plasmid chimera 中文意思是什麼
plasmid chimera
解釋
質體鑲嵌-
Methods : the two pairs of designed primers were based on pzp3 a and hcg p - ctp109 - 145 cdna sequences. the pzp3 a - hcg p - ctp109 - 145 chimera was amplifiled by overlapping pcr. the chimera was cloned into ppic9k plasmid and transformed into e. coli dh5 a
結果: 3步pcr擴增出pzp3 - hcg - ctpdna片段,插入到載體質粒ppic9k的克隆位點,獲得重組ppic9k - pzp3 - hcg - ctp表達質粒,測序結果顯示插入序列與設計預期完全一致。
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