phagemid 中文意思是什麼
phagemid
解釋
噬粒-
Through primary pcr. and finally the recombinant dna fragments were cloned into the phagemid pcantab 5e vector and introduced into
十五肽的接頭連在一起，並克隆到噬菌質粒pcantab 5e中，電擊轉化大腸桿菌tg1 。 -
The phagemid particles displaying functional scfvs were rescued by reinfection of helper phage m13ko7, thus a murine antibody library was obtained
經輔助噬菌體m13ko7超感染回收全部重組噬菌體，此即噬菌體抗體庫。 -
A polypeptide with sequence of qkvdssggggs was designed to be a linker between c terminal of penicillin g acylase and n terminal of the coat protein. the ribosome binding site ( rbs sequence ) of psurfscript is also replaced by rbs sequence originating from bacillus subtilis. it was demonstrated that constructed phagemid can still express penicillin g acylase
將包含信號肽和琥珀終止密碼子uag （ amber ）的完整巨大芽孢桿菌青霉素g酰化酶基因克隆到噬菌粒psurfscript ，通過引入的11肽連接青霉素g酰化酶的c末端與噬菌體外殼蛋白gp3的n末端。 -
It is firstly reported that the 85. 6kda heterodimeric penicillin g acylase from bacillus megaterium was processed and functional displayed on the surface of phage fd. phagemid psurfscript was used to clone full - lenghth gene with amber stop codon of penicillin g acylase ( signal peptide - subunit - interaal peptide - subunit ) from bacillus megaterium
革蘭氏陰性菌alcaligenesfaecalis的pga已在噬菌體表面獲得展示，並證實有活力，革蘭氏陽性菌來源的pga是否能在噬菌體表面成功展示，迄今尚未見報道。
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