orfs 中文意思是什麼

orfs 解釋
讀碼框
  1. A small cryptical plasmid pefr was isolated from enterococcus faecium strain df101. the complete sequence analysis of the plasmid show that it consists of 3176 bps, which contains four putative orfs. orf1 encodes a putative protein and is highly similar to repa which functions in replication

    從屎腸球菌df101菌株中分離到隱秘的小質粒pefr ，全序列分析顯示質粒pefr由3176bp組成，編碼四個推定的orf ， orf1編碼的一個推定的蛋白和復制有關的repa有很高的相似性。
  2. A mutant library of xanthomonas campestris pv. campestris ( hereafter xcc ) strain 8004 with 17820 clones was constructed by random transposon tn5gwsa5 mutagenesis, which cover 1800 predicted orfs of xcc genome

    用轉座子tn5gusa5誘變野油菜黃單胞菌（ xanthomonascampestrispv . campestris以下簡稱xcc ） ，構建了xcc8004tn5gusa5插入突變體庫。
  3. The insertion sites of tn5gusa5 were located by methods of tail - pcr and sequencing. for these 53 protease mutants, the insertion sites were distributed in 14 orfs

    運用tail - por和測序的方法定位了轉座子tn5gusa5的插入位置，經分析共插入14個orf中。
  4. The result of blastx shows that one orf is extracellular serine protease precursor gene ; 3 orfs function as regulatory genes ; 5 orfs are involved in secretion pathway ; 2 orfs are related to production of lps ; and the annotation functions of the other 3 orfs are not clearly related to extracellular protease prouduction. marker exchange method was used to study the relationship between the production of protease and the 3 orfs. a deletion mutant of xcc _ 4463 was constructed successfully

    Orf注釋表明，共中一個基因為胞外蛋白酶結構基因， 3個基因與合成調控有關， 5個與轉運分泌有關， 2個與lps合成有關，其餘3個orf的注釋功能與胞外蛋白酶的關系未見報道，為研究這3個orf與胞外蛋白酶產生的關系，採用同源雙交換orf缺失法進行了進一步驗證，成功地構建了xcc _ 4463缺失突變體，所得缺失突變體經檢測胞外蛋白酶減少，在寄主上致病性降低。
  5. Phaa, phab and phac were inserted to pbv - 220 with double digest of restriction enduonuclease. the expression vectors of pbv - a, pbv - b and pbv - c were constructed by orientaional cloning. indefication of expression vector with restriction enduonuclease digest showed that phaa phab and phac were in right orfs

    將phaa 、 phab和phac片段雙酶切后，定向克隆至原核表達載體pbv220 ，構建了三個原核表達載體pbv - a 、 pbv - b和pbv - c ；經酶切分析表明，所克隆的三個基因phaa 、 phab和phac置於表達載體的正確閱讀框架下。
  6. Using erase - a - base system, a series of exoiii progressive deletion mutants were prepared for sequencing dnd cluster. analysis of the sequence revealed that it contained three orfs

    為了對dnd功能區域進行序列測定和分析，通過exo缺失實驗，獲得了38個單向漸減缺失亞克隆。
  7. All of three orfs were demonstrated to be necessary for the dnd phenotype. for the survey of the biological relevence, different 5 "

    為了驗證這三個基因與dnd表型的關系，構建了一系列衍生於整合型載體pset152的質粒： phz2150 ， phz2151 ， phz2152和phz2153 。
  8. The derivatives of phz2104 resulting from the disruption of the three putative orfs respectively by aada can not confer dnd phenotype on zx1. using erase - a - base system, a series of exoiii progressive deletion mutants were prepared for sequencing of add gene cluster

    將add基因簇亞克隆到測序載體pbluescript sk （ + ）上，採用exo缺失突變法得到了66個單向遞減缺失亞克隆，然後對這些克隆進行測序。
  9. Phaa, phab and phac were amplifed from the subclone of pseudomanas sp. producing pha by pcr. the gel electrophoresis analysis showed that the molecular weights of cloned phaa, phab and phac were equal to fragment speculated from three orfs

    利用所提取的開放閱讀框架的序列設計三對引物，採用pcr技術，從合成pha的亞克隆片段中分離出phaa 、 phab和phac三個基因片段，經凝膠電泳分析表明，所克隆的三個基因分子量大小與推測的三個開放閱讀框架中基因片段大小一致。
  10. This unusual modification causes wild type s. lividans 1326 dna sensitive to site - specific oxidative double - strand cleavage ( dnd phenotype ). preliminary study revealed that such dna modification involves incorporation of sulphur. the entire functional dnd cluster, 8. 3kb in size, including 3 orfs - dnda, dndb and dndc was involved in this dna modification

    野生型變鉛青鏈黴菌具有一種不同於甲基化修飾的新型dna硫修飾系統，使dna在電泳時易遭到氧化雙鏈切割而導致降解（ dnd ， dnadegradation ）表型（ zhouetal . ， 1988 ， 1994 ， 1999 ） 。
  11. Ii ) two fragments ( about 240bp and 130bp ) were amplified from human keratinocytes total rna by rt - pcr. the recombinant pm - hpabl and pm - hpabs plasmids were constructed by inserting 240bp and 130bp pcr products into pmd 18 - t vector, respectively. the recombinants were identified by restriction enzyme analysis and dna sequencing, iii ) two orfs ( > 100bp ) were found in the insert sequence of pm - hpabl

    應用smartpcr試劑盒和簡並引物從人皮膚角質形成細胞cdna中擴增到長分別為24obp和13obp左右的2種片段，將它們插入pmd18一t載體，用酶切法初步篩選陽性重組子pm . hrabl和pm一hrabs ，對陽性重組子進一步作測序鑒定。
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