在表皮可檢測到表達。
  9. Benzyl chloride were used for extracting genomic dna of aspergillus. niger 14, about 1. 5kb specific fragment was obtained from genomic dna of aspergillus. niger 14 by pcr amplification with primers ( forward primer5 " ataggcatcatgggcgtctct3 " reverse - primer5 " cagctaagcaaaacactccgc 3 designed according to the known sequences of the phytase gene in the gene bank and pyrobest ? dna polymerase, after ligated with pmd18 - tvector, transformated into e. colidh5a competent cell successfully. 3. nucleotide sequence analysis of the cloned fragment revealed the presence of the whole phya gene in pcr product

    用氯化芐法提取了aspergillus . niger14 ~ #基因組dna ，根據genebank中已知的黑麴黴植酸酶基因序列設計出一對特異性引物（上游引物： 5 ataggcatcatgggcgtctc3下游引物： 5 cagctaagcaaaacactccgc3 ） ，採用pyrobest ~ （ tm ） dnapolymerase （高保真dna聚合酶） ，通過pcr方法從aspergillus . niger14 ~ #基因組dna中擴增出了預期的1 . 5kb左右的特異性產物，將其與pmd18 - tvector連接后，轉化e . colidh5菌株的感受態細胞，經質粒抽提、酶切鑒定，確認該目的產物已得到成功克隆。
  10. Complete sequence analysis of the two plasmids reveal that the small plasmid plp2000 with 2061 nucleotide encodes a putative 37 kda replicase. and in the downstream region of the replicase gene, it existed multi - tandem repeat of 17 bps

    Southernblot顯示plp2000和plp9000兩種質粒屬于滾環復制類型質粒，和滾環復制相關的鉆序列可能形成的二級結構分別為sso和ssou類型。
  11. An oligo - nucleotide primer was designed according to a conservative fragment of this gene of plants in genebank. two 3 ' - end coding sequences of this gene ( including complete sequences of exon 8, 7, 6, 5, 4 and partial sequences of exon 3 ), one, 1097bp, and the other 1109bp, were successfully isolated from brassicaeae napus and brassicaeae campestris. the dna sequence analysis shows that the two fragments are more than 90 % identical to orychophragmus violaceus, arabidopsis thaliana and brassicaceae napus

    同源對比分析已有的植物epsps基因序列，我們找到了該基因中一段極為保守的序列並設計了一條寡核苷酸引物，採用3 』 race法，從植物蜀雜9號油菜，白菜型油菜總rna中成功的擴增出了epsps基因3 』端編碼區（包含外顯子8 、 7 、 6 、 5 、 4的全部序列及外顯子3的部分序列） ，分別長1097bp ， 1109bp 。
  12. Then, the plasmid was transformed into jm109. the full length pstvd cdna recombinant plasmid was further identified by restriction mapping analysis and the nucleotide sequence of cdna cloned in pmd 18 - t vector was analyzed with ab1 377 dna sequence. test showed that the cdna of this chinese pstvd isolate was the same as pstvd - kf - 6 mild " type " isolate which originated from naturally infected potatoes in field of cornell university potato breeding program

    利用rt - pgr技術，設計一對引物，對田間採集的經鑒定含pstvd的陽性樣品進行全序列擴增，並將所得產物純化回收，連接到pmd18t - vector中，並轉化至大腸桿菌jm109中，挑選白斑進行雙酶切（ saci / ecorv ）鑒定證明插入片段為359bp大小，進行序列測定，所得克隆基因為pstvd全序列負鏈，大小為359bp 。
  13. Nucleotide acid sequence analysis indicates that two construts were correct

    對其進行了限制性酶切圖譜分析。
  14. Sars virus nucleotide sequence analysis based on signal processing

    病毒的核酸序列的分析
  15. Due to high homology of nucleotide between ba - dfe and subtilisin bpn, primers were designed and synthesized. the intact ba - dfe gene was amplified by pcr and cloned. the sequence analysis indicated that the ba - dfe gene has an open reading frame with 1146bp, which encodes 382 amino acid residues containing signal peptide, pro - peptide and mature peptide

    序列分析顯示，該片段的核昔酸序列中含有1146hp的開放閱讀框，可編碼382個氨基酸殘基的ba dfe前體蛋白，包括30個氨基酸殘基組成的信號肽、 77個氨基酸組成的前導肽和275個氨基酸殘基組成的成熟肽。
  16. The gene encoding the mature peptide was cloned from the total rna of h rhossiliensis owvt1 by rtpcr. sequence analysis of the gene was described in this papel the amino acid sequence, as derived from the nucleotide sequence of a cdna clone, had high homology with other subtilisin - like serine protease of nematogenous fimgi

    與其他絲氨酸蛋白酶基因序列比較表明，與其他線蟲卵寄生性真菌如paecilomyceslilacinus 、 verticilliumchlamydosporium及m . anisopliaevar . anisopliae同源性較高，而與捕食線蟲真菌arthrobotrysoligospora同源性較低（ 45 ） 。
  17. The gene cloning and sequence analysis of senv - d and senv - h isolated from china according to the published nucleotide sequences of sen viruses, specific primers were designed and synthesized. from the serum of two chinese patients with non - a - e hepatitis, one senv - d isolate named senv - d - bj1 spanning the complete coding region was amplified by semi - nested pcr, another isolate named senv - d - bj2 spanning the partial coding region ( including orf1 and orf2 ) was amplified too. from one blood donor serum, two senv - h isolates named senv - h12 - 1 and senv - h 12 - 2 spanning the complete coding region were amplified by nested pcr respectively

    Sen病毒d和h亞型中國分離株的克隆及序列分析我們在前期工作的基礎上，結合已發表的文章及基因序列，針對senv - d和senv - h基因組設計特異性引物，利用套式pcr技術從兩例non - a - e肝炎患者血清中分段克隆得到了一個senv - d亞型分離株（ senv - d - bj1 ）的全部編碼區基因序列，還得到了一個senv - d亞型分離株（ senv - d - bj2 ）的大部分編碼區基因序列（包括orf1和orf2 ） ；從一例健康人血清中分段克隆得到了兩個senv - h亞型分離株（ senv - h12 - 1和senv - h12 - 2 ）的全部編碼區基因序列。
  18. The special primers were designed and synthesized after the analysis to the nucleotide sequence homology of etrl recorded in genbank. the total rna extracted from latex was reverse - transcribed into first strain of cdna. the special amplification products ( cdna fragment ) were obtained from pcr when using the first strain of cdna as template

    通過對genbank登錄的已克隆的植物etr1基因序列進行核苷酸同源性分析，設計特異引物。以膠乳總rna為模板，反轉錄成cdna第一鏈，又以cdna第一鏈為模板進行pcr擴增，得到特異擴增片段。
  19. Phylogenetic tree analysis based on the genomic nucleotide sequence showed that the 14 csfv stains were divided into two groups. gxwz02, paderborn and 39 were classified into group 1, and other strains were classified into group ii. and the genetic relationship is very close between gxwz02 and paderborn

    系統發育樹分析表明，所比較的14株csfv被分為兩個群， gxwz02 、 paderborn和39這3個毒株被歸為群，其餘的11個毒株被歸為群， gxwz02與paderborn的親緣關系最接近。
  20. And pcr - rflp analysis was a sensitive, rapid and simple assay for the detection of nucleotide sequence change in ser 83. and our data sugget that pcr - rflp may be a useful device for detecting the mutation in gyra gene associated the resistance to fqns

    本研究通過試驗條件的篩選，建立了pcr - rflp檢測豬源致病性沙門氏菌gyra基因點突變的方法，可用於沙門氏菌氟喹諾酮類耐藥性的分子水平檢測和流行病學監測。
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