pepstatin 中文意思是什麼

pepstatin 解釋
胃（蛋白）酶抑制劑，抑胃酶肽
  1. The he was strongly inhibited by sbti and apmsf, and very sensitive to pmsf, lbti, and tlck, while not sensitive to chymostatin, bestatin, leupeptin, tpck, pepstatin, nem and iam. all these results imply that brine shrimp he was most probably a trypsin - type serine protease. the he could be strongly inhibited by edta in a dose - dependent manner, and 50 mmol / l edta exhibited more than 56. 5 % inhibition

    對孵化酶純化樣品進行生化性質和酶性質分析發現，鹵蟲孵化酶的最適反應溫度約為40 ，最適ph為8 . 5左右；該孵化酶對p - apmsf 、 sbti極為敏感，對pmsf 、 lbti和tlck也非常敏感，但對chymostatin 、 leupeptin 、 pepstatin 、 bestatin 、 tpck 、 nem和iam不敏感，表明該酶極可能是一種屬于胰蛋白酶類型的絲氨酸蛋白酶。
  2. Camp, pka inhibitor peptide ( pki ), kemptide, histone h1 ( type iii - s ), polyvinyl alcohol, na3vo4, naf, b - glycerophosphate, p - nitrophenylphosphate, 3 - [ n - morpholino ] - propanesulfonic acid ( mops ), genistein, ml - 9, leupeptin, aprotonin, pepstatin, chymostatin, trypsin - chymotrypsin inhibitor were purchased from sigma chemical co. [ r - 32 p ] atp was obtained from yahui biomedical enginnering co

    實驗材料昆明系小白鼠，中國醫科大學實驗動物部提供。測活用試劑除nacl山inni為國產分析純對rr為amesa產品， y乍jatp為北京亞輝生物醫學工程公司產品外，余者均為sipoa產品。
  3. The enzyme activity could be completely inhibited by pmsf and dfp, and partially inhibited by pepstatin a, edta and egta. leupeptin, benzamidine and hydrochloride aprotin had no influence on the dhap activity. ca2 *, mg2 + and na * could slightly increase, but cu2 + and zn2 + slightly inhibited protease activity

    此結果表明， dhap為一種絲氨酸蛋白酶，其酶活性的發揮需要某些金屬離子的參與，如na + ， c擴+ ， m擴+等金屬離子能提高dhap的活性，但c了+與z矛十能抑制dhap的部分活性。
  4. Western blot analysis we treated 200 fertilized eggs in a series of steps including lysing them in l0ul of lysis buffer ( 50mm tris - hcl ph 7. 5, 250mm nacl, 5mm edta, imm dtt, 0. 1 % triton, 50mm sodium orthovanadate, looug / mg pmsf and tpck, 50ug / ml tlck, 1 ug / ml leupeptin pepstatin and aprotinin ), frozing them below - 70, and then thawing them at room temperature for 10 min

    2 . westem印跡將處理組及對照組小鼠一細胞g2期受精卵各200個取出，轉移到ep - pendoff管中， 5000甲m離心4分鐘，棄去上清，加入ro川粉碎緩沖液，充分振蕩混勻，在液氮中經過2一3次的凍融循環，迫使卵細胞裂解，加人等量的樣品緩沖液沸水中煮5分鐘，用於westem印跡分析。
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