replicon 中文意思是什麼

replicon 解釋
復制子：一個復制單位，包括真核的復制起點和位於兩側的復制終點之間的區域。

  1. Each segment of dna thus synthesized is called a replicon.

    這樣合成的每一片段DNA稱為復制子。
  2. Considering alcaligenes faecalis pencillin g acylase ( afpga ), which possesses the attractive characteristics for beta - lactam antibiotics conversions, the gene of pga was cloned into two expressing vector pkkfpga and psmlfpga. both the constructed plasmids psmlfpga and pkkfpga contained the pga gene, trc promoter and rrnb transcript terminator, differ in the replicon and antibotic marker, pkkfpga contained multicopy replicon ( cole 1 ) and ampicillin marker. while psmlfpga contained medium - copy replicon ( p15a ) and tetracycline marker

    本文將糞產堿桿菌青霉素g酰化酶（ afpga ）基因構建表達載體pkkfpga和psmlfpga ， pkkfpga和psmlfpga均含trc啟動子、青霉素g酰化酶基因、 rrnb轉錄終止子，其中pkkfpga含有氨卞青霉素抗性基因和cole1高拷貝復制子；而psmlfpga含有四環素抗性基因和p15a中拷貝復制子。
  3. A bt - e. coli shuttle vector pht315 was deleted its replication region of bt, then constructed a novel vector named pht315 - 1 which composed a multiple cloning site, erythromycin and ampicillin - resistance marker and could only replicated in e. coli. used pht315 - 1, a 5273 bps dna fragment carrying a novel bt plasmid replicon was isolated and registered in genbank as ay278324. sequence analysis showed that there were at least three orf ( open reading frame ) in the cloned dna encoding 501, 333, 183aas. orfl had 98 % identities with replicating related protein ori43 of bt strain hd263. the others were no homology to any published bt replicating related protein. after continuous cultured for 70h at 30 c without antibiotic selecting press. the stability of plasmid carrying cloned replicon in bt acrystalliferous mutant strain hd73 cry was more than 98 %. and growth curve also showed that the novel replicon was stable and could replicate normally

    進一步序列分析表明該復制區至少有3個較大的orf ，分別編碼501 ， 333 ， 183個氨基酸。其中orf1蛋白序列與hd263復制蛋白ori43的同源性為98 ，而另外兩個orf和genbank己公布的bt復制相關蛋白無同源性。 30連續培養72h ，復制區質粒在bt無晶體突變株hd73cry ~ -中穩定性達98以上， 30h生長曲線也表明該復制區能夠在bt中穩定復制和遺傳，對受體菌株無明顯不良影響。
  4. Construction of two vectors containing different plasmid original replicon this work constructed four resolution shuttle vectors, pbmb1205, pbmb1205r, pbmb1206 and pbmb1206r. there are multiple clone sites between two copies of res sites

    解離載體的構建和性能利用來源於不同質粒上的質粒復制起始區ori44和ori1030構建了4個解離載體。
  5. Each segment of dna thus synthesized is called a replicon

    這樣合成的每一片段dna稱為復制子。
  6. For vector pbmb1205 and pbmb1205r, the plasmid original replicon ori44 was come from b. thuringiensis subsp

    它們的構建為今後構建高毒力基因工程菌提供了很好的材料。
  7. Kurstaki strain hd263. the plasmid original replicon ori1030 of vector pbmb1206 and pbmb1206r was cloned from b. thuringiensis subsp. kurstaki strain hd73

    該類載體的構建在國內尚無人報道，國外也沒有成熟的實用性好的解離載體的報道。
  8. Therefore, the 4. 4kb fragment from pbl - 2 was ligated to a shuttle vector pht304 with bt replicon and transformed into acrystalliferous mutant 4d10 ( serotype hm, ) and resulted in a cloned strain bl - 3. this strain could express only 130kda protein

    它不僅對蘇雲金芽胞桿菌工程菌的構建具有現實意義，而且對其它基因高表達研究將產生積極影響，具有一定的理論意義和潛在的應用價值
分享友人
分享到 Line

分享到臉書