plaque assay 中文意思是什麼

plaque assay 解釋
蝕斑測定
  • plaque : n. 1. （象牙、陶磁等制的）飾板；匾。2. （表示地位、名譽的）胸章；徽章。3. 【醫學】斑；（噬菌體）溶菌斑；血小板。
  • assay : n 1 化驗；分析；鑒定，測定，驗定。2 被分析物，被化驗物。3 化驗結果，化驗報告。4 〈古語〉企圖，嘗...
  1. Water quality - detection of human enteroviruses by monolayer plaque assay

    水質.用單分子層噬菌斑序列檢測人體腸病毒
  2. The homology of recombinant virus bmpak - hbmp was obtained and identified by plaque assay and baculovirus contains the hbmp gene was confirmed through pcr and dna dot blotting. the expressed rhbsag was determined by elisa after infecting bm - n cells and pupae with recombinant virus bmpak - hbmp and bmpak - hbm ( containing nonfusion hbv surface antigen medium sized )

    用重組病毒bmpak - hbmp和bmpak - hbm [帶有非融合乙肝表面抗原（ pres2 + s ）基因，為本實驗構建]感染家蠶細胞及蛹，對兩種病毒的表達產物用elisa進行了跟蹤檢測，結果表明，感染bmpak - hbmp的家蠶細胞及蛹中rhbsag的表達量分別為3
  3. The angiostatin baculovirus transfer vector was co - transfected with viral dna into sf9 cells according to the manufacturers protocol. to purify the recombinant virus, we used the plaque assay to screen the pure recombinant plaque and amplify it to generate p - 1 stock

    構建重組病毒：用已經構建好的angiostatin桿狀病毒轉移載體pbluebachiszb和病毒dna共同轉染sfg細胞，通過蝕斑實驗篩選出純的重組斑點並擴增產生p二病毒貯存液。
  4. The immunomodulatory effects of crude product were detected by lymphocyte transformation test ( lit ) and plaque forming cell assay ( pfc )

    Kp莢膜糖蛋白粗產品免疫功能檢測採用淋巴細胞轉化試驗（ mtt法）和溶血空斑試驗，分別測定細胞免疫和體液免疫功能。
  5. The recombinant meq - baculovirus was obtained by co - transfecting the insect sf9 cells with pblubac4 - meq and linearised bac - n - blue dna. the recombinant baculovirus was selected by plaque assay and confirmed by pcr technique and sequencing of the inserted gene

    應用重組痘病毒表達的meq制備的單抗23b46對重組桿狀病毒感染的sfg細胞及其裂解物分別進行間接免疫熒光試驗、 westemblot和免疫沉澱試驗的檢測。
  6. Firstly, a reverse hemolytic plaque assay ( rhpa ) was used to scale testosterone secretion of leydig ' s cells in single cells level. sequentially, star mrna expression was detected with fluorescent in situ hybridization ( fish ) at the same cells

    本研究首先採用逆向溶血斑法，鑒定單個間質細胞睪酮分泌上的差異，然後，在被鑒定的同一間質細胞上進行原位雜交（ fish ） ，以便在單個細胞水平上確定間質細胞是否存在功能及star基因表達的異質性。
  7. After obvious cytopathogenic effects developed, virus - contained supematants were harvested, and the progeny viruses were screened for lacz - expressing viruses by a plaque assay using x - gal. single blue plaques were picked, and a recombinant prv stably expressing lacz gene ( designated as rprv - lacz ) was obtained after ten cycles of plague purification and pcr identification. the results showed that the lacz gene expression cassette was stably expressed in the recombinant rprv - lacz derived from bartha - k61 strain

    該載體與具有高度感染性的bartha - k61株基因組dna通過脂質體加plus法共轉染vero細胞，採用甲基纖維素固定病變， x - gal染色，經過10代藍斑純化獲得了一株穩定表達lacz基因的ge tk基因缺失突變株，命名為rprv - lacz 。
  8. Finally, in order to investigate whether these two virus are resistant to complement attack, and whether the resistance were mediated by incorporating cellular complement regulators of cd55, cd59 into their out membrane, complement lysis assay and plaque reduction assay were carried out respectively to find the lysis or neutralization activity of complement to hiv and eev under the condition of with or without the cd55 and cd59 blocking antibody

    將這兩種病毒分別暴露於人血清補體后，發現在有cd55 、 cd59中和性抗體存在的情況下，補體溶解hiv病毒的效率明顯增加； eev病毒的感染活性在空斑實驗中也明顯降低，結果與這兩種蛋白在病毒表面（ eev ）存在的事實相符，提示病毒表面的cd55
  9. Water quality - detection of human enteroviruses by monolayer plaque assay ; german version en 14486 : 2005

    水質.通過單層噬菌斑檢驗探測人體腸道病毒
  10. In order to get the soluble recombinant eo protein and inspect the protein expression status convinently, the egfp and eo gene were ligated into baculovirus transfer vector. with the co - transfecting sf9 cells of baculovirus recombinant transfer vector and linearized viral dna, and plaque purification in the posttransfection procedure, the pure recombinant baculovirus were harvested, which infected the sf9 cells for amplifying to generate a p - l stock. in the meantime, the fluorescence microscopy detection indicated expressed egfp protein to confirm the heterogenous protein expression of recombinant baculovirus. the pi - stock from a pure plaque was used to generate a high liter p - 2 stock, which was determined in liter as 1. 14 107pfu / ml by performing a plaque assay. when a volume of p - 2 stock infected the sf9 cells with moi 5 - 10 for expression, the strong fluorescence was obeserved on the day 3 of postinfection

    此外，為了得到可溶性重組eo蛋白並便於觀察重組蛋白的表達情況，我們將egfp基因與eo基因相連插入昆蟲桿狀病毒轉移載體中，與線性桿狀病毒dna共轉染sf9細胞后通過噬斑純化得到純的重組桿狀病毒，將其感染sf9細胞制備p1種子液，同時用熒光顯微鏡觀察綠色熒光蛋白的表達情況剔除表達效果差的重組桿狀病毒。再用p1種子液感染sf9細胞制備高效價的p2種子液。通過病毒液的梯度稀釋和噬斑測定，確定p2種子液的病毒滴度達1 . 14 10 ~ 7pfu ml 。
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