plasmid cloning vector 中文意思是什麼
plasmid cloning vector
解釋
質粒克隆載體-
The recombinant transfer vector pbacpak - hbmp was constructed by insertion of the hbmp coding sequences into the multiple cloning site of transfer vector pbacpak. 8. bmn cell line was co - transfected with pbacpak - hbmp plasmid and linearized baculovirus bacpak6 dna by dosper agent
將克隆到的hbmp基因通過適當的酶切插入到轉移載體質粒pbac - pak8的多克隆位點中，獲得重組轉移載體質粒pbacpak - hbmp 。 -
Since the plasmid is capable of independent replication in host cells of many dicotyledonous plants, it has been used as a cloning vector in gnetic engineering
在許多雙子葉植物中質粒在寄主細胞中是獨立的復制單元，所以可以在基因工程中用作克隆載體。 -
Streptomyces low copy number plasmid scp2 * is also employed for the cloning and recovery of large dna fragment from streptomyces. as scp2 * can accommodate large dna insertions and is efficiently transmissible among its permissive hosts such as s. lividansbut seemed to be unable to replicates in streptomyces sp. fr - 008, it might be a suitable vector for the cloning of the entire fr - 008 pks gene cluster through gene replacement followed by conjugation with 5
Scp2 *可以在變鉛青鏈黴菌等許可宿主內復制並在許可宿主間有效轉移，但似乎不能夠在鏈黴菌fr - 008中復制，因此可能適合於在鏈黴菌fr - 008中通過進行基因置換及隨后的鏈黴菌fr - 008和變鉛青鏈黴菌間的接合過程來克隆完整的fr - 008生物合成基因簇。 -
When compared with kds301 ( lexa + ), xe ( lexa - ) cells showed quite similar levels of survival curve to y - irradiation and mmc, indicating lexa gene had no effect on radiation resistance of deinococcus radiodurans. deinococcus radiodurans " lexa had no revelance with its extremely radioresistance. the recombinant plasmid pza172 was constructed by cloning the lexa gene of deinococcus radiodurans into the vector plasmid puc19 in the downstream of lacz promoter
對lexa基因突變體xe的研究表明，其輻射抗性與lexa基因野生型的抗輻射菌kd8301相近，存活曲線上均顯示有一個寬大的肩區，結果發現lexa基因的突變並沒有改變抗輻射菌的獨特抗性，說明lexa基因與輻射抗性無關。 -
A bt - e. coli shuttle vector pht315 was deleted its replication region of bt, then constructed a novel vector named pht315 - 1 which composed a multiple cloning site, erythromycin and ampicillin - resistance marker and could only replicated in e. coli. used pht315 - 1, a 5273 bps dna fragment carrying a novel bt plasmid replicon was isolated and registered in genbank as ay278324. sequence analysis showed that there were at least three orf ( open reading frame ) in the cloned dna encoding 501, 333, 183aas. orfl had 98 % identities with replicating related protein ori43 of bt strain hd263. the others were no homology to any published bt replicating related protein. after continuous cultured for 70h at 30 c without antibiotic selecting press. the stability of plasmid carrying cloned replicon in bt acrystalliferous mutant strain hd73 cry was more than 98 %. and growth curve also showed that the novel replicon was stable and could replicate normally
進一步序列分析表明該復制區至少有3個較大的orf ，分別編碼501 ， 333 ， 183個氨基酸。其中orf1蛋白序列與hd263復制蛋白ori43的同源性為98 ，而另外兩個orf和genbank己公布的bt復制相關蛋白無同源性。 30連續培養72h ，復制區質粒在bt無晶體突變株hd73cry ~ -中穩定性達98以上， 30h生長曲線也表明該復制區能夠在bt中穩定復制和遺傳，對受體菌株無明顯不良影響。 -
In order to further investigate the role of axudl in human tumor carcinogenesis and the potential association between the axudl gene expression status and the stimulation of transforming growth factor beta in human cancers, the present study was performed in three aspects as follows : ( 1 ) cloning full length enconding region cdna of axudl and construction of eukaryotic vector that expression the fusion protein of axud1 and influenza virus hemagglutin ha epitope tag ; ( 2 ) exploring the time and dose effects of tgf - 1 on the expression - of axudl gene in hepg2 hepatoma cells and spc - a1 lung carcinomas cells, and studying the effects of overexpression of axud1 on the expression of cell cycle and apoptosis related protein in hepg2 hepatoma cells ; ( 3 ) construction and expression of human axudl in e. coli m15. the following main results and conclusions can be obtained from the present study : 1. the full length ecnoding region of human axudl cdna from human peripheral blood lymphocytes was successfully cloned using one step rt - pcr method, and constructed into a eukaryotic expression vector which can be expressed a ha - axud1 fusion protein with axud1 and influenza virus hemagglutin ha epitope tag. the recombinant plasmid was identified by polymerase chain reaction, restriction endonuclease maping and sequencing, this expression vector might be instrumental to further study the function of axud1 protein in tumor cells
為了進一步研究axud1在人類腫瘤發生中的作用及axud1基因的表達狀況與tgf -介導的信號通路的關系，本實驗研究分為三個部分： （ 1 ） axud1基因cdna全長編碼區的克隆和ha表位標記的axud1基因表達載體的構建； （ 2 ）探討肝癌細胞hepg2和肺腺癌spc - a1細胞中tgf - 1誘導的axud1基因表達的時間、劑量效應以及誘導表達的可能機理，並研究axud1的過表達對細胞周期和細胞凋亡相關蛋白表達的影響； （ 3 ） axud1原核表達載體的構建及其在大腸桿菌中的表達。本實驗的主要結果和結論如下： 1利用一步法rt - pcr成功地從人類外周血淋巴細胞中克隆出axud1基因編碼區cdna ，並將其構建入真核表達載體中，編碼的ha - axud1融合蛋白帶有流感病毒凝血素ha的表位標記肽段。 -
The ubi - sl - tocs fragment was taken out, inserted into the multi - cloning site of pcambia1300 vector, transformed into jm109 strain finally, positive colonies were screened on lb plate ( 60 g / ml kan added ). the result of pcr and enzyme digestion of plasmid proved that recombinatin vector was obtained ( named pcusaib4 and pcusaibu )
把擴增產物分別通過clai和bamhi酶切純化，連接到用clai和bamhi切去gfpml基因的中間表達載體pugfpocs中，轉化大腸桿菌jm109 ，在含amp （ 100 g / ml ）的lb抗性平板上篩選到了的陽性菌落。 -
Sequence analysis shows that they share 98. 75 % similarity at the dna, and 98. 67 % at the protein level. ns2 gene was cloned into the multiple cloning sites of prokaryotic expression vector pgex - 6p - l. the recombinant plasmid pgex - 6p - ns2 was constructed and transformed to the competent cell bl21 ( de3 ) plyss, positive bacterium strain was induced by iptg
將ns2基因插入到原核表達性質粒pgex - 6p - 1的ecor 、 bamh多克隆位點之間，將重組原核表達質粒pgex - 6p - ns2轉化到bl21 （ de3 ） plyss感受態細胞中，獲得了表達ns2基因的陽性亞克隆重組子，在含amp的lb液體培養基中培養，經iptg誘導表達，用sds - page分析表達產物。
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