operon 中文意思是什麼

A group of related and closely linked structural genes, together with the operator gene that controls their expression are collectively called an operon.

一組相關的、緊密連鎖的結構基因加上控制其表現的操縱基因，統稱操縱子。

Trp operon and attenuationtrp

操縱子和弱化

Then the engineered genes of bio operon were carried on testing of the functional expression, and biow & biob gene were induced to express

而後對改造后的生物素操縱子基因進行了功能表達檢測及biow與biob基因的誘導表達。

Operon is a unit of bacterial gene expression and regulation, including structural genes and cis - acting control elements in dna recognized by regulator gene product ( s )

控制某一代謝途徑的相關基因，緊密連鎖地排列在一起，受同一操縱子控制。

14 chen x, su z, dam p, palenik b, xu y, jiang t. operon prediction by comparative genomics : an application to the synechococcus sp. wh8102 genome

我們同時比較了採用單個兩個及三個參數的模型，發現採用三種參數的模型總體效率敏感率特異率最高。

Used as 1. 1094 strain of bacillus subtilis as researched material, the structural genes of biotin operon ( bio operon ) were cloned, and its sequence were engineered

本研究以枯草桿菌asl . 1094菌株為研究材料，克隆了生物素操縱子基因，並對基因序列進行改造。

This experiment probed how to improve the expression efficiency of the structural genes of bio operon, and provided the feasibly researched route and scientifically theoretical foundation

旨在探索如何提高生物素操縱子基因的表達效率，為該操縱子基因的高效表達提供可行的研究路線和理論依據。

Used the genomic dna extracted by low melting - point agarose embedding method as pcr template, the full length of structural genes of bacillus subtilis bio operon were gained by long pcr method

將該方法提取的基因組dna稀釋100倍作為模板，採用長距離pcr方法，獲得了枯草桿菌生物素操縱子基因全長。

However, in the late phase of the growth, the strain hv grew a little faster than e. colihms174. hv ( ptz101 ) was constructed by the plasmid containing the phb operon and the parde gene tansformed into the strain hv

將帶有phb操縱子和質粒穩定分配基因parde的質粒ptz101引入vhb整合菌中，構建成為產phb的vhb整合菌hv （ ptz101 ） 。

The c - phycocyanin ( cpc ) operon of blue - green alga ( or cyanobacteria ) arthrospira platensis fachb341 was cloned, sequenced and characterized by using chromoseme walking method. the sequence includes cpcb gene ( 519 bp ), cpca gene ( 489 bp ), cpch gene ( 357 bp ), and upstream sequence of cpcb ( 427 bp ) and upstream sequence of cpch genes ( 184 bp ), 111 bp of phycocyanin intergenetic spacer ( pc - igs ). upstream sequence of cpcb gene was ligated into promoter - probe vector pegfp - 1 and transformed into three systems : e. coli, synechocystis pcc 6803 and a. platensis fachb341 by supersonic and electrophoresis methods

根據genbank中報道的節旋藻藻藍蛋白基因序列設計引物，首先克隆了鈍頂節旋藻（ arthrospiraplatensisfachb341 ）藻藍蛋白操縱子中亞基基因、亞基基因部分序列及二者之間的間隔區序列（ pc - igs ）並進行序列測定，然後根據此測序結果設計引物，通過染色體步移法克隆得到藻藍蛋白操縱子長度為2086bp的基因片段，其中包括藻藍蛋白亞基基因（ cpcb ， 519bp ） ，亞基基因（ cpca ， 489bp ） ，連接蛋白h基因（ cpch ， 357bp ） ，亞基基因上游啟動子序列（ 427bp ）以及各基因之間的間隔區（ pc - igs ， 111bp ； cpch與cpca間隔區， 184bp ） 。

Second, genomic dna polymorphisms of three mandarinfishes populations ( each bulked dna pool composed by 10 individuals ) were tested by rondom amplified polymorphic dna ( rapd ) using bulked segregant analysis ( bsa ). 12 primers selected from operon kitz yielded reproducible and polymorphic dna fragments ranging from 125bp to 3kb. these primers generated 60 scorable marker bands of which 15 ( 25 % ) were polymorphic in qiupu river population ; 59 bands in changjiang population, 15 ( 25. 4 % ) were polymorphic ; and 58 bands in wanfuhu mandarinfishes, 19 ( 32. 8 % ) were polymorphic

20個引物中有12個在三群體間產生多態性，這12條引物在秋浦河鱖魚中擴增出了60條帶，其中15條為多態性（佔25 ） ；長江鱖魚59條帶， 15條為多態性（佔25 . 4 ） ；萬佛湖鱖魚58條帶， 19條為多態性（佔32 . 8 ） 。

Contrasting analysis with the genes sequence of bacillus subtilis 168 strain ' s bio operon, the sequence of bio operon of as 1. 1094 strain had 12 bases difference with that of 168 train, and 7 bases caused variation of amino acid

1094菌株與168菌株bioafdb基因序列完全一致， 9個差異堿基是由於pcr擴增導致的。枯草桿菌生物素操縱子基因4個亞克隆序列的測序結果證實， asi

2. cloning of structural genes of bacillus subtilis bio operon diluted the genomic dna of bacillus subtilis as the template, long pcr product ( 10. 3kb ) and three salvage pcr products were separately gained by optimization of reaction conditions of pcr

枯草桿菌生物素操縱子基因的克隆將枯草桿菌基因組dna稀釋后，通過pcr反應條件的優化，分別擴增得到了生物素操縱子基因的長距離pcr產物（ 10 . 3kb ）和3個分段pcr產物。

Using pcr technology, a 2. 4kb dna fragment, part of tryptopanase operon, containing a promoter, a regulator gene tnac located downstream of the promoter and a desired tryptopanase gene tnaa which can be expressed by the promoter from e. coli k - 12, was cloned to pmd18 - t vector. the dna sequence is the same as which was published on science

為了證明質粒上的基因能表達出有活性的色氨酸酶，將這個dna片段克隆到pet28c質粒的bamhi和hind位點上，使該片段受t7rna聚合酶的啟動子控制，然後轉化噬菌體de3的溶源菌bl21 （ de3 ） 。

21 wang l, trawick j d, yamamoto r, zamudio c. genome - wide operon prediction in staphylococcus aureus. nucleic acids res., 2004, 32 : 3689 - 3702. 22 washio t, sasayama j, tomita m. analysis of complete genomes suggests that many prokaryotes do not rely on hairpin formation in transcription termination

本文所論述的不依賴終止子預測演算法被整合於我們開發的rnall局部二級結構預測軟體包內，用戶可免費下載，網址為http : digbio . missouri . edu wanx rnall 。

Puc18 - 169 was a subcloned plasmid containing the whole operon sequence of l - hydantoinase from arthrobacter bt801 which can convert 5 - benzylhydantoin to l - phenylalanine. hydantoin hydrolase which is responsible for the ring opening of hydantoin is one of the components of hydantoin utility enzymes of arthrobacter bt801. n - carbamoylase is a part of hydantoinase operon which can transform n - carbamoylamino acids into the corresponding amino acids

節桿菌（ arthrobacter ） bt801是由軍事醫學科學院生物工程研究所保存的l -乙內酰脲酶產酶菌株， puc18 - 169是由本室構建的含有節桿菌bt801的l -乙內酰脲酶完整操縱子序列的亞克隆質粒。

Similarity coefficient and genetic distances between two arbitrary individuals were caculated according to the data of experiments, the pedigree was constructed by two means ( they were upgma and parsimony ), and genetic diversity was discussedbased on selecting the best conditions of pcr with opg and opa primers from operon company, we obtained 163 bands using 24 random primers. in other words, 163 sites in crested ibis " genome were detected. the bands from 23 primers showed polymorphism. minimurn of polymorphic ratio was 0, maximum was 85. 71 %, and average was 48. 99 %. in the population which was composed by 37 individuals, most of genetic distances between two arbitrary individuals were less than 0. 1. the results showed that the degree of similarity among crested ibises was high while genetic diversity in the population was low accordingly

我們在對operon公司opg系列和opa系列引物進行優化篩選的基礎上，用24條隨機引物共擴增出163條譜帶，即檢測了朱?基因組中的163個位點，其中23條引物的擴增結果具有多態性；譜帶的多態比率最小為0 ，最大為85 . 71 ，平均為48 . 99 ；在由37隻朱?組成的種群中，任意兩個個體之間的遺傳距離絕大多數在0 . 1以下。這些結果表明，目前朱?個體間的相似程度很高，種群的遺傳多樣性水平較低。