pcr (polymerase chain reaction) 中文意思是什麼

pcr (polymerase chain reaction) 解釋
基因擴增儀
  • pcr : PCR ＝polymerase chain reaction 【生物化學】聚合酶聯鎖反應〈此項技術可用以復制犯罪現場發現的DNA小樣，從而有助於破案〉。
  • polymerase : n. 【生物化學】聚合酶。
  • chain : n 1 鏈子，鏈條；項圈；表鏈。2 連鎖；連續，一系列，一連串；（山）脈。3 〈常 pl 〉鐐銬；羈絆，拘束...
  • reaction : n 1 反作用，反應；反沖；反動力。2 【政治學】反動，倒退；復古（運動）。3 【化學】反應，【物理學】...
  1. In this study, iltv - nm98a strain and iltv - wanggang strain were multiplied in chorioallantois. a pair of primers were devised according to the nucleic acid sequence of iltv tk gene and the dna of multiplied virus was used as pattern to amplify the gene of tk by polymerase chain reaction ( pcr ). the product of pcr was linked with suitable plasmid. then, the recombined plasmid was converted to escherichia coli. the converted escherichia coli

    根據已發表的iltvtk基因的核苷酸序列設計一對pcr引物，以增殖的兩株iltv的dna為模板，分別對它們的tk基因進行pcr擴增。將回收的pcr產物連接到適當的質粒載體上，轉化感受態大腸桿菌，通過篩選對iltvtk基因的陽性克隆進行擴增培養。
  2. Methods dna sequences of the coagulase were examined by polymerase chain reaction - sigle strand conformation polymorphism ( pcr - sscp ) method and were identified by sequencing

    方法利用酶切及sscp技術檢測凝固酶基因序列改變情況，並測序進行確證。
  3. In this study five recombinant plasmids containing hn ( hemagglutinin - neuraminidase ) genes of ndv ( newcastle disease virus ) were constructed, hn genes were amplified by reverse transcriptase - polymerase chain reaction ( rt - pcr ) and were inserted directly into eukaryotic expression plasmid pcdna3

    應用反轉錄-聚合酶鏈反應（ rt - pcr ）獲得兩株新城疫病毒（ ndv ）血凝素-神經氨酸酶（ hn ）基因cdna ，然後定向插入真核表達質粒pcdna3中，構建了含hn基因的重組質粒。
  4. A pair of primers were chemically synthesized based on the cdna sequence of hog cholera virus strain brescia and used to amplify the cdna fragments of envelope glycoprotein e2 gene of hclv which coding the major protective antigen by reverse transcription - polymerase chain reaction ( rt - pcr ) from total intact rna extracted from infected calf testicle cell culture by hclv

    以豬瘟兔化弱毒犢牛睪丸細胞毒（ hclv ）中提取的細胞總rna為模板，以rt - pcr技術，擴增出了完整e2基因的cdna片段。經電泳、酶切分析，證實了所擴增片段為e2基因特異性片段。
  5. The nucleoprotein ( n ) gene of three strains and glycoprotein ( g ) gene of 119, gxbm were amplified by reverse transcription - polymerase chain reaction ( rt - pcr ), respectively

    對三株的n基因和119 、 gxbm株的g基因進行了rt - pcr擴增、克隆和測序。
  6. Pcr method for detection of salmonella with polymerase chain reaction

    用聚合酶鏈反應
  7. Marek " s disease virus ( mdv ) phosphoprotein 24 ( pp24 ) gene was amplified from md11 strain by polymerase chain reaction ( pcr ). then we cloned it into the downstream of gst gene according to the right open reading frame ( orf ) in pgex - 6p - l vector

    本研究將型mdvmd11株的pp24基因的完整orf克隆入原核表達載體pgex - 6p - 1中，重組質粒pgex - pp24轉化bl21宿主菌后，經iptg誘導表達。
  8. In this paper, 45 e. coli strains isolated from chicken farms in sichuan province were determined to be the pathogenic e. coli by animal test. type 1 pili of 45 strains isolated was detected by msha. the pila gene of 45 avian pathogenic e. coli strains were amplified by the polymerase chain reaction ( pcr ) with primers designed according to the sequence of the pila gene in genbank. results showed that pcr was more sensitive, faster and more characteristic than msha to detect type 1 pili

    本研究將從四川規模化雞場分離鑒定、經1日齡雛雞致病性試驗得到的雞源致病性大腸桿菌45株，採用d -甘露糖敏感血凝試驗（ msha ）檢測1型菌毛，根據genbank中公布的人源大腸桿菌1型菌毛pila基因序列設計一對引物用pcr擴增雞源致病性大腸桿菌1型菌毛pila基因。
  9. Polymerase chain reaction - restriction fragment length polymorphism, pcr - rflp

    限制性片段長度多態性
  10. To study on structure and inheritance of rh d gene interaction between gene expression of rh d and rh c / e and influences on rh d gene expression of inserts and rh box - methods : 20 pairs of oligonucleotide specific primers for exon, intron 2 4, insert and rh box of rh d, rhc / e gene were designed and composed the polymerase chain reaction - sequence specific oligonucleotide primer ( pcr - ssp ) was used to amplify the rh c / e gene, rh d gene, exon, intron, insert and rh box in 106 samples of unrelated individuals and 7 han nationality ancestries and 5 wei nationality ancestries whose proband were rh d - negative

    目的：觀察中國漢族非血緣關系隨機個體、漢族及維吾爾族家系rh血型的c e基因、 d基因外顯子、內含子、插入片段以及rhbox ，研究rhd基因結構及遺傳規律， d基因表達與c e基因的關聯，以及插入片段和rhbox對d基因表達的影響。
  11. In this work, some species of palmae cultivated in the xiamen botanical garden had been selected to be analyzed their genetic diversity with rapd ( random amplified polymorphic dna ) technique. according to the result of genomic dna amplified with pcr ( polymerase chain reaction ), genetic distance and similarity between different palm species were calculated on nei " s estimate of similarity and genetic distance. a primary but first time research at the phylogenetic relationships of some genera and species, the molecular classification and identification of some puzzling species of palmae was carried out through upgma ( unweighted pair group mean average ) cluster analysis of the genetic distance together with comparative study of the morphological structure characteristics

    本文在初步調查分析了棕櫚科植物在我國的自然分佈、引種馴化情況以及該類植物在廈門地區栽培應用狀況的基礎上，首次採用rapd分子標記技術，對廈門萬石植物園引種的一些棕櫚科植物的遺傳多樣性進行了研究，根據pcr對基因組dna擴增的結果，用nei ' s相似性系數計算了不同植物間的遺傳距離和遺傳一致度，通過對遺傳距離的upgma聚類分析，並結合形態分類的特點，對棕櫚科植物的屬、種間的系統分類關系和一些疑難種的分類鑒定進行了初步研究。
  12. We utilized microarray and real - time quantitatie polymerase chain reaction ( pcr ) to identify genes differentially expressed at 6 h reperfusion in periinfarct cortex from castrated rats with or without dht replacement

    我們利用微點陣和實時定量pcr方法在再灌注6小時時，對進行/未進行dht替代的閹割大鼠梗死周圍皮層的基因差異表達進行了鑒定。
  13. We utilized microarray and real - time quantitative polymerase chain reaction ( pcr ) to identify genes differentially expressed at 6 h reperfusion in periinfarct cortex from castrated rats with or without dht replacement

    我們利用微點陣和實時定量pcr方法在再灌注6小時時，對進行/未進行dht替代的閹割大鼠梗死周圍皮層的基因差異表達進行了鑒定。
  14. The percentage of polymorphic sites, degree of genetic polymorphism and genetic distance were compared and the phylogenetic tree was constructed by neighbor - joining method. the partial mitochondrial 16s rrna gene was amplified by polymerase chain reaction ( pcr ) and the pcr products were directly sequenced after purified. these sequences, together with the homologous sequences of another trichiuridae species lepidopus caudatus obtained from genbank were used to analyze nucleotide difference and to establish a upgma phylogenetic tree by means of biological informatics

    汝us價ay1830 ）各12個個體進行rapd分析，對比多態位點比例、遺傳多態度以及遺傳距離，並構建neighbor - join噸系統樹;通過pcr擴增出線粒體165rrna基因，純化后直接測序，利用生物信息學方法進行序列分析和核昔酸變異比較，結合ge紅bar止中大西洋叉尾帶魚（ lepid （護腳caud玫tuseuphrasen1788 ）同源序列構建u甲cm叭系統樹。
  15. By using single strand conformation polymorphism analysis of polymerase chain reaction ( pcr - sscp ), the frequency of codon 54 mutant allele of mbl structural gene in the population of hans was investigated. 3. the molecular beacon ( mb ) hybridization technique with visual monitoring was established

    建立聚合酶鏈反應單鏈構象多態性分析inglestrandconforma tlonpolymorphlsmanalysisofpol仰erasechainreactionproduction ， pcr sscp ）方法，初步調查漢族人群mbl基因ggc54gac突變情況。
  16. Protocol of reverse transcription polymerase chain reaction rt - pcr for infectious haematopoietic necrosis virus

    傳染性造血器官壞死病毒逆轉錄聚合酶鏈式反應操作規程
  17. Polymerase chain reaction, pcr

    方法採用熱啟動聚合酶鏈反應
  18. Founder mice will be examined by polymerase chain reaction ( pcr ) and southern blot

    採用聚合酶鏈式反應（ pcr ）和southern印跡對陽性鼠進行檢測。
  19. Pcr ( polymerase chain reaction ), southern blot analysis performed toward 10 transgenic rice plants revealed that foreign genes were integrated into the genome of these plants

    選取部分植株進行pcr分析、 dna點雜交、 pcr southern雜交。以上分子檢測結果表明，絕大部分經抗性篩選得到的轉化植株的基因組中已整合有外源基因。
  20. Pcr polymerase chain reaction

    聚合酶鏈反應
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