pcr amplification 中文意思是什麼

pcr amplification 解釋
pcr擴增
  • pcr : PCR ＝polymerase chain reaction 【生物化學】聚合酶聯鎖反應〈此項技術可用以復制犯罪現場發現的DNA小樣，從而有助於破案〉。
  • amplification : n. 1. 擴大；擴充。2. 【電學】增幅，放大（率）。3. （聲明等的）補充材料。
  1. Power for the thermal cycler shall be provided with a manostat or a ups to avoid the influence on pcr amplification arose by fluctuation of voltage

    熱循環儀的電源應專用，並配備一個穩壓電源或ups ，以防止由於電壓的波動對擴增測定的影響。
  2. A double - strand cdna fragment of dh ( diapause hormone ) gene was obtained from kt - pcr amplification. the fragment was digested by two restriction enzyme nde 1 / ecor 1 and then was joined with pet - 30a ( + ) plasmid which was digested by nde 1 / ecor 1 too. then the outcome was transformed into escherichia coli bl21

    Pcr產物經nde / ecor雙酶切后，與同樣雙酶切的pet - 30a （ + ）質粒連接並轉化至大腸桿菌（ escherichiacoli ） bl21中，經檢測篩選，成功得到陽性克隆。
  3. Cluster analysis based on rapd showed that at 76 % similarity level, all tested isolated could be clusted into nine groups, i. e. 1 p. ostreatus and p. florida, ii p. ostreatm p. sapidus, p. spodoleucus and p. eryngii, iii p. colnmbinus, p. corlicalus, p. cornucopiae, p. nebrodensis and p. ferulae ; iv p. pulmonarius and p. sajor - caju, v three isolates with indefinite species, vi p. luber - regium, vii p. cilrinopileatus, viii p. djamor and p. salmoneoslramincus, ix p. abalonus and p. cysliodism. 4. a single uniform product 1. 46kb in size resulted from pcr amplification of the 5 " half of the 28s rrna gene for all isolates of pleurolus and the other three genera

    隨機擴增多態性dna的聚類分析表明，在76相似水平下，可將供試的側耳菌株聚成九大類，第一大類包括糙皮側耳、佛羅里達側耳；第二大類包括美味側耳、灰白側耳、剌芹側耳；第三大類包括哥倫比亞側耳、裂皮側耳、黃白側耳、阿魏蘑、白阿魏蘑；第四大類包括肺形側耳和鳳尾菇；第五大類為3株未定名的側耳；第六大類僅有具核側耳；第七大類為金頂側耳；第八大類包括紅平菇和桃紅側耳；第九大類包括鮑魚菇和囊蓋側耳。
  4. With the improved method of ctab, the genomic dna extracted form plum and other species close to p. mume in genetic relationship could be directly used in pcr amplification, with good results obtained

    選用改良ctab法提取的梅及其近緣種的基因組dna可直接用於pcr擴增分析，且效果良好。
  5. The materials as explant in transformation come from birch leaf, stem segment and leaf stalk, and the spider toxin gene was used as foreign gene for this transformation experiment. it showed that the best explant was the big leaf, on which the transformation frequency was 22 %. by gus detection, there were 43 percent of the plants with kanamycin resistance, and 100 percent of positive result, by pcr amplification, was gotten from random sampling

    利用雙元載體的根癌農桿菌lba4404菌株（ agrobacteriumtumefaciens ） ，含質粒pyhy （目的基因及npt 、 gus基因） ，對白樺試管苗莖段，葉柄，葉片三種外植體進行侵染，結果表明：大葉片生長勢強，為轉基因的最優外植體，轉化率能夠達到22 。
  6. The probe detection and pcr amplification are contemporary, without ethidium bromide staining and gel running and any post - pcr manipulations, so that the contamination risks are reduced. those two methods were used to detect successfully the primus necrotic ringspot virus from sample delivered by beijing airport quarantine bureau. the amplified fragment was cloned into the vector of pmd18 - t then sequenced

    我們利用建立的雜交誘捕們pcr elisa和隊f pcr對北京機場檢疫局送檢的櫻桃樣品檢出了pnrsv ，同時把451hp的pcr產物克隆到ppo18 t載體上，通過序列測定，同源世最高的是genebank中pnrsv資料序號gi15750502gb山57046
  7. Dig labeled probe hybridization with solid pcr product was performed as well as electrophoresis of liquid product, this method combines rna purification, pcr amplification and nucleotide probe hybridization detection together and has many advantages including better rna purity, less time consumption, reliable positive reaction and 10 times sensitivity as rt - pcr gel running detection, reduce false positive result, unpurified nucleotide requirement, loug infected plant organism can be detected by solid hybridization

    2 ）結果可靠，雜交特異性誘捕目的片段，同時去除了核酸中存在的pcr抑制物質，減少了因核酸提取不純造成的漏檢現象，結果輸出通過雙重判定保證了結果的可靠性。 3 ）靈敏度高，通過雜交進行結果判定，靈敏度比傳統的rt干cr大約高10倍。
  8. Pcr amplification using 2 degenerate primers for nitrogenase fe protein gene was performed with chromosomal dna isolated from the 29 isolates. the result suggested that a nifh amplicon of 323 nucleotides was detected in 7 isolates and the 7 isolates are c4 c5 g1 g2, w5 t1 and t7. these pcr amplified fragments were cloned, and sequenced

    首先利用芽孢桿菌中芽孢的抗熱性將土壤溶液在100沸水中煮10 - 15分鐘，然後用選擇性無氮培養基進行初篩得到29株菌落形態不同的菌株；接著用固氮酶結構基因nifh的特異性引物對這29株菌進行pcr擴增，結果表明其中7個菌株具有nifh基因，這7個菌株的編號依次為： c4 、 c5 、 g1 、 g2 、 w5 、 t1和t7 。
  9. Plant defensin afp ( 238bp ) was amplified with six long complementary primers after two rounds of pcr amplification and plant expression vector peafp was constructed. 3

    通過合成六條長鏈引物，經兩次pcr擴增，獲得了238bp的植物防禦素afp基因全長，並構建了植物表達載體peafp 。
  10. Pcr amplification of the target sequence design a pair of primers ac - coding to the conservative region of hla - dqa1 exon 2, and lable the sence strand by fitc. make the fiuorescenced strand the advantage strand by assym - metric pcr. 3

    樣本靶序列的pcr擴增在hla - dqa1第二外顯子保守區域設計一對引物，並在正義鏈引物5 』作fitc標記，以熒光標記單鏈為優勢鏈，行不對稱擴增。
  11. Further we cloned full - length cdna of the ked like protein coding region via pcr amplification and confirmed its interaction with acam2 in yeast two - hybrid system

    進一步通過rt . pcr克隆了ked樣蛋白編碼區全長cdna ，通過雙雜交驗證了全長ked樣蛋白與acamz的相互作用，結果為陽性。
  12. Extraction of large - fragment genomic dna in order to gain dna template of pcr amplification ( long pcr amplification and salvage pcr amplification ) which was high purity and large fragment, three methods were used to extract genomic dna of bacillus subtilis, i. e. low melting - point agarose embedding method, sds - proteinase k - phenol chloroform extraction method and bacterial genomic dna extraction kit method. the genomic dna of bacillus subtilis were gained by these methods, and the operated programs of the methods were improved. the results showed that the genomic dna extracted by low melting - point agarose embedding method were obviously biggest than that of another two methods

    大片段基因組dna的提取為了獲得用於pcr擴增（長距離pcr擴增和分段pcr擴增）的高純度、大片段（至少為pcr產物長度的4倍）的dna模板，應用三種方法：低熔點瓊脂糖包埋法， sds -蛋白酶k -酚氯仿抽提法和細菌基因組dna提取試劑盒法，分別提取獲得了枯草桿菌基因組dna ，並對3種方法的操作程序進行了不同程度的改進，結果表明：低熔點瓊脂糖包埋法提取的基因組dna片段明顯大於后兩種方法，採用0 . 5瓊脂糖凝膠電泳3h ，仍然跑不出加樣孔。
  13. It also was a cosmopolitan species in the northern hemisphere. the phylogenetics relationships between the isolates of a. gallica from europe, north america and china were studied with issr - pcr amplification

    並對這5個蜜環菌生物種的地理分佈、生長規律、寄主范圍、培養特性等生物學習性進行了研究討論。
  14. They were capable of detecting 50 pg of purified total rna by a colorimetric assay and 5 pg by a chemiluminescent assay. the nonradioactive probe produced with pcr amplification were particularly suitable for practical diagnosis, as they are sensitive and can be rapidly prepared in large quantities. the two assay methods were 260 times and 26 times as sensitvity as r - page respectively

    利用含pstvd單體的pgem - 7z質粒，通過pcr擴增技術，用生物素標記制備cdna探針，進行雜交反應檢測pstvd ，其中通過化學顏色反應進行判讀靈敏度可達到50pg ，而用化學發光反應進行判讀靈敏度可達到5pg ，分別是r - page檢測靈敏度的260倍和26倍。
  15. The room for the following equipments need the air - conditioning : uv - spectrophotometer, fluorescence spectrometer, gc, gc - ms, aas, afs, icp, icp - ms, hplc, hplc - ms, ion chromatography, atomic fluores - cence spectrometer, pcr amplification and balance

    12紫外/可見分光光度儀、熒光分光光度，氣相色譜，氣相色譜質譜、原子吸收，原子熒光儀、等離子發射光譜、等離子質譜、高效液相、液相色譜質譜、離子色譜、熒光光度、 pcr核酸擴增儀以及天平室安裝空調。
  16. Benzyl chloride were used for extracting genomic dna of aspergillus. niger 14, about 1. 5kb specific fragment was obtained from genomic dna of aspergillus. niger 14 by pcr amplification with primers ( forward primer5 " ataggcatcatgggcgtctct3 " reverse - primer5 " cagctaagcaaaacactccgc 3 designed according to the known sequences of the phytase gene in the gene bank and pyrobest ? dna polymerase, after ligated with pmd18 - tvector, transformated into e. colidh5a competent cell successfully. 3. nucleotide sequence analysis of the cloned fragment revealed the presence of the whole phya gene in pcr product

    用氯化芐法提取了aspergillus . niger14 ~ #基因組dna ，根據genebank中已知的黑麴黴植酸酶基因序列設計出一對特異性引物（上游引物： 5 ataggcatcatgggcgtctc3下游引物： 5 cagctaagcaaaacactccgc3 ） ，採用pyrobest ~ （ tm ） dnapolymerase （高保真dna聚合酶） ，通過pcr方法從aspergillus . niger14 ~ #基因組dna中擴增出了預期的1 . 5kb左右的特異性產物，將其與pmd18 - tvector連接后，轉化e . colidh5菌株的感受態細胞，經質粒抽提、酶切鑒定，確認該目的產物已得到成功克隆。
  17. The gpa1 gene was obtained via pcr amplification and was cloned in the two - hybrid dna binding domain vector pgbkt7 the combinant plasmid was designated as pgbkt7 - ga. - galactosidase assay indicated that got did not have the property of self - activation. after pgbkt7 - ga was transformed to yeast pj69 - 4a, we transformed arabidopsis vegetative tissue two - hybrid cdna library plasmids to yeast pj69 - 4a containing pgbkt7 - ga

    通過pcr擴增得到gpa1基因並將其克隆到雙雜交dna結合域載體pgbkt7中，得到的重組質粒命名為pgbkt7 - g ， ?半乳糖苷酶活性鑒定表明g不具有自激活特性，將其轉化到酵母菌pj69 - 4a中，再以此為受體菌轉化擬南芥綠色營養組織cdna文庫質粒。
  18. The length of the 2nd intron sequences obtained by pcr amplification ranges from 153 to 168 bp in the 11 sisorid species ( 17 samples ) investigated, and is 154 bp in liobagrus kingi and liobagrus marginatoides

    通過pcr技術，擴增了長度為154bp的金氏（魚央）和擬緣（魚央）以及長度為153 168bp的11種?科魚類（ 17個樣品）的rps7基因內含子2序列。
  19. Liobagrus kingi and liobagrus marginatoides were designated as outgroups in the present study. the length of 16s rrna gene fragment obtained by pcr amplification ranges from 534 to 542 bp in the 11 sisorid species ( 31 samples ) investigated, and is 532 bp in l

    通過pcr技術，擴增了長度為532bp的金氏（魚央）和擬緣（魚央）以及長度為534 - 542bp的11種?科魚類（ 31個樣品）的16srrna基因片段。
  20. We cloned full - length cdna of ca coding region via pcr amplification, but a negative result was obtained when the interaction of full - length ca and ga was tested in yeast two - hybrid system

    通過rt - pcr克隆了ca編碼區全長cdna ，但是在雙雜交系統檢測全長ca與g蛋白的相互作用時，結果為陰性。
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