polymerase 中文意思是什麼

polymerase 解釋
n. 名詞 【生物化學】聚合酶。

  1. Comprehensive cellular responses was found in human amnion fl cells following exposure to low concentration of mnng, such as the lowering of dna replication fidelity resulted from alteration of dna polymerase profile ; activation of a lot of transcription factors, such as api, creb, nf - kb etc ; clustering of egfr ( epidermal growth factor receptor ) and tnfr ( tumor necrosis factor receptor ) and activation of camp - pka - creb and jnk / sapk signal pathways

    我們發現，低劑量mnng處理后的人羊膜fl細胞有廣泛的細胞反應，並有多個信號轉導通路的激活和基因表達的改變。例如dna復制保真度下降， dna聚合酶譜發生改變，應用報告基因技術和底物磷酸化檢出技術證明細胞一系列轉錄因子如ap1 、 creb 、 nf b等被激活，細胞表面受體如表皮生長因子受體、腫瘤壞死因子受體發生聚簇，細胞信號轉導通路camp - pka - creb和jnk sapk被激活。
  2. In our experiment, the specific fragment was amplified from transgenic bobwhite genome dna at annealing temperature 61 by using high - fidelity pfu dna polymerase and cloned into clone vector pgem - 7fz ( + ), then sequenced. the cloned sequence was completely identical to the sequence which was issued in genbank

    本實驗採用了高保真pfudna聚合酶，在退火溫度61條件下從轉基因bobwhite品種基因組dna中擴增出特異性片段，將此片段插入克隆載體pgem - 7fz （ + ） ，經測序和序列分析表明，所擴增得到的片段含有bar基因完整的讀碼框，並且序列與genbank中發表的序列完全一致。
  3. The insulin - like growth factor - 2 ( igf - 2 ) and igf conjugated protein - 6 ( igfbp - 6 ) mrna level in rat calvaria bone tissue and mc - 3t3 - el cells were detected by northern blotting analyses and reverse transcription polymerase chain reaction. the estrogen responsive element ( ere ) in igfbp - 6 gene promoter was identified and involved in tcdd - reduced regulation of the gene expression by electromobility shift assays ( emsa )

    正常胎鼠頭蓋骨組織igf一2mrna呈高水平表達狀態，而igfbp一6mrna的水平較低; ccf胎鼠頭蓋骨骨組織內igf一2mrna的表達較正常胎鼠降低， igfbp一6mrna的表達則明顯升高; atra和e :聯合應用時， atra可以抑制雌激素對細胞內igf一2和igfbp一6的這種調節作用。
  4. In this study, iltv - nm98a strain and iltv - wanggang strain were multiplied in chorioallantois. a pair of primers were devised according to the nucleic acid sequence of iltv tk gene and the dna of multiplied virus was used as pattern to amplify the gene of tk by polymerase chain reaction ( pcr ). the product of pcr was linked with suitable plasmid. then, the recombined plasmid was converted to escherichia coli. the converted escherichia coli

    根據已發表的iltvtk基因的核苷酸序列設計一對pcr引物，以增殖的兩株iltv的dna為模板，分別對它們的tk基因進行pcr擴增。將回收的pcr產物連接到適當的質粒載體上，轉化感受態大腸桿菌，通過篩選對iltvtk基因的陽性克隆進行擴增培養。
  5. Methods dna sequences of the coagulase were examined by polymerase chain reaction - sigle strand conformation polymorphism ( pcr - sscp ) method and were identified by sequencing

    方法利用酶切及sscp技術檢測凝固酶基因序列改變情況，並測序進行確證。
  6. Beta - exotoxin is an inhibitor of rna polymerase and acts competitively with atp in various biological processes

    外毒素是rna聚合酶的抑制劑，在各個生化過程中與atp起競爭作用。
  7. Detection of h. pylori in gastric mucosa, gastric juice and saliva in children with polymerase chain reaction

    技術在兒童幽門螺桿菌感染中的應用
  8. Even minute amounts of dna can now be amplified, using the polymerase chain reaction, to provide sufficient material for genetic fingerprinting

    即使僅獲得很少量的dna也可使用聚合酶鏈式反應來擴增以提供足夠的材料進行遺傳指紋分析。
  9. In this study five recombinant plasmids containing hn ( hemagglutinin - neuraminidase ) genes of ndv ( newcastle disease virus ) were constructed, hn genes were amplified by reverse transcriptase - polymerase chain reaction ( rt - pcr ) and were inserted directly into eukaryotic expression plasmid pcdna3

    應用反轉錄-聚合酶鏈反應（ rt - pcr ）獲得兩株新城疫病毒（ ndv ）血凝素-神經氨酸酶（ hn ）基因cdna ，然後定向插入真核表達質粒pcdna3中，構建了含hn基因的重組質粒。
  10. A pair of primers were chemically synthesized based on the cdna sequence of hog cholera virus strain brescia and used to amplify the cdna fragments of envelope glycoprotein e2 gene of hclv which coding the major protective antigen by reverse transcription - polymerase chain reaction ( rt - pcr ) from total intact rna extracted from infected calf testicle cell culture by hclv

    以豬瘟兔化弱毒犢牛睪丸細胞毒（ hclv ）中提取的細胞總rna為模板，以rt - pcr技術，擴增出了完整e2基因的cdna片段。經電泳、酶切分析，證實了所擴增片段為e2基因特異性片段。
  11. Dr kornberg worked out the details by crystallising the complex of dna, rna and polymerase at various stages of the process

    考恩伯格博士通過對此過程中各個階段的dna 、 rna和聚合酶的復合體進行結晶，研究出這一過程的詳細情況。
  12. As bases are added by polymerase to the starting point of a new complementary strand, known as a primer, or recognized by ligase as a match, the template ' s sequence is revealed

    當聚合酶將一個核苷酸加在新互補鏈的起始引子之後，或接合酶認定某段核苷酸鏈與原始模版配對，就可利用這些反應來得出原始模版的序列。
  13. Eukaryotic messenger rnas are synthesized by the multisubunit enzyme rna polymerase ii, aided by myriad cofactors that control different events in this multistep process

    其mrna的合成是個多步驟的復雜過程，由控制該過程的多個輔因子協助rnap完成。
  14. The results of these early research work showed that rna polymerase transcription was localized in the nucleoli and rna polymerase and in the nucleoplasm

    當時的研究結果顯示： rna聚合酶的轉錄發生在核仁內， rna聚合酶和rna聚合酶的位於核質中。
  15. In recent years, more and more scientists presumed that rna polymerase transcription might not occur in the nucleoplasm but in the nucleoli. nevertheless, the possibility has not been proved directly

    近幾年，有許多學者相繼提出了rna聚合酶有可能在核仁區域內發生轉錄的觀點，但是這一觀點至今還沒有得到直接的實驗證據的支持。
  16. It is inferred that its active transcription occurs in the same region, not the nucleoplasm. the result will help us to further comprehend the mechanism of rna polymerase transcription, the way of its transcripts processing and transport, and the structural and functional relationship among the three rna polymeraes

    這一結果不僅直觀地向人們表明了rna聚合酶在真核細胞核中的轉錄位點，而且對於人們進一步認識和理解rna聚合酶的轉錄機制、其轉錄產物的加工運輸途徑、以及真核細胞當中不同的rna聚合酶間的組織和調控關系都將有著重要的理論意義。
  17. From these results, it is inferred that the active transcription of pol iii occurs in the nucleoli and its periphery region, but not the nucleoplasm. the result will help us to further comprehend the mechanism of rna polymerase iii transcription, the way of its transcripts processing and transport, and the structural and functional relationship among the three rnapolymeraes

    本實驗為rna聚合酶在真核生物細胞核中的轉錄位點提供了較為直接的證據，這對人們進一步了解rna聚合酶的轉錄機制、加工和運輸過程及三種rna聚合酶之間的結構與功能關系具有重要的意義。
  18. The results of these early research work showed that rna polymerase iii transcription was localized in the nucleoplasm. however, with the development and the application of new technologies since 1990s, the controversy arose on the transcription sites of rna polymerase iii. in recent years, more and more scientists presumed that rna polymerase iii transcription might not occur in the nucleoplasm but in the nucleoli

    自上個世紀八十年代初期，人們相繼運用細胞化學染色、電鏡放射自顯影等進行研究的結果表明： rna聚合酶的轉錄發生在核質中，但隨著新的研究技術的發展和應用，人們卻發現rna聚合酶的轉錄可能發生在核仁中，從而對早期的研究結果提出了質疑。
  19. The nucleoprotein ( n ) gene of three strains and glycoprotein ( g ) gene of 119, gxbm were amplified by reverse transcription - polymerase chain reaction ( rt - pcr ), respectively

    對三株的n基因和119 、 gxbm株的g基因進行了rt - pcr擴增、克隆和測序。
  20. 2. 5 ul of 10 x reaction buffer, 1. 5 ul 25mm mgcl2, 0. 3 ul lomm dntp, 0. 5 ul taqdna polymerase ( 5 u / ul ), and lul ( = 20ng ) of primer were used in per reaction. each reaction was overlaid with one drop of paraffin oil. the initial denaturation step was used at 94 for 1 min 45 sec ; then denatured at 94 for 30 sec, annealed at 37 1 min, extended at iv b 72 for 2 min and repeated the cycle 45 times, at last, extended at 72 ' c for lomin

    等（ 1995 ）利用rapd標記區分美國東部一雜交地帶的蟋蟀的兩個姐妹種， allonemobiusfasciatus和a . socius ，並於1998年使用rapd和異型酶標ic做出了這兩種蟋蟀的基因連鎖圖；國內田英芳、鄭哲民（ 20of ）首次將rapd技術運用於蟋蟀總科的分子系統學研究中，採用2種引物對7種蟋蟀進行了基困組dna多態性研究，並應用upg問a法構建樹狀圖，椎測系統發生關系
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