plasmid antibiotic-resistance 中文意思是什麼

plasmid antibiotic-resistance 解釋
plasmid抗生素拮抗質粒
  • plasmid : 質粒；原核質體
  • antibiotic : adj. 1. 破壞[傷害]生命的。2. 抗生的，抗菌的。n. 抗生素。
  • resistance : n. 1. 抵抗，反抗，抗拒，抵禦；敵對，抵抗力，反抗力，阻力，【生物學】抗病性。2. 【電學】電阻；阻抗；電阻器。
  1. Kurstaki strain hd73, were inserted into two copy sets of res sites. the res sites have same direction. when - the recombinant plasmid was introduced into crystal negative b. thuringiensis host bmb171, antibiotic resistance genes and other non - 5, thuringiensis dna can be selectively eliminated after the selection by antibiotic resistance marker

    將crylac10基因或壯觀黴素基因和蘇雲金芽胞桿菌的質粒復制起始區oril030連接在一起，置於兩個同向的解離區之間，再將基因操作中所必需的大腸桿菌質粒復制起始區和抗生素標記基因等與之相連構成解離載體。
  2. The abuses of antibiotics in medicine and livestock exposed to environmental bacteria lead to a large - scale dissemination of antibiotic - resistance bacteria in aquatic environment under selective pressure and the resistant organism could transfer resistance genes across the genus and species by plasmid and integron, antibiotic resistance microbes are common in aquatic environment and the aquatic environment has become a major reservoir for antibiotic - resistant microbes

    摘要臨床和畜禽業抗生素的濫用導致微生物在選擇性壓力作用下獲得並維持耐藥性，並有可能通過質粒和整合子將耐藥基因在相同或不同種屬中廣泛傳播轉移，最終導致多重耐藥。
  3. A bt - e. coli shuttle vector pht315 was deleted its replication region of bt, then constructed a novel vector named pht315 - 1 which composed a multiple cloning site, erythromycin and ampicillin - resistance marker and could only replicated in e. coli. used pht315 - 1, a 5273 bps dna fragment carrying a novel bt plasmid replicon was isolated and registered in genbank as ay278324. sequence analysis showed that there were at least three orf ( open reading frame ) in the cloned dna encoding 501, 333, 183aas. orfl had 98 % identities with replicating related protein ori43 of bt strain hd263. the others were no homology to any published bt replicating related protein. after continuous cultured for 70h at 30 c without antibiotic selecting press. the stability of plasmid carrying cloned replicon in bt acrystalliferous mutant strain hd73 cry was more than 98 %. and growth curve also showed that the novel replicon was stable and could replicate normally

    進一步序列分析表明該復制區至少有3個較大的orf ，分別編碼501 ， 333 ， 183個氨基酸。其中orf1蛋白序列與hd263復制蛋白ori43的同源性為98 ，而另外兩個orf和genbank己公布的bt復制相關蛋白無同源性。 30連續培養72h ，復制區質粒在bt無晶體突變株hd73cry ~ -中穩定性達98以上， 30h生長曲線也表明該復制區能夠在bt中穩定復制和遺傳，對受體菌株無明顯不良影響。
  4. With high concentration of sds and elevated temprature （ 43 ） ， we succeeded in eliminating plasmids from ten strains of s. aureus which were isolated from the farms of beijing suburb. according to the sensitivity test before and after plasmid remoral ， we found that 72. 5 antibiotic resistance of s. aureus was mediated by plasmids ， and 27. 5 encoded by chromosome. the curing test indicated that the plasmids play an important role in coding the resistance to antibiotics

    從北京郊區雞場分離到10株耐藥性金黃色葡萄球菌，對這些菌株進行質粒消除，根據質粒消除前後的耐藥性檢測，發現耐各種藥物的抗性基因大多數位於質粒上，少部分抗性基因位於染色體上，由質粒介導的耐藥基因佔72 . 5 % ，由染色體編碼的抗性基因佔27 . 5 % ，說明質粒在決定對抗生素的抵抗性中起主要作用。
  5. The antibiotic resistance of this strain is detected, because it generally relates to the plasmid. the result shows that this strain has not antibiotic resistance to chloramphenicol and tetracycline, but ampicillin

    因為細菌的抗生素抗性往往是與質粒相關的，所以考察了這株菌對抗生素的抗性，結果表明，這株菌對氨芐青霉素有抗性，對氯黴素和四環素沒有抗性。
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