plasmid replicon 中文意思是什麼
-
A bt - e. coli shuttle vector pht315 was deleted its replication region of bt, then constructed a novel vector named pht315 - 1 which composed a multiple cloning site, erythromycin and ampicillin - resistance marker and could only replicated in e. coli. used pht315 - 1, a 5273 bps dna fragment carrying a novel bt plasmid replicon was isolated and registered in genbank as ay278324. sequence analysis showed that there were at least three orf ( open reading frame ) in the cloned dna encoding 501, 333, 183aas. orfl had 98 % identities with replicating related protein ori43 of bt strain hd263. the others were no homology to any published bt replicating related protein. after continuous cultured for 70h at 30 c without antibiotic selecting press. the stability of plasmid carrying cloned replicon in bt acrystalliferous mutant strain hd73 cry was more than 98 %. and growth curve also showed that the novel replicon was stable and could replicate normally
進一步序列分析表明該復制區至少有3個較大的orf ，分別編碼501 ， 333 ， 183個氨基酸。其中orf1蛋白序列與hd263復制蛋白ori43的同源性為98 ，而另外兩個orf和genbank己公布的bt復制相關蛋白無同源性。 30連續培養72h ，復制區質粒在bt無晶體突變株hd73cry ~ -中穩定性達98以上， 30h生長曲線也表明該復制區能夠在bt中穩定復制和遺傳，對受體菌株無明顯不良影響。 -
Construction of two vectors containing different plasmid original replicon this work constructed four resolution shuttle vectors, pbmb1205, pbmb1205r, pbmb1206 and pbmb1206r. there are multiple clone sites between two copies of res sites
解離載體的構建和性能利用來源於不同質粒上的質粒復制起始區ori44和ori1030構建了4個解離載體。 -
For vector pbmb1205 and pbmb1205r, the plasmid original replicon ori44 was come from b. thuringiensis subsp
它們的構建為今後構建高毒力基因工程菌提供了很好的材料。 -
Kurstaki strain hd263. the plasmid original replicon ori1030 of vector pbmb1206 and pbmb1206r was cloned from b. thuringiensis subsp. kurstaki strain hd73
該類載體的構建在國內尚無人報道，國外也沒有成熟的實用性好的解離載體的報道。
分享友人